The Partial Linear Sequence of Garden Bean Inhibitor II and Location of the Protease Reactive Sites

Abstract

We have recently reported the isolation of three isoinhibitors of trypsin from the garden bean, Phaseolus vulgaris, var. Great Northern [1]. These inhibitors closely resemble the other low molecular weight legume inhibitors from lima beans [2, 3], mung bean [4], navy and kidney beans [5, 6], and the Bowman-Birk inhibitor of soybean [7]. They have in common a low molecular weight (8000 to 10000), high contents of serine, aspartic acid, and half-cystine and the absence of tryptophan and carbohydrate.

Supported by Grant HL-15892 from the National Institutes of Health and Grant PRP-30 from the American Cancer Society. During early portions of this work K.A.W. was a Predoctoral Fellow of the National Science Foundation.