Skip to main content

Hidden Heterogeneity among Electrophoretic Alleles

  • Conference paper
Measuring Selection in Natural Populations

Part of the book series: Lecture Notes in Biomathematics ((LNBM,volume 19))

Abstract

Several lines of evidence have recently begun to suggest that allelic variants detected by electrophoresis may be heterogeneous, discrete elec-trophoretic variants actually representing a collection of alleles each migrating to the same position on a gel. In this paper I shall describe a series of investigations which I have carried out over the last six years employing gel sieving analysis to detect and characterize electro-phoretically cryptic variation. These investigations have been reported in a series of publications (Johnson 1971, 1972, 1974, 1975a, 1975b, 1976a, 1977a, 1977b, and 1977c), which discuss in detail the theory and experimental methods of this approach.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Chrambach, A., and D. Rodbard. 1971. Polyacrylamide gel electrophoresis. Science 172: 440–451.

    Article  PubMed  CAS  Google Scholar 

  • Enama, M. 1977. Genetic variation in PEP and RuDP carboxylase in Distchlis spicata. Carnegie Institution Year Book 75, in press.

    Google Scholar 

  • Fawcett, J., and C. Morris. 1966. Molecular-sieve chromatography of proteins on granulated Polyacrylamide gels. Separation Science 1: 9–26.

    Article  CAS  Google Scholar 

  • Ferguson, K. 1964. Starch gel electrophoresis: Application to the classification of pituitary proteins and polypeptides. Metabolism 13: 985–1002.

    Article  PubMed  CAS  Google Scholar 

  • Gonnenne, A., and J. Lebowitz. 1975. Estimation of molecular weights of small proteins on Polyacrylamide gel electrophoresis. Anal. Biochem. 64: 414–424.

    Article  Google Scholar 

  • Johnson, G. 1971. Analysis of enzyme variation in natural populations of the butterfly Colias eurytheme. Proc. Nat. Acad. Sci. USA 68: 997–1001.

    Article  CAS  Google Scholar 

  • Johnson, G. 1972. The selective significance of biochemical polymorphisms in Colias butterflies. Ph.D. thesis, Stanford University, Stanford, California, USA.

    Google Scholar 

  • Johnson, G. 1974. Enzyme polymorphism and metabolism. Science 184: 28–37.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, G. 1975a. Use of internal standards in electrophoretic surveys of enzyme polymorphism. Biochemical Genetics 13: 833–847.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, G. 1975b. On the estimation of effective number of alleles from electrophoretic data. Genetics 78: 771–776.

    Google Scholar 

  • Johnson, G. 1976a. Hidden alleles at the α-glycerophosphate dehydrogenase locus in Colias butterflies. Genetics 83: 149–167.

    PubMed  CAS  Google Scholar 

  • Johnson, G. 1976b. Polymorphism and predictability at the α-glycerophosphate dehydrogenase locus in Colias butterflies: Gradients in allele frequency within single populations. Biochemical Genetics 14: 403–426.

    Article  CAS  Google Scholar 

  • Johnson, G. 1977a. Characterization of electrophoretically cryptic variation in the alpine butterfly Colias meadii. Biochemical Genetics 15: 665–693.

    Article  CAS  Google Scholar 

  • Johnson, G. 1977b. Evaluation of the stepwise mutation model of electrophoretic mobility: Comparison of the gel sieving behavior of alleles at the esterase-5 locus of Drosophila melanogaster. Genetics, in press.

    Google Scholar 

  • Johnson, G. 1977c. Hidden heterogeneity at the esterase C locus of cactus Drosophila. Submitted to Nature.

    Google Scholar 

  • Johnson, G. 1977d. Isozymes, allozymes, and enzyme polymorphisms: Structural constraints on polymorphic variation. In Isozymes: Current Topics in Biological and Medical Research. (Rattazzi, M., J. Scandalios, and G. Whitt, eds.). Liss Publ., Inc., N.Y., in press.

    Google Scholar 

  • Nei, M., R. Chakraborty, and P. Fuerst. 1977. The infinite allele model with varying mutation rate. Proc. Nat. Acad. Sci. USA, in press.

    Google Scholar 

  • Rodbard, D., and A. Chrambach. 1970. Unified theory for gel electrophoresis and gel filtration. Proc. Nat. Acad. Soi. USA 65: 970–977.

    Article  CAS  Google Scholar 

  • Rodbard, D., and A. Chrambach. 1971. Estimation of molecular radius, free mobility, and valence using Polyacrylamide gel electrophoresis. Anal. Biochem. 40: 95–134.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1977 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Johnson, G.B. (1977). Hidden Heterogeneity among Electrophoretic Alleles. In: Christiansen, F.B., Fenchel, T.M. (eds) Measuring Selection in Natural Populations. Lecture Notes in Biomathematics, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-93071-3_13

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-93071-3_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-08435-8

  • Online ISBN: 978-3-642-93071-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics