Substance P in Lipid Extracts of Brain Tissue

  • F. Lembeck
  • A. Heizmann
  • G. Seidel
Conference paper


The common method of extraction of substance P from nervous tissue or gut employs a technique which was used by Starling for the extraction of secretin from the dog duodenum. Starling boiled the ground tissue in dilute acid, and followed this by neutralization to precipitate the proteins. Such an extract from brain or gut contains substance P and can be used for the bioassay.


Aluminum Oxide Column Precipitation Precipitation Adenosine Compound Ammonium Sulfate Ammonium Oxytocic Activity 
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Copyright information

© Springer-Verlag New York Inc. 1966

Authors and Affiliations

  • F. Lembeck
    • 1
  • A. Heizmann
    • 1
  • G. Seidel
    • 1
  1. 1.Department of PharmacologyUniversity of TübingenGermany

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