Skip to main content
SpringerLink
Log in
Book cover

Laboratory Techniques in Membrane Biophysics pp 157–166Cite as

Dissection of Single Nerve Fibres and Measurement of Membrane Potential Changes of Ranvier Nodes by means of the Double Air Gap Method

  • Chapter

Abstract

A sciatic gastrocnemius nerve-muscle preparation is obtained in the usual way. It is lifted into air by holding the achilles tendon with a forceps. The glass plate for dissection is then approached from the flat side of the muscle and the preparation brought to rest on it without distorting the nerve. The plate is put on a water-cooled dissection stand. A circular neon-bulb situated underneath provides a dark field illumination.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barillot, J.-Cl.: Etude électrophysiologique comparée des fibres nerveuses myélinisées motrices et sensitives chez Rana esculenta et Xenopus laevis. Thèse du 3ème Cycle Poitiers (1966).

    Google Scholar 

  2. Bergman, C., et R. Stämpfli: Différence de perméabilité des fibres nerveuses myélinisées sensorielles et motrices à l’ion potassium. Helv. physiol. Pharmacol. Acta 24, 247–258 (1966).

    CAS  Google Scholar 

  3. Huxley, A. F., and R. Stämpfli: Effect of potassium and sodium on resting and action potentials of single myelinated nerve fibres. J. Physiol. (Lond.) 112, 476–495 (1951).

    CAS  Google Scholar 

  4. Nonner, W.: A new voltage clamp method for ranvier nodes. Pflügers Arch. (In preparation to appear in 1969).

    Google Scholar 

  5. Nonner, W., and R. Stämpfli: A new voltage clamp method, p. 171. (This volume.)

    Google Scholar 

  6. Stämpfli, R.: Bau und Funktion isolierter markhaltiger Nervenfasern. Ergebn. Physiol. 47, 70–165 (1952).

    PubMed  Google Scholar 

  7. Stämpfli, R.: Nouvelle méthode pour enregistrer le potentiel d’action d’un seul étrang-lement de Ranvier et sa modification par un brusque changement de la concentration ionique du milieu extérieur. J. Physiol. (Paris) 48, 710–714 (1956).

    Google Scholar 

Download references

Authors
  1. R. Stämpfli
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. II. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

    H. Passow

  2. I. Physiologisches Institut, Universität des Saarlandes, Homburg a. d. Saar, Germany

    R. Stämpfli

Rights and permissions

Reprints and permissions

Copyright information

© 1969 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Stämpfli, R. (1969). Dissection of Single Nerve Fibres and Measurement of Membrane Potential Changes of Ranvier Nodes by means of the Double Air Gap Method. In: Passow, H., Stämpfli, R. (eds) Laboratory Techniques in Membrane Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87259-4_13

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-87259-4_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-04592-2

  • Online ISBN: 978-3-642-87259-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation