Replacements, Insertions, and Modifications of Amino Acid Residues in the Reactive Site of Soybean Trypsin Inhibitor (Kunitz)

  • D. Kowalski
  • T. R. Leary
  • R. E. McKee
  • R. W. Sealock
  • D. Wang
  • M. LaskowskiJr.
Part of the Bayer-Symposium book series (BAYER-SYMP, volume 5)

Abstract

In the further attempt to understand the molecular details of the inhibition of proteolytic enzymes by protein inhibitors, our laboratory has undertaken an extensive study of the effects of alterations of specific reactive site amino acid residues on the enzyme-inhibitor interaction. Removals, replacements and insertions of amino acid residues in the reactive site of the soybean trypsin inhibitor (STI) of Kunitz have been carried out as well as chemical modifications of functional groups.

Keywords

Titration Carbonyl Trypsin Disulfide Anhydride 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ozawa, K., Laskowski, M., Jr.: J. biol. Chem. 241, 3955 (1966).PubMedGoogle Scholar
  2. 2.
    Koide, T., Tsunasawa, S., Ikenaka, T.: J. Biochem. (Tokyo) 71, 165 (1972).Google Scholar
  3. 3.
    Schechter, I., Berger, A.: Biochem. Biophys. Res. Commun. 27, 157 (1967).PubMedCrossRefGoogle Scholar
  4. 4.
    Finkenstadt, W.R., Laskowski, M., Jr.: J. biol. Chem. 240, PC 962 (1965).Google Scholar
  5. 5.
    Sealock, R. W., Laskowski, M., Jr.: Biochemistry 8, 3703 (1969).PubMedCrossRefGoogle Scholar
  6. 6.
    Finkenstadt, W.R., Laskowski, M., Jr.: J. biol. Chem. 242, 771 (1967).PubMedGoogle Scholar
  7. 7.
    Sealock, R. W.: Ph. D. Thesis, Purdue University, 1972.Google Scholar
  8. 8.
    Niekamp, C. W.: Ph. D. Thesis, Purdue University, 1971.Google Scholar
  9. 9.
    Blow, D. M.: personal communication.Google Scholar
  10. 10.
    Krahn, J., STEVENS, F.C: Biochemistry 11, 1804 (1972).PubMedCrossRefGoogle Scholar
  11. 11.
    Stevens, F.C., Wuerz, ST., Krahn, J.: This volume, p. 344.Google Scholar
  12. 12.
    Odani, S., Ikenaka, T.: J. Biochem. (Tokyo) 71. 839 (1972).PubMedGoogle Scholar
  13. 13.
    Ikenaka, T.. Odam.S., Koide, T.: This volume, p. 325.Google Scholar
  14. 14.
    Seidl, D.S., Liener, I.E.: Biochim. biophys. Acta (Amst.) 251, 831 (1971).Google Scholar
  15. 15.
    Seidl, D.S., Liener, I.E.: Biochim. biophys. Acta (Amst.) 258, 303 (1972).Google Scholar
  16. 16.
    Leary, T.R., Laskowski, M., Jr.: Biochemistry (to be submitted).Google Scholar
  17. 17.
    Schiessler, H., Arnhold, M., Fritz, H.: This volume, p. 147.Google Scholar
  18. 18.
    Iwasaki, T., Kiyohara, T., Yoshikawa, M.: J. Biochem. (Tokyo) 73, 1039 (1973).Google Scholar
  19. 19.
    Iwasaki, T., Kiyohara, T., Yoshikawa, M.: J. Biochem. (Tokyo) 71, 335 (1973).Google Scholar
  20. 20.
    Robertus, J.D., Kraut, J., Alden, R.A., Birktoft, J.J.: Biochemistry 11, 4293 (1972).PubMedCrossRefGoogle Scholar
  21. 21.
    Laskowski, M., Jr., Duran, R.W., Finkenstadt, W.R., Herbert, S., Hixson, H.F., Jr., Kowalski, D., Luthy, J.A., Mattis, J.A., Mckee, R.E., Niekamp, C.W.: In: Fritz, H., Tschesche, H. (Eds.): Proceedings of the International Research Conference on Protei-nase Inhibitors, p. 117. Berlin-New York: Walter de Gruyter 1971.Google Scholar
  22. 22.
    Mckee, R.E., Niekamp, C. W., Laskowski, M., Jr.: Abstracts 162nd National Meeting of the American Chemical Society, Washington, D. C., 1971, Biol No.233.Google Scholar
  23. 23.
    Kowalski, D., Laskowski, M., Jr.: Biochemistry 11, 3451 (1972).PubMedCrossRefGoogle Scholar
  24. 24.
    Steiner, R.F.: Arch. Biochem. Biophys. 115, 257 (1966).PubMedCrossRefGoogle Scholar
  25. 25.
    Rlhlmaw, A.. Kukla. D., Schwager, P. Bartels. K., Hiber. R.: J. molec. Biol. 77. 417 (1973).CrossRefGoogle Scholar
  26. 26.
    Et., Janin. J. Blow. D. M.: This volume, p. 513.Google Scholar
  27. 27.
    Dobry. A.. Fruton, J.S., Sturtevant. J.M.: J. biol. Chem. 195, 149 (1952).PubMedGoogle Scholar
  28. 28.
    Kowalski, D.: Ph. D. Thesis, Purdue University. 1974.Google Scholar
  29. 29.
    Wang, D., Laskowski, M., Jr.: Abstracts 164th National Meeting of the American Chemical Society, New York, N.Y., 1972, Biol No. 134.Google Scholar
  30. 30.
    Wang, D., Laskowski, M., Jr.: to be published.Google Scholar
  31. 31.
    Finkenstadt, W.R., Hamid, M. A., Mattis, J.A., Schrode, J., Sealock, R. W., Wang, D., Laskowski, M., Jr.: This volume, p. 389.Google Scholar
  32. 32.
    Niekamp, C. W., Hixson, H. F., Jr., Laskowski, M, Jr.: Biochemistry 8, 16 (1969).PubMedCrossRefGoogle Scholar
  33. 33.
    Laskowski, M., Jr.: In: Fritz, H., Tschesche, H. (Eds.): Proceedings of the International Research Conference on Proteinase Inhibitors, p. 64. Berlin-New York: Walter de Gruyter 1971.Google Scholar
  34. 34.
    Rigbi, M., Greene, L.J.: J. biol. Chem. 243, 5457 (1968).PubMedGoogle Scholar
  35. 35.
    Hochstrasser, K., Schramm, W., Fritz, H., Schwarz, S., Werle, E.: Z. physiol. Chem. 350, 893 (1968).CrossRefGoogle Scholar
  36. 36.
    Tschesche, H., Wachter, E.: Europ. J. Biochem. 16, 187 (1970).PubMedCrossRefGoogle Scholar
  37. 37.
    Tschesche, H., Wachter, E., Kupfer, S., Obermeier, R., Reidel, G., Haenisch, G., Schneider, M.: In: Fritz, H., Tschesche, H. (Eds.): Proceedings of the International Research Conference on Proteinase Inhibitors, p.207. Berlin-New York: Walter de Gruyter 1971.Google Scholar
  38. 38.
    Greene, L.J., Bartelt, D.C.: This volume, p. 188.Google Scholar
  39. 39.
    Wilson, K. A., Laskowski, M., Sr.: This volume, p. 286.Google Scholar
  40. 40.
    Hochstrasser, K., Illchmann, K., Werle, E.: Z. physiol. Chem. 351, 1503 (1970).CrossRefGoogle Scholar
  41. 41.
    Belitz, H.D., Fuchs, A.: Z. Lebensm.-Unters. Forsch. 152, 129 (1973).CrossRefGoogle Scholar
  42. 42.
    Huber, R., Kukla, D., Rlhlmann, A., Steigemann, W.: In: Fritz, H., Tschesche, H. (Eds.): Proceedings of the International Research Conference on Proteinase Inhibitors, p.56. Berlin-New York: Walter de Gruyter 1971.Google Scholar
  43. 43.
    Fersht, A.R., Blow, D.M., Fastrez, J.: Biochemistry 12, 2035 (1973).PubMedCrossRefGoogle Scholar
  44. 44.
    Laskowski, M., Jr., Kato, I., Leary, T. R., Schrode, J., Sealock,R. W.: This volume, p. 597.Google Scholar
  45. 45.
    Takahashi, H., Iwanaga, S., Kitagawa, T., Suzuki, T.: This volume, p. 265.Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • D. Kowalski
    • 1
  • T. R. Leary
    • 1
  • R. E. McKee
    • 1
  • R. W. Sealock
    • 1
  • D. Wang
    • 1
  • M. LaskowskiJr.
    • 1
  1. 1.Department of ChemistryPurdue UniversityW. LafayetteUSA

Personalised recommendations