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Peroxisomes pp 151-157 | Cite as

Measurement of the in Vitro Glucuronidation of Peroxisome Proliferator Carboxylic Acids by Liver Microsomes and Genetically Modified V79 Cell Line

  • H. Goudonnet
  • J. Magdalou
  • S. Fournel-Gigleux
Part of the Springer Laboratory book series (SLM)

Abstract

Peroxisome proliferators (PP) belonging to the series of carboxylic acids are hypolipidemic drugs (clofibric acid, ciprofibrate, fenofibrate,...) or plastifiers (2-ethylhexanoic acid). In man, these substances are essentially excreted as acylglucuronides, which are formed by uridine-5’-diphosphate-(UDP)-glucuronosyltransferases (UGTs, EC 2.4.1.17), according to the following reaction: Open image in new window By contrast to ether glucuronides, ester (acyl) glucuronides are reactive metabolite species. At physiological pHs, they can undergo several non desirable reactions such as isomerization, hydrolysis, and transacylation, which can lead to the formation of covalently bound adducts with proteins [5]. This has been proposed as the mechanism which causes immunological reaction in patients receiving certain carboxylic acid drugs, other than fibrates, which has led to such drugs being withdrawn from the market. They are therefore toxicologically relevant.

Keywords

High Performance Liquid Chromatography High Performance Liquid Chromatography High Performance Liquid Chromatography Analysis Peroxisome Proliferators Clofibric Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • H. Goudonnet
  • J. Magdalou
  • S. Fournel-Gigleux

There are no affiliations available

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