Zusammenfassung
Bei der autoradiographischen Lokalisation von Radioelementen erfolgt deren Nachweis mit Hilfe einer Photoemulsion, die in möglichst engem Kontakt mit dem radioaktiven biologischen Objekt, z. B. einem Gewebsschnitt, exponiert wird. Nach beendeter Exposition und anschließendem Entwickeln gibt die Schwärzung in der Photoemulsion Menge und Verteilung des radioaktiven Materials im Gewebe wieder.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literatur
Arnold, J. S.: A method for embedding undecalcified bone for histologic sectioning, and its application to radioautography. Science 114, 178–180 (1951).
Ascenci, A., G. Boato and F. Passalacqua: Presence of organic iodine in the pituitary. Nature (Lond.) 169, 886 (1952).
Bachmann, R., E. Harbers u. K. Neumann: Autoradiographische Untersuchung von Thoriumpräparaten (Peteosthor), Verh. anat. Ges. 48. Versammig. Kiel. Sonderbd. Anat. Anz. 154–163 (1950).
Bélanger, L. F.: A method for routine detection of radiophosphates and other radioactive compounds in tissues. The inverted autograph. Anat. Rec. 107, 149–159 (1950).
— Autoradiographic visualization of the entry and transit of S35 in cartilage, bone, and dentine of young rats and the effect of hyaluronidase in vitro. Canad. J. Biochem. 32, 161–169 (1954).
— and C. P. Leblond: A method for locating radioactive elements in tissues by covering histological sections with a photographic emulsion. Endocrin. 39, 8–13 (1946).
Board, F. A.: Sulfhydryl detection by histochemography. J. cell. comp. Physiol. 38, 377–387 (1951).
Bogoroch, R., M. Israelovitch and C. P. Leblond: The chemical nature of the materials retained in radioautographic preparations of thyroid glands of rats after radioiodine administration. J. Nat. Cancer Inst. 12, 255 (1951).
Boström, H.: Chemical and autoradiographic studies on the sulphate exchange in sulphomucopoly-saccharides. Ark. Kemi 6, 43–57 (1953).
Bourne, G. H.: Autoradiography. Biol. Rev. Cambridge Philos. Soc. 27, 108–131 (1952)
Boyd, G. A.: Autoradiography of C14 incorporated in individual blood cells. Science 108, 529–531 (1948).
— Autoradiography in biology and medicine. New York: Academic Press 1955.
— and F. A. Board: A preliminary report on histochemography. Science 110, 586–588 (1949).
— G. W. Casarett and A. I. Williams: Making autoradiographs of individual blood cells. Stain Technol. 25, 13–16 (1950).
— and H. Levi: C14-β-track autoradiography. Science 111, 58–59 (1950).
Branson, H., and L. A. Hansborough: The quantitative theory of autoradiography illustrated through experiments with P32 in the chick embryo. Science 108, 327–328 (1948).
Buckaloo, G. W., and D. V. Cohn: Color autoradiography. Science 123, 333 (1956).
Campbell, D.: Track autoradiographs with iron59 and sulfur35 with quantitative evaluation. Nature (Lond.) 167, 274–275 (1951).
— and B. H. Persson: Use of track autoradiography in studies on the sulfur metabolism of connective tissues. Experientia (Basel) 7, 304–306 (1951).
Canny, M. J.: High resolution autoradiography of water-soluble substances. Nature (Lond.) 175, 857–858 (1955).
Diserens, H. W., and O. Hall: A method for radioautographic localization of isotopes in tissues. Texas Rep. Biol. a. Med. 10, 286–294 (1952).
Doniach, I., A. Howard and S. R. Pelc: Autoradiography. Progr. Biophys. 3, 1–26 (1953).
— and S. R. Pelc: Autoradiographic technique. Brit. J. Radiol. 23, 184–192 (1950).
