In vivo Perfusion eines menschlichen Ovars mit 7α-3H-Pregnenolon-sulfat
Perfusion of a Human Ovary by 7α-3H-Pregnenolone-Sulfatein vivo
The substrate was injected into an ovarian artery in vivo. Over 15 min. the ovarian veinons blood was collected and analyzed for free and conjugated steroids. In plasma 1.6%, in ovarian tissue 12% of total radioactivity were found to be free steroids, indicating sulfatase activity. In this fraction only progesterone and androstenedione besides pregnenolone could be isolated. After solvolysis of sulfoconjugates only delta-5-metabolites such as dehydroepi-androsterone and androstenediol were detected. It was concluded that free and sulfoconjugated pregnenolone underwent different metabolism.