Distribution of Thymidilate Incorporation into the DNA of X 174-Infected Triphosphate Permeable Cells

  • H. Hoffmann-Berling
Conference paper

Abstract

Investigation of the replication of bacteriophage øX174 (øX) has shown that infecting single stranded DNA enters a double stranded replicative form (RF). This molecule undergoes repeated semiconser-vative replication and further RF appears (Sinsheimer, Starman, Nagler, and Guthrie, 1962). In an infected culture RF-DNA increases at a constant rate, and it has been suggested that only one RF-molecule in a cell, probably that containing the parental DNA-strand, provides the template for the synthesis of further RF (Denhardt and Sinsheimer, 1965 b). At a rate of 1–2 RF-molecules synthesized per cell per minute labeled DNA-precursors flow rapidly through replicating DNA into mature RF (Lindqvist and Sinsheimer, 1967) which according to Denhardt and Sinsheimer needs not replicate further. Preferential labeling of øX-DNA molecules engaged in the process of replication accordingly is difficult.

Keywords

Sucrose Magnesium Phenol EDTA Sedimentation 

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1968

Authors and Affiliations

  • H. Hoffmann-Berling
    • 1
  1. 1.Max-Planck-Institut für Medizinische ForschungHeidelbergDeutschland

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