Dudley, R. A., and S. R. Pelc: Automatic grain counter for assessing quantitatively high-resulotion autoradiographs. Nature (Lond.) 172, 992–993 (1953).
Dziewiatkowski, D. D.: Radioautographic studies on S35-labeled sulfate-sulfur metabolism in the articular cartilage and bone of suckling rats. Transactions 4th Conference on Metabolic Interrelations, p. 74–99 (1952).
Edwards, L. C.: An adhesive transfer method for cutting and mounting thin sections for autoradiography. Stain Technol. 30, 163–168 (1955).
Engfeldt, B., A. Engström and R. Zetterström: Renewal of phosphate in bone materials. II. Radioautographic studies of the renewal of phosphate in different structures of bone. Biochim. biophys. Acta 8, 375–380 (1952).
Everett, N. B., and B. S. Simmons: Observations on the histochemical reduction of photographic emulsion in radioautography. Anat. Rec. 117, 25–35 (1953).
Feldman, W., and C. H. Waddington: The uptake of methionine-S35 by the chick embryo and its inhibition by ethionine. J. Embryol. exp. Morphol. 3, 44–58 (1955).
Ficq, A.: Êtude autoradiographique du métabolisme des protéines et des acides nucléique au cours de l’oogénèse chez les bactraciens. Exp. Cell Res. 9, 286–293 (1955).
— et J. Brachet: Distribution de l’acide ribonucléique et incorporation de phénylalanine-2-C14 dans les protéines. Exp. Cell Res. 11, 135–145 (1956).
— F. Gavosto et M. Errera: Incorporation in vitro de glycine-1-C14 cellules individuelles de la moelle osseuse. Exp. Cell Res. 6, 69–75 (1954).
Fitzgerald, P. J.: Radioautography, its use in cytology. In “Analytical Cytology” (R. C. Mellors, Edit.). New York: McGraw Hill Book Comp. 1955.
— E. Simmel, J. Weinstein and C. Martin: Radioautography: Theory, technic, and applications. Lab. Invest. 2, 181–222 (1953).
Gallimore, E. R., E. C. Bauer, and G. A. Boyd: A non-leaching technic for autoradiography. Stain Technol. 29, 95–98 (1954).
Goldstein, L., and W. Plaut: Direct evidence for nuclear synthesis of cytoplasmic ribose nucleic acid. Proc. nat. Acad. Sci. (Wash.) 41, 874–880 (1955).
Gomberg, H. J.: A new high-resolution system of autoradiography. Nucleonics 9, No. 4, 28–43 (1951).
Gross, J., R. Bogoroch, N. J. Nadler and C. P. Leblond: The theory and methods of the radioautographic localization of radioéléments in tissues. Amer. J. Roentgenol. 65, 420 bis 458 (1951).
Guidotti, G., and F. Passalacqua: On the preparation of specimens for autoradiography. Experientia (Basel) 12, 117–118 (1956).
Harbers, E.: Autoradiographie als histochemisches Untersuchungsverfahren. Handbuch der Histochemie, Band I, 1, S. 400–598. Stuttgart: Gustav Fischer 1958.
— u. R. Backmann: Autoradiographische Darstellung von Phospho-Proteinen in Gewebs-schnitten mit Hilfe von Radiophosphor. Z. Naturforsch. 10b, 385–388 (1955).
— N. K. Chaudhuri and C. Heidelberger: Studies on fluorinated pyrimidines. VIII. Further biochemical and metabolic investigations. J. biol. Chem. 234, 1255–1262 (1959).
— u. P. Doering: Dosisfragen und Strahlenschädigungen bei Anwendung von Radioisotopen. Klin. Wschr. 1955, 777–784.
— and C. Heidelberger: Studies on nucleic acid biosynthesis in Ehrlich ascites cells suspended in a medium permitting growth. J. biol. Chem. 234, 1249–1254 (1959a).
— Incorporation of labeled ribonucleoside-5′-monophosphates into ribonucleic acid in a cytoplasmic fraktion of rat liverhomogenates. Biochim. biophys. Acta 35, 381–388 (1959b).
— u. K. Neumann: Autoradiographie als histochemische Methodik. Klin. Wschr. 1954, 337–342 (1954 a).
— Grundlagen der autoradiographischen Darstellung der Nucleinsäuren in Gewebs-schnitten mit Hilfe von Radio-Phosphor. Z. Naturforsch. 9b, 175–180 (1954b).
— Quantitativ-chemische Untersuchungen zur färberischen Darstellung der Pentosenucleinsäure in Gewebsschnitten. Z. Naturforsch. 10b, 357–359 (1955).
Herz, R. H.: Photographic fundamentals of autoradiography. Nucleonics 9, No. 3, 24–39 (1951)
Hoecker, F. E., and P. G. Roofe: Structural differences in bone matrix associated with metabolized radium. Radiol. 52, 856–865 (1949).
Holt, M. W., and S. Warren: A radioautographic method for detailed localization of radioactive isotopes in tissues without isotope loss. Proc. Soc. exp. Biol. (N. Y.) 73, 545–549 (1950).
Hornsey, S., and A. Howard: Autoradiographic studies with mouse Ehrlich ascites tumor. Ann. N. Y. Acad. Sci. 63, 915–928 (1956).
Howard, A., and S. R. Pelc: Synthesis of deoxyribose nucleic acid and nuclear incorporation of S35 as shown by autoradiographs, CIBA Foundation Conference on Isotopes in Biochemistry, 138–151. Philadelphia: The Blakistan Co. 1951a.
— Nuclear incorporation of P32 as demonstrated by autoradiographs. Exp. Cell Res. 2, 178–187 (1951b).
Hughes, W. L., V. P. Bond, G. Brecher, E. P. Cronkite, R. B. Painter, H. Quastler and F. G. Sherman: Cellular proliferation in the mouse as revealed by autoradiography with triatiated thymidine. Proc. nat. Acad. Sci. (Wash.) 44, 476–481 (1958).
Johansson, S. A. E., and B. Skanse: A photographic method of determining the distribution of radioactive material in vivo. Acta radiol. (Stockh.) 39, 317–322 (1953).
Kaylor, C. T., and D. C. Van Cleave: Radiographic visualization of the deposition of radio-beryllium in the rat. Anat. Rec. 117, 467–481 (1953).
Kellershon, C., et P. Pellerin: Sur la possibilité d’obtenir Tautoradiographie d’un organe in vivo. C. R. Acad. Sci. (Paris) 239, 111–113 (1954).
Lacroix, P.: Autoradiographies du tissu osseux spongieux. Experientia (Basel) 8, 426 (1952).
Lajtha, L. G.: Detection of adenine carbon-14 in deoxyribonucleic acid by autoradiography. Nature (Lond.) 173, 587 (1954).
Leblond, C. P., and J. Gross: Thyreoglobulin formation in the thyroid follicle visualized by the “coated autograph” technique. Endocrin. 43, 306–324 (1948).
— G. W. Wilkinson, L. F. Bélanger and J. Robichon: Visualization of bone formation in the rat with the help of P32 radioautographs. Amer. J. Anat. 86, 289–341 (1950).
Levi, H.: Quantitative β-track autoradiography of single cells. Exp. Cell Res. 7, 44–51 (1954).
Loehr, W. M.: Chest radioautography. Amer. J. Roentgenol. 68, 355–359 (1952).
London, E. S.: Études sur la valeur physiologique et pathologique de l’émanation du radium. Arch. Elect, méd. 12, 363–372 (1904).
Maloney, M. A., and H. M. Patt: Granulocyte life cycle. Fed. Proc. 17, 406 (1958).
Marinelli, L. D., and R. F. Hill: Radioautography. Some physical and radiobiological aspects of the technique as applied to thin specimens. Amer. J. Roentgenol. 59, 396–403 (1948).
Mayr, J., H. D. Bruner and M. Brucer: Boron detection in tissues using the (n,α) reaction. Nucleonics 11, No. 10, 21–25 (1953).
Mazia, O., W. S. Plaut and G. W. Ellis: A method for the quantitative assessment of auto-radiographs. Exp. Cell Res. 9, 305–312 (1955).
Messier, B., and C. P. Leblond: Preparation of coated radioautographs by dipping sections in fluid emulsion. Proc. Soc. exp. Biol. (N. Y.) 96, 7–10 (1957).
Meyer, K., and M. M. Rapport: The mucopolysaccharides of the ground substance of connective tissue. Science 113, 596–599 (1951).
Miller, B. L., and F. Hoecker: Quantitation of alpha-emitters in bone. Nucleonics 8, No. 5, 44–52 (1951).
Müller, J. H.: Zur klinischen Bedeutung des autoradiographischen Verfahrens bei der Verwendung von kurzlebenden künstlichen radioaktiven Isotopen (Autoorganographie, Auto-angiographie). Experientia (Basel) 4, 406–407 (1948).
— Autoradiographies humaines (Automammagraphies, Autohépatographies, Autoangiographies, etc.) obtenues à l’aide d’un isotope radioactif artificiel (Z63). J. Radiol. Électrol. 30, 290–293 (1949).
Nadler, N. J.: Some theoretical aspects of radioautography. Canad. J. med. Sci. 29, 182–194 (1951).
— The quantitative estimation of radioactive isotopes by radioautography. Amer. J. Roentgenol. 70, 814–823 (1953).
Niklas, A., u. W. Maurer: Autoradiographie, in HOPPE-SEYLER-THIERFELDER, „Handbuch der physiologisch-chemischen Analyse“, Band II, 10. Aufl. Berlin-Göttingen-Heidelberg: Springer 1955.
— W. Oehlert u. Roesch: Autoradiographische Untersuchung der Größe des Eiweißstoff-wechsels verschiedener Organe, Gewebe und Zellarten. Beitr. path. Anat. 116, 92–123 (1956).
Odeblad, E.: Contributions to the theory and technique of quantitative autoradiography with P32 with special reference to the granulosa tissue of the Graafian follicles in the rabbit. Acta radiol. (Stockh.) Suppl. 93 (1951).
— Artefacts in autoradiography. Acta radiol. (Stockh.) 39, 192–204 (1953).
— and C. A. Tobias: Autoradiography after activation of histological sections with deuterons. Arch. Biochem. Biophys. 49, 452 (1954).
Oehlert, W., B. Schultze U. W. Maurer: Autoradiographische Untersuchung der Größe des Eiweißstoffwechsels der verschiedenen Zellen des Zentralnervensystems. Beitr. path. Anat. 119, 343–376 (1958).
Pelc, S. R.: Radiation dose in tracer experiments involving autoradiography, CIBA Foundation Conference on Isotopes in Biochemistry, 138–151. Philadelphia: The Blakiston Co. 1951.
— and A. Howard: Chromosome metabolism as shown by autoradiographs. Exp. Cell Res. Suppl. 2, 269–278 (1952).
— A difference between spermatogonia and somatic tissues of mice in the incorporation of (8-14C)-adenine into deoxyribonucleic acid. Exp. Cell Res. 11, 128–134 (1956).
Plaut, W., and D. Mazia: The distribution of newly synthesized DNA in mitotic division. J. Biophys. biochem. Cytol. 2, 573–587 (1956).
Roofe, P. G., F. E. Hoecker and C. D. Voorhees: A rapid bone sectioning technic. Proc. Soc. exp. Biol. (N. Y.) 72, 619–621 (1949).
Rotblad, J., and G. Ward: Analysis of the radioactive content of tissues by α-track autoradiography. Physics in Med. a. Biol. 1, 57–70 (1956).
