Skip to main content

Zusammenfassung

Bei der autoradiographischen Lokalisation von Radioelementen erfolgt deren Nachweis mit Hilfe einer Photoemulsion, die in möglichst engem Kontakt mit dem radioaktiven biologischen Objekt, z. B. einem Gewebsschnitt, exponiert wird. Nach beendeter Exposition und anschließendem Entwickeln gibt die Schwärzung in der Photoemulsion Menge und Verteilung des radioaktiven Materials im Gewebe wieder.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 44.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 59.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  • Arnold, J. S.: A method for embedding undecalcified bone for histologic sectioning, and its application to radioautography. Science 114, 178–180 (1951).

    Article  PubMed  CAS  Google Scholar 

  • Ascenci, A., G. Boato and F. Passalacqua: Presence of organic iodine in the pituitary. Nature (Lond.) 169, 886 (1952).

    Article  Google Scholar 

  • Bachmann, R., E. Harbers u. K. Neumann: Autoradiographische Untersuchung von Thoriumpräparaten (Peteosthor), Verh. anat. Ges. 48. Versammig. Kiel. Sonderbd. Anat. Anz. 154–163 (1950).

    Google Scholar 

  • Bélanger, L. F.: A method for routine detection of radiophosphates and other radioactive compounds in tissues. The inverted autograph. Anat. Rec. 107, 149–159 (1950).

    Article  PubMed  Google Scholar 

  • — Autoradiographic visualization of the entry and transit of S35 in cartilage, bone, and dentine of young rats and the effect of hyaluronidase in vitro. Canad. J. Biochem. 32, 161–169 (1954).

    Article  PubMed  Google Scholar 

  • — and C. P. Leblond: A method for locating radioactive elements in tissues by covering histological sections with a photographic emulsion. Endocrin. 39, 8–13 (1946).

    Article  Google Scholar 

  • Board, F. A.: Sulfhydryl detection by histochemography. J. cell. comp. Physiol. 38, 377–387 (1951).

    Article  CAS  Google Scholar 

  • Bogoroch, R., M. Israelovitch and C. P. Leblond: The chemical nature of the materials retained in radioautographic preparations of thyroid glands of rats after radioiodine administration. J. Nat. Cancer Inst. 12, 255 (1951).

    Google Scholar 

  • Boström, H.: Chemical and autoradiographic studies on the sulphate exchange in sulphomucopoly-saccharides. Ark. Kemi 6, 43–57 (1953).

    Google Scholar 

  • Bourne, G. H.: Autoradiography. Biol. Rev. Cambridge Philos. Soc. 27, 108–131 (1952)

    Article  Google Scholar 

  • Boyd, G. A.: Autoradiography of C14 incorporated in individual blood cells. Science 108, 529–531 (1948).

    Article  PubMed  CAS  Google Scholar 

  • — Autoradiography in biology and medicine. New York: Academic Press 1955.

    Google Scholar 

  • — and F. A. Board: A preliminary report on histochemography. Science 110, 586–588 (1949).

    Article  PubMed  Google Scholar 

  • G. W. Casarett and A. I. Williams: Making autoradiographs of individual blood cells. Stain Technol. 25, 13–16 (1950).

    PubMed  Google Scholar 

  • — and H. Levi: C14-β-track autoradiography. Science 111, 58–59 (1950).

    Article  PubMed  Google Scholar 

  • Branson, H., and L. A. Hansborough: The quantitative theory of autoradiography illustrated through experiments with P32 in the chick embryo. Science 108, 327–328 (1948).

    Article  PubMed  CAS  Google Scholar 

  • Buckaloo, G. W., and D. V. Cohn: Color autoradiography. Science 123, 333 (1956).

    Article  PubMed  CAS  Google Scholar 

  • Campbell, D.: Track autoradiographs with iron59 and sulfur35 with quantitative evaluation. Nature (Lond.) 167, 274–275 (1951).

    Article  CAS  Google Scholar 

  • — and B. H. Persson: Use of track autoradiography in studies on the sulfur metabolism of connective tissues. Experientia (Basel) 7, 304–306 (1951).

    Google Scholar 

  • Canny, M. J.: High resolution autoradiography of water-soluble substances. Nature (Lond.) 175, 857–858 (1955).

    Article  Google Scholar 

  • Diserens, H. W., and O. Hall: A method for radioautographic localization of isotopes in tissues. Texas Rep. Biol. a. Med. 10, 286–294 (1952).

    CAS  Google Scholar 

  • Doniach, I., A. Howard and S. R. Pelc: Autoradiography. Progr. Biophys. 3, 1–26 (1953).

    CAS  Google Scholar 

  • — and S. R. Pelc: Autoradiographic technique. Brit. J. Radiol. 23, 184–192 (1950).

    Article  Google Scholar 

  • Dudley, R. A., and S. R. Pelc: Automatic grain counter for assessing quantitatively high-resulotion autoradiographs. Nature (Lond.) 172, 992–993 (1953).

    Article  CAS  Google Scholar 

  • Dziewiatkowski, D. D.: Radioautographic studies on S35-labeled sulfate-sulfur metabolism in the articular cartilage and bone of suckling rats. Transactions 4th Conference on Metabolic Interrelations, p. 74–99 (1952).

    Google Scholar 

  • Edwards, L. C.: An adhesive transfer method for cutting and mounting thin sections for autoradiography. Stain Technol. 30, 163–168 (1955).

    PubMed  CAS  Google Scholar 

  • Engfeldt, B., A. Engström and R. Zetterström: Renewal of phosphate in bone materials. II. Radioautographic studies of the renewal of phosphate in different structures of bone. Biochim. biophys. Acta 8, 375–380 (1952).

    Article  PubMed  CAS  Google Scholar 

  • Everett, N. B., and B. S. Simmons: Observations on the histochemical reduction of photographic emulsion in radioautography. Anat. Rec. 117, 25–35 (1953).

    Article  PubMed  CAS  Google Scholar 

  • Feldman, W., and C. H. Waddington: The uptake of methionine-S35 by the chick embryo and its inhibition by ethionine. J. Embryol. exp. Morphol. 3, 44–58 (1955).

    Google Scholar 

  • Ficq, A.: Êtude autoradiographique du métabolisme des protéines et des acides nucléique au cours de l’oogénèse chez les bactraciens. Exp. Cell Res. 9, 286–293 (1955).

    Article  PubMed  CAS  Google Scholar 

  • — et J. Brachet: Distribution de l’acide ribonucléique et incorporation de phénylalanine-2-C14 dans les protéines. Exp. Cell Res. 11, 135–145 (1956).

    Article  PubMed  CAS  Google Scholar 

  • F. Gavosto et M. Errera: Incorporation in vitro de glycine-1-C14 cellules individuelles de la moelle osseuse. Exp. Cell Res. 6, 69–75 (1954).

    Article  Google Scholar 

  • Fitzgerald, P. J.: Radioautography, its use in cytology. In “Analytical Cytology” (R. C. Mellors, Edit.). New York: McGraw Hill Book Comp. 1955.

    Google Scholar 

  • E. Simmel, J. Weinstein and C. Martin: Radioautography: Theory, technic, and applications. Lab. Invest. 2, 181–222 (1953).

    PubMed  Google Scholar 

  • Gallimore, E. R., E. C. Bauer, and G. A. Boyd: A non-leaching technic for autoradiography. Stain Technol. 29, 95–98 (1954).

    PubMed  CAS  Google Scholar 

  • Goldstein, L., and W. Plaut: Direct evidence for nuclear synthesis of cytoplasmic ribose nucleic acid. Proc. nat. Acad. Sci. (Wash.) 41, 874–880 (1955).

    Article  CAS  Google Scholar 

  • Gomberg, H. J.: A new high-resolution system of autoradiography. Nucleonics 9, No. 4, 28–43 (1951).

    CAS  Google Scholar 

  • Gross, J., R. Bogoroch, N. J. Nadler and C. P. Leblond: The theory and methods of the radioautographic localization of radioéléments in tissues. Amer. J. Roentgenol. 65, 420 bis 458 (1951).

    CAS  Google Scholar 

  • Guidotti, G., and F. Passalacqua: On the preparation of specimens for autoradiography. Experientia (Basel) 12, 117–118 (1956).

    Article  CAS  Google Scholar 

  • Harbers, E.: Autoradiographie als histochemisches Untersuchungsverfahren. Handbuch der Histochemie, Band I, 1, S. 400–598. Stuttgart: Gustav Fischer 1958.

    Google Scholar 

  • — u. R. Backmann: Autoradiographische Darstellung von Phospho-Proteinen in Gewebs-schnitten mit Hilfe von Radiophosphor. Z. Naturforsch. 10b, 385–388 (1955).

    Google Scholar 

  • N. K. Chaudhuri and C. Heidelberger: Studies on fluorinated pyrimidines. VIII. Further biochemical and metabolic investigations. J. biol. Chem. 234, 1255–1262 (1959).

    PubMed  Google Scholar 

  • — u. P. Doering: Dosisfragen und Strahlenschädigungen bei Anwendung von Radioisotopen. Klin. Wschr. 1955, 777–784.

    Google Scholar 

  • — and C. Heidelberger: Studies on nucleic acid biosynthesis in Ehrlich ascites cells suspended in a medium permitting growth. J. biol. Chem. 234, 1249–1254 (1959a).

    PubMed  Google Scholar 

  • — Incorporation of labeled ribonucleoside-5′-monophosphates into ribonucleic acid in a cytoplasmic fraktion of rat liverhomogenates. Biochim. biophys. Acta 35, 381–388 (1959b).

    Article  PubMed  CAS  Google Scholar 

  • — u. K. Neumann: Autoradiographie als histochemische Methodik. Klin. Wschr. 1954, 337–342 (1954 a).

    Google Scholar 

  • — Grundlagen der autoradiographischen Darstellung der Nucleinsäuren in Gewebs-schnitten mit Hilfe von Radio-Phosphor. Z. Naturforsch. 9b, 175–180 (1954b).

    CAS  Google Scholar 

  • — Quantitativ-chemische Untersuchungen zur färberischen Darstellung der Pentosenucleinsäure in Gewebsschnitten. Z. Naturforsch. 10b, 357–359 (1955).

    CAS  Google Scholar 

  • Herz, R. H.: Photographic fundamentals of autoradiography. Nucleonics 9, No. 3, 24–39 (1951)

    CAS  Google Scholar 

  • Hoecker, F. E., and P. G. Roofe: Structural differences in bone matrix associated with metabolized radium. Radiol. 52, 856–865 (1949).

    CAS  Google Scholar 

  • Holt, M. W., and S. Warren: A radioautographic method for detailed localization of radioactive isotopes in tissues without isotope loss. Proc. Soc. exp. Biol. (N. Y.) 73, 545–549 (1950).

    Article  CAS  Google Scholar 

  • Hornsey, S., and A. Howard: Autoradiographic studies with mouse Ehrlich ascites tumor. Ann. N. Y. Acad. Sci. 63, 915–928 (1956).

    Article  PubMed  CAS  Google Scholar 

  • Howard, A., and S. R. Pelc: Synthesis of deoxyribose nucleic acid and nuclear incorporation of S35 as shown by autoradiographs, CIBA Foundation Conference on Isotopes in Biochemistry, 138–151. Philadelphia: The Blakistan Co. 1951a.

    Google Scholar 

  • — Nuclear incorporation of P32 as demonstrated by autoradiographs. Exp. Cell Res. 2, 178–187 (1951b).

    Google Scholar 

  • Hughes, W. L., V. P. Bond, G. Brecher, E. P. Cronkite, R. B. Painter, H. Quastler and F. G. Sherman: Cellular proliferation in the mouse as revealed by autoradiography with triatiated thymidine. Proc. nat. Acad. Sci. (Wash.) 44, 476–481 (1958).

    Article  CAS  Google Scholar 

  • Johansson, S. A. E., and B. Skanse: A photographic method of determining the distribution of radioactive material in vivo. Acta radiol. (Stockh.) 39, 317–322 (1953).

    Article  CAS  Google Scholar 

  • Kaylor, C. T., and D. C. Van Cleave: Radiographic visualization of the deposition of radio-beryllium in the rat. Anat. Rec. 117, 467–481 (1953).

    Article  PubMed  CAS  Google Scholar 

  • Kellershon, C., et P. Pellerin: Sur la possibilité d’obtenir Tautoradiographie d’un organe in vivo. C. R. Acad. Sci. (Paris) 239, 111–113 (1954).

    Google Scholar 

  • Lacroix, P.: Autoradiographies du tissu osseux spongieux. Experientia (Basel) 8, 426 (1952).

    Article  CAS  Google Scholar 

  • Lajtha, L. G.: Detection of adenine carbon-14 in deoxyribonucleic acid by autoradiography. Nature (Lond.) 173, 587 (1954).

    Article  CAS  Google Scholar 

  • Leblond, C. P., and J. Gross: Thyreoglobulin formation in the thyroid follicle visualized by the “coated autograph” technique. Endocrin. 43, 306–324 (1948).

    Article  CAS  Google Scholar 

  • G. W. Wilkinson, L. F. Bélanger and J. Robichon: Visualization of bone formation in the rat with the help of P32 radioautographs. Amer. J. Anat. 86, 289–341 (1950).

    Article  PubMed  CAS  Google Scholar 

  • Levi, H.: Quantitative β-track autoradiography of single cells. Exp. Cell Res. 7, 44–51 (1954).

    Article  PubMed  CAS  Google Scholar 

  • Loehr, W. M.: Chest radioautography. Amer. J. Roentgenol. 68, 355–359 (1952).

    PubMed  CAS  Google Scholar 

  • London, E. S.: Études sur la valeur physiologique et pathologique de l’émanation du radium. Arch. Elect, méd. 12, 363–372 (1904).

    Google Scholar 

  • Maloney, M. A., and H. M. Patt: Granulocyte life cycle. Fed. Proc. 17, 406 (1958).

    Google Scholar 

  • Marinelli, L. D., and R. F. Hill: Radioautography. Some physical and radiobiological aspects of the technique as applied to thin specimens. Amer. J. Roentgenol. 59, 396–403 (1948).

    CAS  Google Scholar 

  • Mayr, J., H. D. Bruner and M. Brucer: Boron detection in tissues using the (n,α) reaction. Nucleonics 11, No. 10, 21–25 (1953).

    CAS  Google Scholar 

  • Mazia, O., W. S. Plaut and G. W. Ellis: A method for the quantitative assessment of auto-radiographs. Exp. Cell Res. 9, 305–312 (1955).

    Article  PubMed  CAS  Google Scholar 

  • Messier, B., and C. P. Leblond: Preparation of coated radioautographs by dipping sections in fluid emulsion. Proc. Soc. exp. Biol. (N. Y.) 96, 7–10 (1957).

    Article  CAS  Google Scholar 

  • Meyer, K., and M. M. Rapport: The mucopolysaccharides of the ground substance of connective tissue. Science 113, 596–599 (1951).

    Article  PubMed  CAS  Google Scholar 

  • Miller, B. L., and F. Hoecker: Quantitation of alpha-emitters in bone. Nucleonics 8, No. 5, 44–52 (1951).

    PubMed  CAS  Google Scholar 

  • Müller, J. H.: Zur klinischen Bedeutung des autoradiographischen Verfahrens bei der Verwendung von kurzlebenden künstlichen radioaktiven Isotopen (Autoorganographie, Auto-angiographie). Experientia (Basel) 4, 406–407 (1948).

    Article  Google Scholar 

  • — Autoradiographies humaines (Automammagraphies, Autohépatographies, Autoangiographies, etc.) obtenues à l’aide d’un isotope radioactif artificiel (Z63). J. Radiol. Électrol. 30, 290–293 (1949).

    Google Scholar 

  • Nadler, N. J.: Some theoretical aspects of radioautography. Canad. J. med. Sci. 29, 182–194 (1951).

    CAS  Google Scholar 

  • — The quantitative estimation of radioactive isotopes by radioautography. Amer. J. Roentgenol. 70, 814–823 (1953).

    PubMed  CAS  Google Scholar 

  • Niklas, A., u. W. Maurer: Autoradiographie, in HOPPE-SEYLER-THIERFELDER, „Handbuch der physiologisch-chemischen Analyse“, Band II, 10. Aufl. Berlin-Göttingen-Heidelberg: Springer 1955.

    Google Scholar 

  • W. Oehlert u. Roesch: Autoradiographische Untersuchung der Größe des Eiweißstoff-wechsels verschiedener Organe, Gewebe und Zellarten. Beitr. path. Anat. 116, 92–123 (1956).

    CAS  Google Scholar 

  • Odeblad, E.: Contributions to the theory and technique of quantitative autoradiography with P32 with special reference to the granulosa tissue of the Graafian follicles in the rabbit. Acta radiol. (Stockh.) Suppl. 93 (1951).

    Google Scholar 

  • — Artefacts in autoradiography. Acta radiol. (Stockh.) 39, 192–204 (1953).

    Article  CAS  Google Scholar 

  • — and C. A. Tobias: Autoradiography after activation of histological sections with deuterons. Arch. Biochem. Biophys. 49, 452 (1954).

    Article  PubMed  Google Scholar 

  • Oehlert, W., B. Schultze U. W. Maurer: Autoradiographische Untersuchung der Größe des Eiweißstoffwechsels der verschiedenen Zellen des Zentralnervensystems. Beitr. path. Anat. 119, 343–376 (1958).

    CAS  Google Scholar 

  • Pelc, S. R.: Radiation dose in tracer experiments involving autoradiography, CIBA Foundation Conference on Isotopes in Biochemistry, 138–151. Philadelphia: The Blakiston Co. 1951.

    Google Scholar 

  • — and A. Howard: Chromosome metabolism as shown by autoradiographs. Exp. Cell Res. Suppl. 2, 269–278 (1952).

    Google Scholar 

  • — A difference between spermatogonia and somatic tissues of mice in the incorporation of (8-14C)-adenine into deoxyribonucleic acid. Exp. Cell Res. 11, 128–134 (1956).

    Google Scholar 

  • Plaut, W., and D. Mazia: The distribution of newly synthesized DNA in mitotic division. J. Biophys. biochem. Cytol. 2, 573–587 (1956).

    Article  PubMed  CAS  Google Scholar 

  • Roofe, P. G., F. E. Hoecker and C. D. Voorhees: A rapid bone sectioning technic. Proc. Soc. exp. Biol. (N. Y.) 72, 619–621 (1949).

    Article  CAS  Google Scholar 

  • Rotblad, J., and G. Ward: Analysis of the radioactive content of tissues by α-track autoradiography. Physics in Med. a. Biol. 1, 57–70 (1956).

    Article  Google Scholar 

  • Schaefer, H.: Nachweis und Messung kleinster α-Aktivitäten in biologischen Substanzen durch Bahnspurauszählung in der Photoemulsion. Strahlenther. 77, 613–628 (1948).

    CAS  Google Scholar 

  • Schmeiser, K.: Autoradiographie, in „Künstliche radioaktive Isotope in Physiologie, Diagnostik und Therapie“. 1. Aufl., S. 76–92. Berlin-Göttingen-Heidelberg: Springer 1953.

    Chapter  Google Scholar 

  • Schneider, W. C.: Phosphorus compounds in animal tissues. I. Extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. J. biol. Chem. 161, 293–303 (1945).

    PubMed  CAS  Google Scholar 

  • Schultze, B., W. Oehlert u. W. Maurer: Autoradiographische Untersuchung zum Mechanismus der Eiweißneubildung in Ganglienzellen. Beitr. path. Anat. 120, 58–84 (1958).

    Google Scholar 

  • Siess, M., u. G. Seybold: Das Imprägnierungsverfahren der Autoradiographie von Gewebs-schnitten. Z. wiss. Mikrosk. 63, 156–170 (1957).

    PubMed  CAS  Google Scholar 

  • Simmel, E. B., P. J. Fitzgerald and J. T. Godwin: Staining of radioautographs with metanil yellow and iron hematoxylin. Stain Technol. 26, 25–28 (1951).

    PubMed  CAS  Google Scholar 

  • Sognnaes, R. F., J. H. Shaw, A. K. Solomon and E. Harvold: A method for radioautography of specimens composed of both hard and soft structures. Anat. Rec. 104, 319–329 (1949).

    Article  PubMed  CAS  Google Scholar 

  • Stevens, G. W. W.: Resolution testing in autoradiography. Nature (Lond.) 161, 432–433 (1948).

    Article  CAS  Google Scholar 

  • — Radioactive microphotographs for resolution testing in autoradiography. Brit. J. Radiol. 23, 723–730 (1950).

    Article  PubMed  CAS  Google Scholar 

  • Taugner, R., U. Wagenmann, H. Hole, G. Grigoleit, R. Droh u. J. Gillissen: Serienmäßige Herstellung von Gefrierschnitt-Autoradiogrammen mit optimalem Kontakt. Naunyn-Schmiedebergs. Arch. exp. Path. Pharmak. 234, 336–342 (1958).

    CAS  Google Scholar 

  • Tauxe, W. N., A. H. Moser and G. A. Boyd: Etymology of autoradiography. Science 120, 149–150 (1954).

    Article  PubMed  CAS  Google Scholar 

  • Taylor, J. H.: Autoradiographic detection of incorporation of P32 into chromosomes during meiosis and mitosis. Exp. Cell Res. 4, 164–173 (1953).

    Article  CAS  Google Scholar 

  • — Autoradiography at the cellular level. Physical Techniques in Biological Research, Vol. 3, Cells and Tissues. New York: Acad. Press 1956.

    Google Scholar 

  • — Sister chromatid exchanges in tritium-labeled chromosomes. Genetics 43, 515–529 (1958).

    PubMed  CAS  Google Scholar 

  • — and R. Mcmaster: Autoradiographic and microphotometric studies of deoxyribose nucleic acid during microgametogenesis in Lilium longiflorum. Chromosoma 6, 489–521 (1954).

    PubMed  Google Scholar 

  • P. S. Woods and W. L. Hughes: The organization and duplication of chromosomes as revealed by autoradiographic studies using Tritium-labeled thymidine. Proc. nat. Acad. Sci. (Wash.) 43, 122–128) (1957).

    Article  Google Scholar 

  • Webb, J. M.: The reaction of ionizing particles with the photographic emulsion to produce the latent image, Manual for autoradiographic course, Oak Ridge Institute of Nuclear Studies. Oak Ridge, Tenn. 1951.

    Google Scholar 

  • Williams, A. I.: A method for prevention of leaching and fogging in autoradiographs. Nucleonics 8, No. 6, 10–14 (1951).

    PubMed  CAS  Google Scholar 

  • Woodruff, L. A., and W. P. Norris: Sectioning of undecalcified bone. With special reference to radioautographic application. Stain Technol. 30, 179–188 (1955).

    PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

H. Schwiegk F. Turba

Rights and permissions

Reprints and permissions

Copyright information

© 1961 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Harbers, E. (1961). Autoradiographie. In: Schwiegk, H., Turba, F. (eds) Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie/Radioactive Isotopes in Physiology Diagnostics and Therapy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-92819-2_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-92819-2_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-49048-4

  • Online ISBN: 978-3-642-92819-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics