Abstract
Synthetic oligonucleotides are useful research tools in molecular biology [3, 7]. As hybridization probes, they can distinguish a perfect match in the target sequences from various single-base-pair mismatches [20, 21]. They have been used to introduce mutations in DNA at specific sites [8, 13, 15, 22, 23] as well as to probe for mutations at specific loci in genes [4, 9, 13, 20, 22, 23]. They have also been employed in the isolation of specific DNA sequences from a bank of cloned cDNA [6, 16, 19, 21] and in the determination of RNA secondary structure [11]. Moreover, the use of antisense DNA or RNA oligonucleotides to block the translation or processing of specific mRNAs provides a powerful tool for studies of the regulation of gene expression and development [2, 5].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Causeret C, Bentéjac M, Bugaut M (1993) Proteins and enzymes of the peroxisomal membrane in mammals. Biol Cell 77:89–104
Cohen JS (1991) Antisense oligodeoxynucleotides as antiviral agents. Antiviral Res 16:121–133
Eckstein F (1991) Oligonucleotides and analogues: A practical approach, IRL Press, Oxford
Farr CJ, Saiki RK, Erlich HA, McCormick F, Marshall CJ (1988) Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes. Proc Natl Acad Sci USA 85:1629–1633
Hélène C, Toulmé JJ (1990) Specific regulation of gene expression by antisense, sense and antigene nucleic acids. Biochim Biophys Acta 1049:99–125
Heller RA, Song K, Freire-Moar J (1992) Rapid screening of libraries with radiolabeled DNA sequences generated by PCR using highly degenerate oligonucleotide mixtures. Biotechniques 12:30–35
Itakura K, Rossi JJ, Wallace RB (1984) Synthesis and use of synthetic oligonucleotides. Annu Rev Biochem 53:323–356
Ito W, Ishiguro H, Kurosawa Y (1991) A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction. Gene 102:67–70
Karnik SS, Khorana GH (1990) Assembly of functional rhodopsin requires a disulfure bond between cysteine residues 110 and 187. J Biol Chem 265:17520–17524
Latruffe N (1992) Les peroxysomes et la proliferation cellulaire ou la prise en considération d’un organite méconnu. Médecine/Sciences 8:239–248
McWilliams RA, Glitz DG (1991) Localization of a segment of 16S RNA on the surface of the small ribosomal subunit by immune electron microscopy of complementary oligodeoxynucleotides. Biochimie 73:911–918
Miyazawa S, Hayashi H, Hijikata M, Ishii N, Furuta S, Kagamiyamla H, Osumi T, Hashimoto T (1987) Complete nucleotide sequence of cDNA and predicted aminoacide sequence of rat acyl-CoA oxidase. J Biol Chem 262:8131–8137
Norrander J, Kempe T, Messing J (1983) Construction of improved M13 vectors using oligodeoxynucleo tide-directed mutagenesis. Gene 26:101–106
Osumi T, Ozasa H, Miyazawa S, Hashimoto T (1984) Molecular cloning of cDNA for rat liver catalase. Biochem Biophys Res Commun 122:831–837
Sayers JR, Krekel C, Eckstein F (1992) Rapid high-efficiency site-directed mutagenesis by the phosphorothioate approach. Biotechniques 13:592–596
Suggs SV, Wallace BR, Hirose T, Kawashima EH, Itakura K (1981) Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human β2-microglobulin. Proc Natl Acad Sci USA 78:6613–6617
Suzuki H, Kawarabayasi Y, Kondo J, Abe T, Nishikawa K, Kimura S, Hashimoto T, Yamamoto T (1990) Structure and regulation of rat long-chain acyl-CoA synthetase. J Biol Chem 265:8681–8685
Van Den Bosch H, Schutgens RBH, Wanders RJA, Tager JM (1992) Biochemistry of peroxisomes. Annu Rev Biochem 61:157–197
Wallace RB, Johnson MJ, Hirose T, Miyake T, Kawashima EH, Itakura K (1981) The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA. Nucl Acids Res 9:879–894
Wallace RB, Shaffer J, Bonner J, Hirose T, Itakura K (1979) Hybridization of synthetic oligodeoxyribonucleotides to φ χ 174 DNA: the effect of single base pair mismatch. Nucl Acids Res 6:3543–3557
Wood WI, Gitschier J, Lasky LA, Lawn RM (1985) Base composition-independent hybridization in tetramethylammonium chloride: A method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci USA 82:1585–1588
Zoller MJ, Smith M (1982) Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucl Acids Res 10:6487–6500
Zoller MJ, Smith M (1983) Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. In Wu R, Grossman L and Moldave K (eds). Methods in Enzymology, Vol. 100, Academic, New York, pp. 468–500
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Bentéjac, M., Bugaut, M. (1994). Synthesis of Oligonucleotides Used as Probes; Purification by HPLC. In: Latruffe, N., Bugaut, M. (eds) Peroxisomes. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87807-7_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-87807-7_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-87809-1
Online ISBN: 978-3-642-87807-7
eBook Packages: Springer Book Archive