Advertisement

Red Cell Shape pp 131-140 | Cite as

Image Holograms of Single Red Blood Cell Discocyte-Spheroechinocyte Transformations

  • E. A. Evans
  • P. F. Leblond
Conference paper

Abstract

The mammalian red blood cell exhibits many morphological states in accordance with different physical and chemical environments. The spectrum of the erythrocyte shape ranges from a discocyte to a spherocyte; among the intermediate states are spiculated varieties known as echinocytes (from the greek: sea urchin) of different degrees. The reversible shape change from a biconcave disk into a crenated sphere, first described in 1895 by Hamburger [13], still remains an unresolved puzzle for many a hematologist and cell physiologist. Despite a wealth of valuable information derived mostly from the work of Ponder [14], investigators are still actively searching for a common underlying mechanism which could explain in a satisfactory manner the fact that a multiplicity of agents or conditions are call capable of inducing this transformation. The mechanics of the shape transformation are specified by the geometrical states and the physical forces involved. The physical forces result from the membrane or interfacial elastic and surface tractions, electrical forces due to induced and bound surface charges, osmotic forces and fluid hydrostatic pressure.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Barer (R.) and Joseph (S.) (1954–1955): Refractometry of living cells. Part I: Basic principles; Part II: The immersion medium. Quart. Journ. Microscop. Science, 95, 339 and 96, 1.Google Scholar
  2. 2.
    Bessis (M.) and Lessin (L. S.) (1970): The discocyte-echinocyte equilibrium of the normal and pathologic red cell. Blood, 36, 399.PubMedGoogle Scholar
  3. 3.
    Bessis (M.) et Prenant (M.) (1972): Topographie de l’apparition des spicules dans les érythrocytes crénelés (échinocytes). Nouv. Rev. Franç Hématol., 12, 351.Google Scholar
  4. 4.
    Braasch (D.) (1969): On the relation between erythrocyte deformability, cell shape and membrane surface tension. Arch. Gesel. Physiol., 313, 316.CrossRefGoogle Scholar
  5. 5.
    Brecher (G.) and Bessis (M.) (1972): Present status of spiculated red cells and their relationship to the discocyte-echinocyte transformation. A critical review. Blood, 40, 333.PubMedGoogle Scholar
  6. 6.
    Canham (P. B.) (1969): Difference in geometry of young and old human erythrocytes explained in a filtering mechanism. Circ. Res., 25, 39.PubMedGoogle Scholar
  7. 7.
    Canham (P. B.) and Burton (A. C.) (1968): Distribution of size and shape in populations of normal human red cells. Circ. Res., 22, 405.PubMedGoogle Scholar
  8. 8.
    Deuticke (B.) (1968): Transformation and restauration of biconcave shape of human erythrocytes induced by amphiphilic agents and changes of ionic environment. Biophys. Biochim. Acta, 163, 494.CrossRefGoogle Scholar
  9. 9.
    Evans (E. A.) (1971): Quantitative reconstruction and superresolution of red blood cell image holograms. Journ. Opt. Soc. Amer., 61, 991.CrossRefGoogle Scholar
  10. 10.
    Evans (E. A.) (1970): Comparison of the diffraction theory of image formation with the three-dimensional, first born scattering approximation in lens systems. Optics Communications, 2, 317.CrossRefGoogle Scholar
  11. 11.
    Evans (E. A.) and Fung (Y. C. B.) (1972): Improved measurements of the erythrocyte geometry. Microvasc. Res. (in press).Google Scholar
  12. 12.
    Evans (E. A.) and Leblond (P. F.) (1972): Geometric properties of individual red blood cell discocyte-spherocyte transformations. Biorheology (submitted for publication).Google Scholar
  13. 13.
    Hamburger (H. J.) (1895): Pfluger’s Archives, 141, 230, cited in Ponder (E.) (1971). Ref. 14.Google Scholar
  14. 14.
    Ponder (E.) (1971): Hemolysis and related phenomena. Reissued edition. Grune and Stratton, New York.Google Scholar
  15. 15.
    Rand (R. P.) and Burton (A. C.) (1964): Mechanical properties of the red cell membrane. I. Mechanical stiffness and intracellular pressure. Biophys. Journ., 4, 115.CrossRefGoogle Scholar
  16. 16.
    Rand (R. P.), Burton (A. G.) and Canham (P. B.) (1965): Reversible changes in shape of red cells in electrical fields. Nature, 205, 977.CrossRefGoogle Scholar
  17. 17.
    Weed (R. I.), La Celle (P. L.) and Merrill (E. W.) (1969): Metabolic dependence of red cell deformability. Journ. Clin. Invest., 48, 795.CrossRefGoogle Scholar

Copyright information

© Masson & Cie, Editeurs, Paris 1973

Authors and Affiliations

  • E. A. Evans
    • 1
  • P. F. Leblond
    • 1
  1. 1.Institut de Pathologie CellulaireHôpital de BicêtreLe Kremlin-BicêtreFrance

Personalised recommendations