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Carboxypeptidase Inhibitor from Potatoes, Properties and Amino Acid Sequence

  • C. A. Ryan
  • G. M. Hass
  • R. W. Kuhn
  • H. Neurath
Part of the Bayer-Symposium book series (BAYER-SYMP, volume 5)

Abstract

The initial report [1] of the existence of a naturally occurring carboxypeptidase inhibitor in potatoes originated from the observation that crystalline preparations of potato inhibitor I possessed inhibitory activity against carboxypeptidase B. Subsequently, it was discovered, that the carboxypeptidase inhibitor activity was an impurity in the crystals [2]. The inhibitor, herein called CP-I4, eluted from Sephadex G-75 with an apparent molecular weight of about 4000. It was also found to inhibit carboxypeptidase A even more potently than carboxypeptidase B [3], but did not inhibit the endopeptidases chymotrypsin and trypsin.

Keywords

Russet Burbank Potato Edman Degradation Pseudomonas Stutzeri Automatic Edman Degradation Bond Splitting 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • C. A. Ryan
    • 1
    • 2
  • G. M. Hass
    • 3
    • 4
  • R. W. Kuhn
    • 3
    • 5
  • H. Neurath
    • 3
  1. 1.Department of Agricultural ChemistryWashington State UniversityPullmanUSA
  2. 2.Career Development Awardee of the U.S. Public Health ServiceUSA
  3. 3.Department of BiochemistryUniversity of WashingtonSeattleUSA
  4. 4.Investigator of the Howard Hughes Medical InstituteUSA
  5. 5.Department of Cell BiologyBaylor University Medical CenterHoustonUSA

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