The Chemistry of Reactive Dyes and its Relevance to Intracellular Staining Techniques
The Procion dyes used so successfully for intracellular staining were originally developed for the textile industry, and little information on their chemistry has been readily available to neurobiologists. The initial part of this chapter deals with the rationale behind the use of Procion dyes in the textile industry and describes the basic chemistry of the dyes. This provides a framework for extension of their use to intracellular staining. Specific questions relating to intracellular staining are dealt with in the second part of the chapter.
KeywordsCellulose Hydrolysis Steam Uranium Chlorine
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