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Einfluß von Albumin und Substratmenge auf den Testosteronstoffwechsel in der isoliert perfundierten Meerschweinchenleber

  • W. Staib
  • K. Demisch
  • P. Birkelbach
  • U. Ammedick
Part of the Oestrogene Hypophysentumoren book series (ENDOKRINOLOGIE, volume 15)

Influence of Substrate Ratio and Albumin on Testosterone Metabolism in Isolated Perfusion of Guinea Pig Liver

Summary

Isolated guinea-pig-livers were perfused in single passage with large doses of 4–14C-Testo-sterone (7,2 mg; spez. act. 1,1 μc/mg) in Krebs-Ringer-Bicarbonate solution. The following metabolites were isolated from the perfusion medium: 6β -Hydroxytestosterone, 6β-Hydroxy-androstenedione, 7α-Hydroxytestosterone, 7α -Hydroxyandrostenedione, Androst-4-ene-3,17-dione, 5β-Androstane-3α,17β -diol and 5β -Androstane-3α -ol-17-one. These compounds have been characterized by their behaviour on a SE-30 and XE-60 column, as free steroids, steroid-acetates and steroid-trimethylsilylethers.

In other experiments the administration of 2,6 g of albumin to 100 ml perfusion medium produces a 20% rise in the amount of free metabolites of testosterone which could be extracted from the perfusing fluid. There is also a significant increase in production of Androstenedione. Other proteins like Haemacel or γ -Globulin don’t cause any significant change in the metabolism of large doses of testosterone. No 17-Hydroxysteroid-dehydrogenase-activity has been observed in the used albumin.

Liver perfusion studies, using small amounts of testosterone (30 μg) have shown a markedly increase of the production of steroidconjugates. In this fraction Hydroxymetabolites of testosterone and 5β -Androstane- α, 17β -diol could be found.

Literatur

  1. 1.
    Brown-Grant, K., E. Forchielli, and R. I. Dorfman: J. biol. Chem. 235, 1317 (1960).PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1969

Authors and Affiliations

  • W. Staib
    • 1
  • K. Demisch
    • 1
  • P. Birkelbach
    • 1
  • U. Ammedick
    • 1
  1. 1.Physikalisch-Chimische InstitutUniversität DüsseldorfDeutschland

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