In vivo Perfusion eines menschlichen Ovars mit 7α-3H-Pregnenolon-sulfat

  • P. Knapstein
  • L. Beck
  • G. W. Oertel
Part of the Oestrogene Hypophysentumoren book series (ENDOKRINOLOGIE, volume 15)

Perfusion of a Human Ovary by 7α-3H-Pregnenolone-Sulfatein vivo

Summary

The substrate was injected into an ovarian artery in vivo. Over 15 min. the ovarian veinons blood was collected and analyzed for free and conjugated steroids. In plasma 1.6%, in ovarian tissue 12% of total radioactivity were found to be free steroids, indicating sulfatase activity. In this fraction only progesterone and androstenedione besides pregnenolone could be isolated. After solvolysis of sulfoconjugates only delta-5-metabolites such as dehydroepi-androsterone and androstenediol were detected. It was concluded that free and sulfoconjugated pregnenolone underwent different metabolism.

Copyright information

© Springer-Verlag Berlin · Heidelberg 1969

Authors and Affiliations

  • P. Knapstein
    • 1
  • L. Beck
    • 1
  • G. W. Oertel
    • 1
  1. 1.Universitäts-Frauenklinik MainzDeutschland

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