Molecular Characterization of Transformed Plants
The generation of genetically transformed plants is central to, and has indeed revolutionized, plant molecular biology. This is true for studies at both the fundamental and more applied levels of research. For researchers interested in unravelling the roles of specific genes in particular pathways of growth and development, the introduction into plants of foreign genes and gene promoters linked to reporter genes allows the detailed study of the temporal, spatial and quantitative expression of plant genes and the activities of associated regulatory sequences. In our own laboratory, we use these techniques in a programme of insertional mutagenesis to identify developmentally interesting genes (Topping et al. 1991; Lindsey et al. 1993; Topping et al. 1994). In the more applied area of genetic engineering, which is directed towards crop improvement, the introduction of novel genes encoding, for example, resistance to various pests and herbicides into economically important species, is in the long term likely to develop into a major branch of the plant breeding industry (Lindsey 1992). There are several well-characterized and very successful methods which are currently being employed to introduce specific genes and gene regulatory sequences into plants and these are described in Chapter 8 of this book.
KeywordsSelectable Marker Gene Plant Molecular Biology Crude Cell Extract Microprojectile Bombardment Sterile Double Distil Water
Unable to display preview. Download preview PDF.
- Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: ß-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3301–3307Google Scholar
- Nagy F, Odell JT, Morelli G, Chua N-H (1985) Properties of expression of the 35S promoter from CaMV in transgenic tobacco plants. In: Zaitlin M, Day P, Hollaender A (eds) Biotechnology in plant science: relevance to agriculture in the eighties. Academic Press, Orlando, pp 227–235CrossRefGoogle Scholar
- Reynaerts A, De Block M, Hernalsteens J-P, van Montagu M (1988) Selectable and screenable markers. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual. Kluwer, Dordrecht A9.T-16Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Molecular cloning: a laboratory manual 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Stomp A-M (1990) Use of X-Gluc for histochemical localisation of glucuronidase. In: Editorial comments. United State Biochemical, Cleveland, p 5Google Scholar