Two-Dimensional Gel Analysis of Polypeptides in Drosophila Compound Eyes
Several years ago, we undertook an analysis of Drosophila eye proteins in the hope of identifying eye-specific proteins that might be involved in molecular mechanisms of visual excitation. We chose Drosophila melanogaster as an experimental organism because of its well-known genetics and the availability of a large number of mutants defective in visual function (Pak 1975; Pak 1979; Hall 1982). The approach we took was to apply two-dimensional Polyacrylamide gel electrophoresis (2-D gel) to the analysis of proteins in the compound eye of both wild-type and mutant flies. Fujitaand Hotta (Fujita and Hotta 1979; Hotta 1979) first applied this technique to Drosophila eyes and showed that three polypeptides observed in 2-D gel originate from the compound eye. In addition to these three, we found at least three other polypeptides that are specific to the photoreceptor layer (Matsumoto et al. 1982). Moreover, the three polypeptides we identified (designated as 80 K, 49 K, and 39 K) were found to alter their isoelectric points in response to a light stimulus in vivo (Matsumoto et al. 1982).
KeywordsMigration Acetone Albumin Recombination Amide
Unable to display preview. Download preview PDF.
- Banga SS, Bloomquist BT, Brodberg RK, Pye QN, Larrivee DC, Mason JM, Boyd JB, Pak WL. Cytogenetic characterization of the 4BC region on the X chromosome of Drosophila melanogaster (in preparation)Google Scholar
- Fujita S, Hotta Y (1979) Two-dimensional electrophoretic analysis of tissue specific proteins of Drosophila melanogaster. Protein, Nucl Acid Enzyme 24:1336–1343 (in Japanese)Google Scholar
- Heisenberg M (1971) Isolation of mutants lacking the optmotor response. Dros Inf Serv 46:68Google Scholar
- Hotta Y (1979) A biochemical analysis of visual mutations m Drosophila melanogaster. changes in major eye proteins. In: Ebert, Okada (eds) Mechanism of cell change. Wiley, New York, pp 169–182Google Scholar
- Matsumoto H, Isono K, Pak WL (1983) Light-induced phosphorylation of Drosophila retinal proteins. Invest Ophthalmol Visual Sci Suppl 24:115Google Scholar
- Miyazaki K, Hagiwara H, Yokota M, Kakuno T, Horio T (1978) Two-dimensional gel electrophoresis. In: Ui, Horio (eds) Isoelectric focusing and isotachophoresis. (in Japanese) Kyoritsu Shuppan, Tokyo, pp 183–196Google Scholar
- Pak WL (1975) Mutants affecting the vision of Drosophila melanogaster. In: King RC (ed) Handbook of genetics, vol 3. Plenum, New York, pp 703–733Google Scholar
- Pak WL (1979) Study of photoreceptor function mmg Drosophila mutants. In: Breakefield XO (ed) Neurogenetics: genetic approaches to the nervous system. Elsevier, North HollandGoogle Scholar
- Weiler M (1979) Protein phosphorylation. Pion, LondonGoogle Scholar