P-BR and Its Role in the Photocycle of Bacteriorhodopsin
The initial photochemical events occuring in the chromophore (bacteriorhodopsin) of the purple membrane of Halobacterium halobium belongs to one of the most studied subjects in the literature of picosecond spectroscopy. Both fast fluorescence [1–3] and absorption recovery [4–7] has been studied. However, there is rather poor agreement between experimental data reported. In some recent flash photolysis  and fluorescence studies [9–10] it has been shown that there is a long-lived photoproduct of bacteriorhodopsin, called P-BR, which is formed even at 77 K. We have undertaken this study in order to investigate if some of the results given in the literature might be better understood assuming that P-BR contributes to the data reported.
KeywordsPurple Membrane Critical Flow Rate Fast Fluorescence Protonated Schiff Base Halobacterium Halobium
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