Abstract
The first three steps of ether-lipid biosynthesis are catalysed by enzymes located in the peroxisomal membrane, whereas the further steps are associated with the endoplasmic reticulum [4,9]. The peroxisomal enzymes are dihydroxyacetonephosphate (DHAP) acyltransferase, alkyl-DHAP synthase and alkyl-DHAP reductase. Dihydroxyacetonephosphate acyltransferase (DHAP-AT, E.C. 2.3.1.42) is found only in peroxisomes [3] and, therefore, can be used as an enzymatic marker of this organelle [8]. DHAP-AT’s active site is on the inner face of the membrane. The enzyme catalyses the first step in the synthesis of ether lipids, which have acyl-DHAP as an obligate precursor.
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© 1994 Springer-Verlag Berlin Heidelberg
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Causeret, C., Bentéjac, M., Bugaut, M. (1994). Measurement of Dihydroxyacetone-Phosphate Acyl-Transferase (DHAP-AT) Activity in Liver Peroxisomes and Cell Lines. In: Latruffe, N., Bugaut, M. (eds) Peroxisomes. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87807-7_13
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DOI: https://doi.org/10.1007/978-3-642-87807-7_13
Publisher Name: Springer, Berlin, Heidelberg
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