Advertisement

Neurobiology pp 398-412 | Cite as

Two-Dimensional Gel Analysis of Polypeptides in Drosophila Compound Eyes

  • H. Matsumoto
  • W. L. Pak
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Several years ago, we undertook an analysis of Drosophila eye proteins in the hope of identifying eye-specific proteins that might be involved in molecular mechanisms of visual excitation. We chose Drosophila melanogaster as an experimental organism because of its well-known genetics and the availability of a large number of mutants defective in visual function (Pak 1975; Pak 1979; Hall 1982). The approach we took was to apply two-dimensional Polyacrylamide gel electrophoresis (2-D gel) to the analysis of proteins in the compound eye of both wild-type and mutant flies. Fujitaand Hotta (Fujita and Hotta 1979; Hotta 1979) first applied this technique to Drosophila eyes and showed that three polypeptides observed in 2-D gel originate from the compound eye. In addition to these three, we found at least three other polypeptides that are specific to the photoreceptor layer (Matsumoto et al. 1982). Moreover, the three polypeptides we identified (designated as 80 K, 49 K, and 39 K) were found to alter their isoelectric points in response to a light stimulus in vivo (Matsumoto et al. 1982).

Keywords

Photoreceptor Layer Phosphorylation Reaction Major Spot Photoreceptor Function Visual Excitation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Banga SS, Bloomquist BT, Brodberg RK, Pye QN, Larrivee DC, Mason JM, Boyd JB, Pak WL. Cytogenetic characterization of the 4BC region on the X chromosome of Drosophila melanogaster (in preparation)Google Scholar
  2. Braitenberg V (1967) Patterns of projection in the visual system of the fly. I. Retina-lamina projections. Exp Brain Res 3:271–298PubMedCrossRefGoogle Scholar
  3. Fujita S, Hotta Y (1979) Two-dimensional electrophoretic analysis of tissue specific proteins of Drosophila melanogaster. Protein, Nucl Acid Enzyme 24:1336–1343 (in Japanese)Google Scholar
  4. Hall JC (1982) Genetics of the nervous system in Drosophila. Q Rev Biophys 15:223–479PubMedCrossRefGoogle Scholar
  5. Heisenberg M (1971) Isolation of mutants lacking the optmotor response. Dros Inf Serv 46:68Google Scholar
  6. Hotta Y (1979) A biochemical analysis of visual mutations m Drosophila melanogaster. changes in major eye proteins. In: Ebert, Okada (eds) Mechanism of cell change. Wiley, New York, pp 169–182Google Scholar
  7. Hotta Y, Benzer S (1969) Abnormal electroretinograms in visual mutants oï Drosophila. Nature (Lond) 222:354–356CrossRefGoogle Scholar
  8. Laemmli LK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680–685CrossRefGoogle Scholar
  9. Lo M-V, Pak WL (1981) Light-induced pigment granule migration in the retinular cells of Drosophila melanogaster. J Gen Physiol 77:155–175PubMedCrossRefGoogle Scholar
  10. Matsumoto H, O’Tousa JE, Pak WL (1982) Light-induced modification of Drosophila retinal polypeptides in vivo. Science (Wash DC) 217:839–841CrossRefGoogle Scholar
  11. Matsumoto H, Isono K, Pak WL (1983) Light-induced phosphorylation of Drosophila retinal proteins. Invest Ophthalmol Visual Sci Suppl 24:115Google Scholar
  12. Matsumoto H, Pak WL (1984) Light-induced phosphorylation of retina-specific polypeptides of Drosophila in vivo. Science (Wash DC) 223:184–186CrossRefGoogle Scholar
  13. Miyazaki K, Hagiwara H, Yokota M, Kakuno T, Horio T (1978) Two-dimensional gel electrophoresis. In: Ui, Horio (eds) Isoelectric focusing and isotachophoresis. (in Japanese) Kyoritsu Shuppan, Tokyo, pp 183–196Google Scholar
  14. O’Farrell PM (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007–4021PubMedGoogle Scholar
  15. Pak WL, Grossfield J, White NV (1969) Nonphototactic mutants in a study of vision ofDrosophila. Nature (Lond) 222:351–354CrossRefGoogle Scholar
  16. Pak WL (1975) Mutants affecting the vision of Drosophila melanogaster. In: King RC (ed) Handbook of genetics, vol 3. Plenum, New York, pp 703–733Google Scholar
  17. Pak WL (1979) Study of photoreceptor function mmg Drosophila mutants. In: Breakefield XO (ed) Neurogenetics: genetic approaches to the nervous system. Elsevier, North HollandGoogle Scholar
  18. Trujillo-Cenóz O, Melamed J (1966) Compound eye of dipterans: anatomical basis for integration-an electron microscope study. J Ultrastruct Res 16:395–398PubMedCrossRefGoogle Scholar
  19. Weiler M (1979) Protein phosphorylation. Pion, LondonGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • H. Matsumoto
  • W. L. Pak
    • 1
  1. 1.Department of Biological SciencesPurdue UniversityWest LafayetteUSA

Personalised recommendations