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Cloning from Genomic DNA and Production of Libraries

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Plant Molecular Biology — A Laboratory Manual

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Abstract

Polymerase Chain Reaction (PCR) is an in vitro method of nucleic acid synthesis by which a particular segment of DNA can be specifically replicated. The technique is an ingenious tool for molecular biology that has had more impact on research than the discovery of restriction enzymes and Southern blotting. Since the unveiling of the method by Kary Mullis and colleagues (Saiki et al. 1985, 1988), numerous modifications, improvements and novel applications of PCR have been devised; since then more than 1000 publications involving the use of this method have appeared in the scientific literature.

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Authors
  1. A. Guevara-Garcia
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  2. L. Herrera-Estrella
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  3. G. Olmedo-Alvarez
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  4. M. S. Clark
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  5. G. Elgar
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  6. C.-N. Liu
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  7. E. Matallana
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  8. J. Simpson
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  9. P. A. Guzman
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Editor information

Editors and Affiliations

  1. Molecular Genetics Unit, School of Clinical Medicine, Department of Medicine, Level 5, Addenbrooke’s Hospital, University of Cambridge, Hills Road, Cambridge, CB2 2QQ, UK

    Melody S. Clark

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© 1997 Springer-Verlag Berlin Heidelberg

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Guevara-Garcia, A. et al. (1997). Cloning from Genomic DNA and Production of Libraries. In: Clark, M.S. (eds) Plant Molecular Biology — A Laboratory Manual. Springer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87873-2_2

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  • DOI: https://doi.org/10.1007/978-3-642-87873-2_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-49144-3

  • Online ISBN: 978-3-642-87873-2

  • eBook Packages: Springer Book Archive

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