Statistical Analysis of the Coding Capacity of Complementary DNA Strands

  • A. Cascino
  • V. Scarlato
  • N. Barni
  • M. Cipollaro
  • A. Franzè
  • M. F. Macchiato
  • G. Pierno
  • A. Tramontano
Conference paper
Part of the Lecture Notes in Biomathematics book series (LNBM, volume 57)

Abstract

A method that allows a statistical evaluation of the protein coding capacity of any sequenced DNA fragment is presented.

The method, applied to a limited number of protein coding gene sequences, allows the identification of some hypothetical protein genes which are coded by the the DNA strand complementary to the coding DNA strand. The finding that some proteins are indeed encoded on opposite DNA strands and that the two genes have complementary codon sequences, led us to investigate a much larger sample of DNA sequences, included in our DNA data bank.

The results of this exhaustive investigation demonstrate the existence of a new class of protein coding gene sequences that are coded by the complementary DNA strand.

Keywords

Codon Recombination Polypeptide Kelly Gingeras 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cascino, A., Cipollaro, M., Guerrini, A.M., Spena, A. and Scarlato, V. (1981) Nucl. Acids Res. 9 (6), 1499–1518CrossRefGoogle Scholar
  2. 2.
    Scarlato, V., Barni, N., Cipollaro, M., Franzè, A., Macehiato, M.F., Pierno, G., Tramontano, A. and Cascino, A. (1983) Nucl. Acids Res. (submitted)Google Scholar
  3. 3.
    Tramontano, A., Scarlato, V., Barni, N., Cipollaro, M., Franzè, A., Macchiato, M.F., and Cascino, A. (1983) Nucl. Acids Res. (submitted)Google Scholar
  4. 4.
    Rak, B., Lusky, M. and Hable, M. (1982) Nature 297, 124–159CrossRefGoogle Scholar
  5. 5.
    Gingeras, T.R., Sciaky, D., Gelinas, R.E., Bing-Dong, J., Yen, C.E., Kelly, P.A., Bullock, P.A., Parsons, B.L., O’Neill, K.E. and Roberts, R.J. (1982) J. Mol. Biol. 257 (22), 13475–13491.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • A. Cascino
    • 1
  • V. Scarlato
    • 1
  • N. Barni
    • 1
  • M. Cipollaro
    • 3
  • A. Franzè
    • 1
  • M. F. Macchiato
    • 2
  • G. Pierno
    • 1
  • A. Tramontano
    • 1
  1. 1.International Institute of Genetics and Biophysics, C.N.R., 2nd Medical SchoolUniversity of NaplesNaplesItaly
  2. 2.Institute of Physics, 2nd Medical SchoolUniversity of NaplesNaplesItaly
  3. 3.III Servizio Analisi, 2nd Medical SchoolUniversity of NaplesNaplesItaly

Personalised recommendations