Determination of Neuronal Membrane Properties Using Intracellular Staining Techniques
As previous chapters in this book demonstrate, recently developed Procion dye injection techniques make it possible to study both the electrophysiological and the morphological properties of single neurons. This chapter describes how the electrophysiological and anatomical information derived from the use of dye-filled pipettes can be used to calculate passive membrane properties of nerve cells. Knowledge of the membrane parameters enables prediction of the size and relative effectiveness of synaptic potentials originating on the dendritic tree (Rall, 1962, 1967, 1970). In order to obtain accurate data, particular attention must be paid to micropipette recording techniques, and these will be discussed in the first part of this chapter. The experiments reported here were performed on cat motoneurons, but the techniques should be applicable to many other cells.
KeywordsDendritic Tree Voltage Response Synaptic Potential Concentric Electrode Input Admittance
Unable to display preview. Download preview PDF.
- Barrett, J. N.: The passive properties of cat motoneurons and their influence on the effective ness of dendritic synapses. Ph.D. thesis. University of Washington, Seattle, Washington, U.S.A. (1972).Google Scholar
- Burke, R. E., and G. ten Bruggencate: Electrotonic characteristics of alpha motoneurones of varying size. J. Physiol., Lond. 212, 1–20 (1971).Google Scholar
- Crawford, G. N. C., and R. Barer: The action of formaldehyde on living cells as studied by phase contrast microscopy. Q. Jl microsc. Sci. 92, 403–452 (1951).Google Scholar
- Lux, H. D., P. Schubert, and G. W. Kreutzberg: Direct matching of morphological and electrophysiological data in cat spinal motoneurons. In: Excitatory Synaptic Mechanisms. Ed. P. Anderson and J. K. S. Jansen. pp. 189–198. Oslo: Universitetsforlaget, 1970b.Google Scholar
- McMahan, U. J., and D. Purves: An electron-microscopic study of a physiologically identified motoneurone in the leech C.N.S. after injection of the fluorescent dye Procion yellow. J. Physiol., Lond. 222, 64–66 (1972).Google Scholar
- Rall, W.: Theory of physiological properties of dendrites. Ann. N.Y. Acad. Sci. 96, 1071–1092.Google Scholar
- Rall, W.: Time constants and electrotonic length of membrane cylinders and neurons. Biophys. J. 9, 1482–1508 (1969).Google Scholar
- Rall, W.: Cable properties of dendrites and effect of synaptic location. In: Excitatory Synaptic Mechanisms, Proceedings of the Fifth International Meeting of Neurobiologists. Ed. P. Andersen and J. K. S. Jansen. Oslo: Universitetsforlaget, 1970.Google Scholar
- Westrum, R. E., and R. D. Lund: Formalin perfusion for correlative light- and electron-microscopical studies of the nervous system. J. Cell Sci. 1, 229–238 (1966).Google Scholar
- Peter, A., S. L. Palay, and H. deF Webster: The fine structure of the nervous system: the cells and their processes. New York: Harper and Row, 1970Google Scholar
- Burke, R. E. and G. ten Bruggencate: Electronic characteristics of alpha motoneurones of varying size. J. Physiol., Lond. 212, 1–20.Google Scholar