Mutants of Serratia Marcescens Defective or Superactive in the Release of a Nuclease

  • U. Winkler


During growth several microorganisms, belonging to different systematic groups, release nucleases into the medium. One of these is Serratia marcescens (Jeffries et al., 1957; Rothberg and Swartz, 1965). Bacteria of this species presumably excrete only a single nuclease which degrades both, DNA and RNA, as revealed by biochemical studies (Eaves and Jeffries, 1963). Since extracellular nucleases are expected to be troublesome in certain experiments, e.g. transfections, we were interested in mutants of S. marcescens showing no more extracellular nucleolytic activity. While isolating these socalled nuc - mutants we found accidentally some others (nuc su ) which were superactive in excreting nuclease. This paper describes some of the properties of these nuc - and nuc su mutants. Preliminary results were already presented at the annual DGHM-meeting at Kiel (Winkler, 1967).


Serratia Marcescens RNase Activity Late Logarithmic Phase Phage Stock Nucleolytic Activity 
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  1. Adams, M. H.: Bacteriophages, pp. 592. New York: Interscience Publ. Inc. 1959.Google Scholar
  2. Castañeda-Agullo, M.: J. gen. Physiol. 39, 369 (1956).PubMedCrossRefGoogle Scholar
  3. Catlin, B. W.: Science 124, 441 (1956).PubMedCrossRefGoogle Scholar
  4. Cunningham, L., B. W. Catlin, and M. J. Privat de Garilhe: Amer. Chem. Soc. 78, 4642 (1956).CrossRefGoogle Scholar
  5. Dürwald, H., and H. Hoffmann-Berling: J. mol. Biol. 34, 331 (1968).PubMedCrossRefGoogle Scholar
  6. Eaves, G. N., and C. D. Jeffries: J. Bact. 85, 273 (1963).PubMedGoogle Scholar
  7. Ellmauer, H., and R. W. Kaplan: Naturwissenschaften 46, 150 (1959).CrossRefGoogle Scholar
  8. Frisch-Niggemeyer, W., and K. K. Reddi: Biochem. biophys. Acta (Amst.) 26, 40 (1957).CrossRefGoogle Scholar
  9. Gesteland, R. F.: J. mol. Biol. 16, 67 (1966).PubMedCrossRefGoogle Scholar
  10. Jeffries, C. D., D. F. Holtman, and D. G. Guse: J. Bact. 73, 590 (1957).PubMedGoogle Scholar
  11. Kunitz, M.: J. gen. Physiol. 30, 291 (1946).CrossRefGoogle Scholar
  12. Labrum, E. L. and M. I. Bunting: J. Bact. 65, 394 (1953).PubMedGoogle Scholar
  13. Latarjet, R. P., P. Morenne, and R. Berger: Ann. Inst. Pasteur 85, 174 (1953).Google Scholar
  14. Lederberg, J., and E. Lederberg: J. Bact. 63, 399 (1952).PubMedGoogle Scholar
  15. Leschinskaja, I. B., V. I. Tanjashin, B. M. Kurinenko, and N. Kalacheva: Mikrobiologia 35, 1094 (1966).Google Scholar
  16. Nishimura, S., and M. Nomura: Biochem. biophys. Acta (Amst.) 30, 430 (1958).CrossRefGoogle Scholar
  17. Novick, R. P.: J. gen. Microbiol. 33, 121 (1963).PubMedGoogle Scholar
  18. Radding, C. H.: Proc. nat. Acad. Sci. (Wash.) 52, 965 (1964).CrossRefGoogle Scholar
  19. Radding, C. H.: J. molec. Biol. 18, 251 (1967).CrossRefGoogle Scholar
  20. Richmond, M. H.: Brit. med. Bact. 21, 360 (1965).Google Scholar
  21. Rothberg, N. W., and M. N. Swartz: J. Bact. 90, 294 (1965).PubMedGoogle Scholar
  22. Steiger, H.: Z. allg. Mikrobiol. 4, 367 (1964).CrossRefGoogle Scholar
  23. Takai, N., T. Uchida, and F. Egami: Biochem. biophys. Acta (Amst.) 128, 218 (1966).Google Scholar
  24. Winkler, U.: Z. Naturforsch. 18b, 118 (1963).Google Scholar
  25. Winkler, U.: Virology 24, 518 (1964).PubMedCrossRefGoogle Scholar
  26. Winkler, U.: Zbl. Bact. I. Orig., 205, 62 (1967).Google Scholar
  27. Winkler, U. and K. Heil; Photochem. Photobiol., in press (1968).Google Scholar

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© Springer-Verlag Berlin · Heidelberg 1968

Authors and Affiliations

  • U. Winkler
    • 1
  1. 1.Institut für MikrobiologieJ. W. Goethe-UniversitätFrankfurt/M.Deutschland

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