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Abstract

With the increasing use of tritium as a radioactive tracer for biochemical studies, the availability of a simple and accurate method for the determination of this isotope in organic compounds and especially in biological materials is of considerable practical importance. Because of the low energy of the β-emanation from tritium disintegration (0.018 MEV), direct counting of thin films of tritiated material using end-window or gas-flow counters is not well suited for precise measurements.

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References

  1. 1.
    Bell, C. G., Jr., and F. N. Hayes, ED.: Liquid scintillation counting. New York-London-Paris: Pergamon Press 1958.Google Scholar
  2. 2.
    Chen, P. S., Jr.: Liquid scintillation counting of C14 and H3 in plasma and serum. Proc. Soc. exp. Biol. (N. Y.) 98, 546 (1958).CrossRefGoogle Scholar
  3. 3.
    Davidson, J. D., and P. Feigelson: Practical aspects of internal-sample liquid scintillation counting. Int. J. appl. Rad. and Isotopes 2, 1 (1957).CrossRefGoogle Scholar
  4. 4.
    Glascock, R. F.: Isotopic gas analysis for biochemists. Ch. 5, 8, 9. New York, N. Y.: Academic Press, Inc. 1954.Google Scholar
  5. 5.
    Hayes, F. N., and R. G. Gould: Liquid scintillation counting of tritium-labeled water and organic compounds. Science 117, 480 (1953).PubMedCrossRefGoogle Scholar
  6. 6.
    Henriques, F. C., Jr., and C. Margnetti: Analytical procedure for measurement of radioactive hydrogen. Indust. Engng. Chem., Anal. Ed. 18, 420 (1946).CrossRefGoogle Scholar
  7. 7.
    Herberg, R. J.: Phosphorescence in liquid scintillation counting of proteins. Science 128, 199 (1958).PubMedCrossRefGoogle Scholar
  8. 8.
    Jacobson, H. I., G. Gupta, C. Fernandez, S. Hennix and E. v. Jensen: Determination of tritium in biological material. Arch. Biochem. Biophys. 86, 89 (1960).PubMedCrossRefGoogle Scholar
  9. 9.
    Kamen, M. D.: Radioactive tracers in biology. 3rd ed. p. 276ff. New York, N.Y.: Academic Press, Inc. 1957.Google Scholar
  10. 10.
    Okita, G. T.: Personal communication.Google Scholar
  11. 11.
    Sinex, F. M., et al: Tritium tracing-A rediscovery. Nucleonics 16, No. 3, 62 (1958).Google Scholar
  12. 12.
    Vaughan, M., D. Steinberg and J. Logan: Liquid scintillation counting of C14-and H3- labeled amino acids and proteins. Science 126, 446 (1957).PubMedCrossRefGoogle Scholar
  13. 13.
    White, C. G., and S. Helf: Suspension counting in scintillating gels. Nucleonics 14, No. 10, 46 (1956).Google Scholar
  14. 14.
    White, D. F., I. G. Campbell and P. R. Payne: Estimation of radioactive hydrogen. Nature (Lond.) 166, 628 (1950).CrossRefGoogle Scholar
  15. 15.
    Wilzbach, K. E., L. Kaplan and W. G. Brown: Preparation of gas for the assay of tritium in organic compounds. Science 118, 522 (1953).PubMedCrossRefGoogle Scholar
  16. 16.
    — and W. Y. Sykes: Determination of isotopie carbon in organic compounds. Science 120, 494 (1954).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1961

Authors and Affiliations

  • Elwood von Jensen

There are no affiliations available

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