Isothermal Calorimetric Studies on Human HDL Apolipoproteins
Isothermal calorimetry measures the enthalpy change involved in a chemical reaction. When adapted to the microscale, it quantitates the amount of millijoules associated at a given temperature with the mixing and reassembly of biochemicals, i.e., in our case an apoprotein solution and a phospholipid dispersion. The extent of reassembly and the complex composition can be derived from density gradient ultracentrifugation experiment performed in parallel with the microcalorimetric measurements (Rosseneu et al., 1974).
KeywordsAlbumin Enthalpy Tyrosine Titration Lysine
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