Abstract
Several lines of evidence have recently begun to suggest that allelic variants detected by electrophoresis may be heterogeneous, discrete elec-trophoretic variants actually representing a collection of alleles each migrating to the same position on a gel. In this paper I shall describe a series of investigations which I have carried out over the last six years employing gel sieving analysis to detect and characterize electro-phoretically cryptic variation. These investigations have been reported in a series of publications (Johnson 1971, 1972, 1974, 1975a, 1975b, 1976a, 1977a, 1977b, and 1977c), which discuss in detail the theory and experimental methods of this approach.
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References
Chrambach, A., and D. Rodbard. 1971. Polyacrylamide gel electrophoresis. Science 172: 440–451.
Enama, M. 1977. Genetic variation in PEP and RuDP carboxylase in Distchlis spicata. Carnegie Institution Year Book 75, in press.
Fawcett, J., and C. Morris. 1966. Molecular-sieve chromatography of proteins on granulated Polyacrylamide gels. Separation Science 1: 9–26.
Ferguson, K. 1964. Starch gel electrophoresis: Application to the classification of pituitary proteins and polypeptides. Metabolism 13: 985–1002.
Gonnenne, A., and J. Lebowitz. 1975. Estimation of molecular weights of small proteins on Polyacrylamide gel electrophoresis. Anal. Biochem. 64: 414–424.
Johnson, G. 1971. Analysis of enzyme variation in natural populations of the butterfly Colias eurytheme. Proc. Nat. Acad. Sci. USA 68: 997–1001.
Johnson, G. 1972. The selective significance of biochemical polymorphisms in Colias butterflies. Ph.D. thesis, Stanford University, Stanford, California, USA.
Johnson, G. 1974. Enzyme polymorphism and metabolism. Science 184: 28–37.
Johnson, G. 1975a. Use of internal standards in electrophoretic surveys of enzyme polymorphism. Biochemical Genetics 13: 833–847.
Johnson, G. 1975b. On the estimation of effective number of alleles from electrophoretic data. Genetics 78: 771–776.
Johnson, G. 1976a. Hidden alleles at the α-glycerophosphate dehydrogenase locus in Colias butterflies. Genetics 83: 149–167.
Johnson, G. 1976b. Polymorphism and predictability at the α-glycerophosphate dehydrogenase locus in Colias butterflies: Gradients in allele frequency within single populations. Biochemical Genetics 14: 403–426.
Johnson, G. 1977a. Characterization of electrophoretically cryptic variation in the alpine butterfly Colias meadii. Biochemical Genetics 15: 665–693.
Johnson, G. 1977b. Evaluation of the stepwise mutation model of electrophoretic mobility: Comparison of the gel sieving behavior of alleles at the esterase-5 locus of Drosophila melanogaster. Genetics, in press.
Johnson, G. 1977c. Hidden heterogeneity at the esterase C locus of cactus Drosophila. Submitted to Nature.
Johnson, G. 1977d. Isozymes, allozymes, and enzyme polymorphisms: Structural constraints on polymorphic variation. In Isozymes: Current Topics in Biological and Medical Research. (Rattazzi, M., J. Scandalios, and G. Whitt, eds.). Liss Publ., Inc., N.Y., in press.
Nei, M., R. Chakraborty, and P. Fuerst. 1977. The infinite allele model with varying mutation rate. Proc. Nat. Acad. Sci. USA, in press.
Rodbard, D., and A. Chrambach. 1970. Unified theory for gel electrophoresis and gel filtration. Proc. Nat. Acad. Soi. USA 65: 970–977.
Rodbard, D., and A. Chrambach. 1971. Estimation of molecular radius, free mobility, and valence using Polyacrylamide gel electrophoresis. Anal. Biochem. 40: 95–134.
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Johnson, G.B. (1977). Hidden Heterogeneity among Electrophoretic Alleles. In: Christiansen, F.B., Fenchel, T.M. (eds) Measuring Selection in Natural Populations. Lecture Notes in Biomathematics, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-93071-3_13
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DOI: https://doi.org/10.1007/978-3-642-93071-3_13
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