Schaefer, H.: Nachweis und Messung kleinster α-Aktivitäten in biologischen Substanzen durch Bahnspurauszählung in der Photoemulsion. Strahlenther. 77, 613–628 (1948).
Schmeiser, K.: Autoradiographie, in „Künstliche radioaktive Isotope in Physiologie, Diagnostik und Therapie“. 1. Aufl., S. 76–92. Berlin-Göttingen-Heidelberg: Springer 1953.
Schneider, W. C.: Phosphorus compounds in animal tissues. I. Extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. J. biol. Chem. 161, 293–303 (1945).
Schultze, B., W. Oehlert u. W. Maurer: Autoradiographische Untersuchung zum Mechanismus der Eiweißneubildung in Ganglienzellen. Beitr. path. Anat. 120, 58–84 (1958).
Siess, M., u. G. Seybold: Das Imprägnierungsverfahren der Autoradiographie von Gewebs-schnitten. Z. wiss. Mikrosk. 63, 156–170 (1957).
Simmel, E. B., P. J. Fitzgerald and J. T. Godwin: Staining of radioautographs with metanil yellow and iron hematoxylin. Stain Technol. 26, 25–28 (1951).
Sognnaes, R. F., J. H. Shaw, A. K. Solomon and E. Harvold: A method for radioautography of specimens composed of both hard and soft structures. Anat. Rec. 104, 319–329 (1949).
Stevens, G. W. W.: Resolution testing in autoradiography. Nature (Lond.) 161, 432–433 (1948).
— Radioactive microphotographs for resolution testing in autoradiography. Brit. J. Radiol. 23, 723–730 (1950).
Taugner, R., U. Wagenmann, H. Hole, G. Grigoleit, R. Droh u. J. Gillissen: Serienmäßige Herstellung von Gefrierschnitt-Autoradiogrammen mit optimalem Kontakt. Naunyn-Schmiedebergs. Arch. exp. Path. Pharmak. 234, 336–342 (1958).
Tauxe, W. N., A. H. Moser and G. A. Boyd: Etymology of autoradiography. Science 120, 149–150 (1954).
Taylor, J. H.: Autoradiographic detection of incorporation of P32 into chromosomes during meiosis and mitosis. Exp. Cell Res. 4, 164–173 (1953).
— Autoradiography at the cellular level. Physical Techniques in Biological Research, Vol. 3, Cells and Tissues. New York: Acad. Press 1956.
— Sister chromatid exchanges in tritium-labeled chromosomes. Genetics 43, 515–529 (1958).
— and R. Mcmaster: Autoradiographic and microphotometric studies of deoxyribose nucleic acid during microgametogenesis in Lilium longiflorum. Chromosoma 6, 489–521 (1954).
— P. S. Woods and W. L. Hughes: The organization and duplication of chromosomes as revealed by autoradiographic studies using Tritium-labeled thymidine. Proc. nat. Acad. Sci. (Wash.) 43, 122–128) (1957).
Webb, J. M.: The reaction of ionizing particles with the photographic emulsion to produce the latent image, Manual for autoradiographic course, Oak Ridge Institute of Nuclear Studies. Oak Ridge, Tenn. 1951.
Williams, A. I.: A method for prevention of leaching and fogging in autoradiographs. Nucleonics 8, No. 6, 10–14 (1951).
Woodruff, L. A., and W. P. Norris: Sectioning of undecalcified bone. With special reference to radioautographic application. Stain Technol. 30, 179–188 (1955).
Editor information
Rights and permissions
Copyright information
© 1961 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Harbers, E. (1961). Autoradiographie. In: Schwiegk, H., Turba, F. (eds) Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie/Radioactive Isotopes in Physiology Diagnostics and Therapy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-92819-2_5
Download citation
DOI: https://doi.org/10.1007/978-3-642-92819-2_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-49048-4
Online ISBN: 978-3-642-92819-2
eBook Packages: Springer Book Archive