Contribution to the Sedative Action of Substance P

  • P. Stern
Conference paper


We (Stern and Dobrić, 1957b), have put forward the hypothesis that substance P (SP) is a physiological tranquilizer. This was based particularly on the work of Zetler (1956a), and of Euler and Pernow (1956). Moreover, we were able to demonstrate some synergism between SP and mephenesin (Stern et al., 1957), and other central relaxants (Stern and Dobrić, 1957b). Some of our experiments support this hypothesis. Thus, SP tranquilizes Betta splendens fishes (Stern and Huković, 1958) and wild rabbits (Stern and Milin, 1957), ana suppresses the psychomotor anxiety produced by iminodipropionitrile (Stern and Dobric, 1957a). This view is also supported by findings of Krivoy and Kroeger (1963). During this period, Franz et al. (1961) isolated SP of a purity of 30,000 µ/mg. Even purer SP was prepared by Zuber and Jaques (1962) and by Vogler et al. (1962).Purity was assessed by the contraction of the guinea pig ileum in the presence of antagonists of acetylcholine, histamine, and serotonin. Haefely et al. (1962) injected SP of a purity of 24,000 µ/mg into the ventricles of cats, and even purer SP into mice parenterally, without obtaining a sedative effect.


Sedative Effect Sedative Action Wild Rabbit Central Relaxant Betta Splendens 
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  1. Euler, U. S. von, and J. H. Gaddum: An unidentified depressor substance in certain tissue extracts. J. Physiol. (Lond.) 72, 74 (1931).Google Scholar
  2. Euler, U. S. von, and B. Pernow: Neurotropic effects of Substance P. Acta physiol. scand. 36, 265 (1956).CrossRefGoogle Scholar
  3. Feldberg,W., and S. L. Sherwood: Injections of drugs into the lateral ventricle of the cat. J. Physiol. (Lond.) 123, 146 (1954).Google Scholar
  4. Franz, J., R. A. Boissonnas, and E. Stürmer: Isolierung von Substanz P aus Pferdedarm und ihre biologische und chemische Abgrenzung gegenüber Bradykinin. Helv. chim. Acta 44, 881 (1961).Google Scholar
  5. Haefely,.„ and A. Hürlimann: Substance P, highly active naturally occurring polypeptide. Experientia (Basel) 18, 297 (1962).CrossRefGoogle Scholar
  6. Haefely, W., H. Thoenen, and A. Hürlimann: The effect of intraventricular application of Substance P preparations of different purity. Med. exp. (Basel) 7, 245 (1962).CrossRefGoogle Scholar
  7. Horovitz, Z., A. R. Furgiuele, L. J. Brannick, J. C. Burke, and B. N. Craver: A new chemical structure with specific depressant effects on the amygdala and on the hyperirritability of the “septal rat.” Nature (Lond.) 200, 369 (1963).Google Scholar
  8. Hukovic, S., and P. Stern: Antagonisten der Substanz P. Naunyn-Schmiedeberg’s Arch, exp. Path. Pharmak. 245, 281 (1963).Google Scholar
  9. Krivoy, W., and D. Kroeger: The neurogenic activity of high potency Substance P. Experientia (Basel) 119, 366 (1963).CrossRefGoogle Scholar
  10. Laszlo, I.: Interference by adenine nucleotides and potassium in the Substance P estimation in different parts of rat brain. J. Physiol. (Lond.) 172, 30P (1964).Google Scholar
  11. Lembeck, F.: Untersuchung über die Auslösung afferenter Im-pulse. Naunyn-Schmiedeberg’s Arch, exp. Path. Pharmak. 230, 1 (1957).CrossRefGoogle Scholar
  12. Serafimov, N., and P. Stern: Influence des Mediateurs chimiques sur la teneur en “Substances P” du cerveau du rat. Arch, int. Physiol. 66, 653 (1956).Google Scholar
  13. Stern, P., and V. Dobric: Beitrag zur sedativen Wirkung der Substanz P. Naturwissenschaften 44, 517 (1957a).CrossRefGoogle Scholar
  14. Stern, P., and V. Dobric: Über die Wirkung der Substanz P im Zentralnervensystem, in Psychotropic Drugs. Amsterdam: Elsevier (1957b).Google Scholar
  15. Stern, P., V. Dobric, and D. Mitroviö-Kocic: Synergistiche Wirkung von Substanz P und Mephenesin. Arch, int. Pharmacodyn. 112, 102 (1957).Google Scholar
  16. Stern, P., and S. Hukovic: Über die inhibitorische Wirkung der Substanz P an kampflustigen Fischen Betta splendens. Naturwissenschaften 45, 626 (1958).CrossRefGoogle Scholar
  17. Stern, P., and S. Hukovic: Beziehungen zwischen zentraler und peripherer Wirkung der Substanz P. Med, exp. (Basel) 2, 1 (1960).CrossRefGoogle Scholar
  18. Stern, P., and R. Milim: Tranquilizing effect of “Substance P.” Proc. Soc. exp. Biol. (N.Y.) 101, 298 (1959).Google Scholar
  19. Sulser, F., M. H. Bickel, and B. B. Brodie: The action of desmethylimipramine in counteracting sedation and cholinergic effects of reserpine-like drugs. J. Pharmacol, exp. Ther. 144, 321 (1964).Google Scholar
  20. Vogler, K., W. Haefely, A. Hürlimann, R. Studer, W. Lergier, R. Strässle, and K. Berneis: A new purification procedure and biological properties of Substance P. Ann. N.Y. Acad. Sci. 104, 378 (1962).CrossRefGoogle Scholar
  21. Yen, C. Y., L. Stanger, and N. Millman: Ataractic suppression of isolation-induced aggressive behavior. Arch, int. Pharmacodyn. 123, 179 (1959).Google Scholar
  22. Zetler, G.: Substanz P, ein Polypeptid aus Darm und Gehirn mit depressiven, hyperalgetischen und morphinantagonistischen Wirkungen auf das Zentralnervensystem. Naunyn-Schmiedeberg’s Arch, exp. Path. Pharmak. 228, 513 (1956a).Google Scholar
  23. Zetler, G.: Wirkungsunterschied zwischen den Polypeptiden Bradykinin und Substanz P am Zentralnervensystem. Naunyn-Schmiedeberg’s Arch, exp. Path. Pharmak. 229, 148 (1956b).Google Scholar
  24. Zuber, H., and R. Jaques: Isolierung von Substanz P aus Rinderhirn. Angew. Chem. 74, 216 (1962).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1966

Authors and Affiliations

  • P. Stern
    • 1
  1. 1.Department of PharmacologyMedical FacultySarajevoYugoslavia

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