Skip to main content

The Form of Nerve Cells: Determination by Cobalt Impregnation

  • Chapter

Abstract

Form and function are perhaps more closely related in neurons than in any other cell type. A major approach to understanding the cellular events underlying behavior concentrates on relatively simple invertebrate systems that give rise to specific behavioral acts. The general procedure followed is (1) to identify the individual neurons that generate the behavior, and (2) to analyze the particular pattern of connections between neurons to determine the properties of the circuit that might directly govern the form of the behavioral act. Initial studies dealt primarily with identification of nerve cell bodies because of their relative accessibility. This led to the production of neuron soma maps for several invertebrates (Hughes and Tauc, 1962; Otsuka et al., 1967; Nicholls and Baylor, 1968). One such cell body map for the cockroach is shown in Fig. 1 (Cohen and Jacklet, 1967). The distribution of about 50 bilateral pairs of motoneurons is defined in relation to the nerve trunks through which their axons leave the ganglion.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Blackstad, T. W.: Electronmicroscopy of Golgi preparations for the study of neuronal relations. In: Contemporary Research Methods in Neuroanatomy. Ed. W. J. H. Nauta and S. O. E. Ebbesson. pp. 186–216. New York: Springer-Verlag, 1970.

    Google Scholar 

  • Cohen, M. J.: A comparison of invertebrate and vertebrate central neurons. In: The Neurosciences: Second Study Program. Ed. F. O. Schmitt, pp. 798–812. New York: Rockefeller University Press, 1970.

    Google Scholar 

  • Cohen, M. J., and J. W. Jacklet: The functional organization of motor neurons in an insect ganglion. Phil. Trans. R. Soc., Lond. B 252, 561–572 (1967).

    Article  Google Scholar 

  • Hoyle, G.: Cellular mechanisms underlying behavior. In: Advances in Insect Physiology. Ed. J. W. L. Beament, J. E. Treherne and V. B. Wigglesworth. pp. 349–444. London: Academic Press, 1970.

    Google Scholar 

  • Hughes, G. M., and L. Tauc: Aspects of the organization of central nervous pathways in Aplysia depilans. J. exp. Biol. 39, 45–69 (1962).

    PubMed  CAS  Google Scholar 

  • Iles, J. F., and B. Mulloney: Procion yellow staining of cockroach motor neurones without the use of microelectrodes. Brain Res. 30, 397–400 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixation of high osmolality for use in electron microscopy. J. Cell. Biol. 27, 137A (1965).

    Google Scholar 

  • Kerkut, G. A., R. M. Pitman, and R. J. Walker: Iontophoretic application of acetylcholine and GABA onto insect central neurones. Comp. Biochem. Physiol. 31, 611–633 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Nicholls, J. G. and D. A. Baylor: Specific modalities and receptive fields of sensory neurons in CNS of the leech. J. Neurophysiol. 31, 740–756 (1968).

    PubMed  CAS  Google Scholar 

  • Otsuka, M., E. A. Kravitz, and D. D. Potter: Physiological and chemical architecture of a lobster ganglion with particular reference to gamma-aminobutyrate and glutamate. J. Neurophysiol. 30, 725–752 (1967).

    PubMed  CAS  Google Scholar 

  • Pitman, R. M., C. D. Tweedle, and M. J. Cohen: Branching of central neurons: intracellular cobalt injection for light and electron microscopy. Science 176, 412–414 (1972a).

    Article  PubMed  CAS  Google Scholar 

  • Pitman, R. M., C. D. Tweedle, and M. J. Cohen: Electrical responses of insect central neurons: augmentation by nerve section or colchicine. Science 178, 507–509 (1972b).

    Article  PubMed  CAS  Google Scholar 

  • Shepherd, G. M.: The olfactory bulb as a simple cortical system: experimental analysis and functional implications. In: The Neurosciences: Second Study Program. Ed. F. O. Schmitt, pp. 539–552. New York: The Rockefeller University Press, 1970.

    Google Scholar 

  • Spurr, A. R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Stretton, A. O. W., and E. A. Kravitz: Neuronal geometry: determination with a technique of intracellular dye injection. Science 162, 132–134 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Trujillo-Cenoz, O., and J. Melamed: Light and electronmicroscope study of one of the systems of centrifugal fibers found in the lamina of muscoid flies. Z. Zeilforsch. mikrosk. Anat. 110, 336–349 (1970).

    Article  CAS  Google Scholar 

  • Zawarzin, A.: Zur Morphologie der Nervenzentren. Das Bauchmark der Insekten. Ein Beitrag zur vergleichenden Histologie (Histologische Studien über Insekten VI). Z. wiss. Zool. 122, 323–424 (1924).

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1973 Springer-Verlag New York Inc.

About this chapter

Cite this chapter

Pitman, R.M., Tweedle, C.D., Cohen, M.J. (1973). The Form of Nerve Cells: Determination by Cobalt Impregnation. In: Kater, S.B., Nicholson, C. (eds) Intracellular Staining in Neurobiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87123-8_6

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-87123-8_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-87125-2

  • Online ISBN: 978-3-642-87123-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics