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Stereology pp 189-190 | Cite as

Determination of the Beta Granule Mass in Pancreatic Islets Using Linear Scanning Methods

  • A. M. Carpenter
  • A. Lazarow

Abstract

Linear scanning technics were used to quantitate islets of normal, alloxan subdiabetic and alloxan diabetic animals. At low magnification (light microscope), the islet volume was determined on aldehyde-fuchsin stained sections. With oil immersion objectives, the volumes of the cellular components of the islets were obtained. In addition, by scanning electron micrographs, the volume of beta granules quantitated was expressed as per cent of beta cell cytoplasm. Using the successive values, beta granule “mass” (% beta granules x % beta cells x % islet volume) are expressed as per cent of pancreatic volume. These data are correlated with estimates of insulin expressed as units per gram of pancreas.

Keywords

Beta Cell Pancreatic Islet Scan Electron Micrographs Insulin Content Scan Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer-Verlag New York Inc. 1967

Authors and Affiliations

  • A. M. Carpenter
    • 1
  • A. Lazarow
    • 1
  1. 1.Department of AnatomyUniversity of MinnesotaMinneapolisUSA

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