In Vitro Colony Growth of Acute Myelogenous Leukemia

  • K. A. Dicke
  • G. Spitzer
  • P. H. M. Scheffers
  • A. Cork
  • M. J. Ahearn
  • B. Löwenberg
  • K B. McCredie
Conference paper
Part of the Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 19)


Colony formation in vitro by marrow cells from patients with untreated acute myelogenous leukemia (AML) and from patients in AML relapse is infrequent using the standard Robinson assay. A newly developed culture system has been described in which marrow from AML patients in these disease stages form leukemic cell colonies. In this in vitro system, phytohaemagglutinin is the essential stimulator for colony formation. The leukemic origin of the colonies has been proven by ultrastructural morphology and cytogenetics. It appears that colony formation by leukemic cells in this system is predominantly independent from the leukocyte factor which is the main stimulator in the Robinson assay for growing colonies of marrow cells from haematologically normal individuals.


Leukemic Cell Bone Marrow Cell Marrow Cell Colony Formation Acute Leukemia 
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Copyright information

© J. F. Lehmanns Verlag München 1976

Authors and Affiliations

  • K. A. Dicke
    • 1
  • G. Spitzer
    • 1
  • P. H. M. Scheffers
    • 1
  • A. Cork
    • 1
  • M. J. Ahearn
    • 1
  • B. Löwenberg
    • 1
  • K B. McCredie
    • 1
  1. 1.University of Texas System Cancer CenterM. D. Anderson Hospital and Tumor Institute HoustonUSA

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