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Mutants of Serratia Marcescens Defective or Superactive in the Release of a Nuclease

  • U. Winkler

Abstract

During growth several microorganisms, belonging to different systematic groups, release nucleases into the medium. One of these is Serratia marcescens (Jeffries et al., 1957; Rothberg and Swartz, 1965). Bacteria of this species presumably excrete only a single nuclease which degrades both, DNA and RNA, as revealed by biochemical studies (Eaves and Jeffries, 1963). Since extracellular nucleases are expected to be troublesome in certain experiments, e.g. transfections, we were interested in mutants of S. marcescens showing no more extracellular nucleolytic activity. While isolating these socalled nuc - mutants we found accidentally some others (nuc su ) which were superactive in excreting nuclease. This paper describes some of the properties of these nuc - and nuc su mutants. Preliminary results were already presented at the annual DGHM-meeting at Kiel (Winkler, 1967).

Keywords

Serratia Marcescens RNase Activity Late Logarithmic Phase Phage Stock Nucleolytic Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1968

Authors and Affiliations

  • U. Winkler
    • 1
  1. 1.Institut für MikrobiologieJ. W. Goethe-UniversitätFrankfurt/M.Deutschland

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