Immunological Membrane Markers of Hodgkin’s Cells
Reed-Sternberg and other Hodgkin’s giant cells derived from involved spleens and lymph nodes of patients with Hodgkin’s disease were examined for surface markers of T and B cells and macrophages. Attachment and phagocytosis of untreated (E) or sensitized (EA and EAC) sheep red blood cells and yeast by Reed-Sternberg cells did not occur. IgG frequently detected at the membrane of Reed-Sternberg cells was partially removed by incubation and washing at 37 °C. Fluorescence with a specific anti-T cell serum was not seen on Reed-Sternberg and other Hodgkin’s giant cells. These studies indicate that Reed-Sternberg and other Hodgkin’s giant cells lack most detectable normal human lymphoid cell markers, but do exhibit membrane bound immunoglobulin possibly of exogenous origin.
KeywordsChronic Lymphocytic Leukemia Latex Particle Mycosis Fungoides Complement Receptor Sheep Erythrocyte
Fetal calf serum
bovine serum albumin-phosphate buffered saline
chronic lymphocytic leukemia
surface-bound immunoglobulin G
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