Mutants of Serratia Marcescens Defective or Superactive in the Release of a Nuclease
During growth several microorganisms, belonging to different systematic groups, release nucleases into the medium. One of these is Serratia marcescens (Jeffries et al., 1957; Rothberg and Swartz, 1965). Bacteria of this species presumably excrete only a single nuclease which degrades both, DNA and RNA, as revealed by biochemical studies (Eaves and Jeffries, 1963). Since extracellular nucleases are expected to be troublesome in certain experiments, e.g. transfections, we were interested in mutants of S. marcescens showing no more extracellular nucleolytic activity. While isolating these socalled nuc - mutants we found accidentally some others (nuc su ) which were superactive in excreting nuclease. This paper describes some of the properties of these nuc - and nuc su mutants. Preliminary results were already presented at the annual DGHM-meeting at Kiel (Winkler, 1967).
KeywordsPermeability Hydrolysis Agar Toluene Sedimentation
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- Adams, M. H.: Bacteriophages, pp. 592. New York: Interscience Publ. Inc. 1959.Google Scholar
- Latarjet, R. P., P. Morenne, and R. Berger: Ann. Inst. Pasteur 85, 174 (1953).Google Scholar
- Leschinskaja, I. B., V. I. Tanjashin, B. M. Kurinenko, and N. Kalacheva: Mikrobiologia 35, 1094 (1966).Google Scholar
- Richmond, M. H.: Brit. med. Bact. 21, 360 (1965).Google Scholar
- Takai, N., T. Uchida, and F. Egami: Biochem. biophys. Acta (Amst.) 128, 218 (1966).Google Scholar
- Winkler, U.: Z. Naturforsch. 18b, 118 (1963).Google Scholar
- Winkler, U.: Zbl. Bact. I. Orig., 205, 62 (1967).Google Scholar
- Winkler, U. and K. Heil; Photochem. Photobiol., in press (1968).Google Scholar