The Definition of a Germinal Center Area as Distinct from the Thymus-Dependent Area in the Lymphoid Tissue of the Mouse

  • M. A. B. de Sousa
  • D. M. V. Parrott

Abstract

It has been observed recently that lymphocyte depletion, often cited as a characteristic of neonatally thymectomized animals, was confined to certain specific areas (thymus-dependent areas) in the lymphoid organs of the mouse, namely around the central arteriole within the splenic follicle and the mid and deep cortex of the lymph nodes (Parrott, De Sousa, and East, 1966). By contrast, the rest of the follicle in the spleen and the outer cortical zone of the lymph nodes, where germinal center development normally occurs, remained primarily uninfluenced by the effects of thymectomy.

Keywords

Saccharide Sodium Chloride Polysaccharide Germinal Peri 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Burwell, R. G.: Studies on the primary and the secondary immune responses of lymph nodes draining homografts of fresh cancellous bone (with particular reference to mechanisms of lymph node reactivity). Ann. N.Y. Acad. Sci. 99, 821–860 (1962).PubMedCrossRefGoogle Scholar
  2. Congdon, C. C, and T. Makinodan: Splenic white pulp alteration after antigen injection: relation to time of serum antibody production. Amer. J. Path. 39, 697–710 (1961).PubMedGoogle Scholar
  3. Cottier, H., E. P. Cronkite, C. R. Jansen, K. R. Rax, S. Singer and C. R. Sipe: Studies on lymphocytes. III. Effects of extracorporeal irradiation of the circulating blood upon the lymphoreticular organs in the calf. Blood 24, 241–253 (1964).PubMedGoogle Scholar
  4. East, J., and D. M. V. Parrott: Operative technique for newborn mice using anaesthesia by cooling. J. Endocrin. 24, 249 (1962).CrossRefGoogle Scholar
  5. Gowans, J. L., and E. J. Knight: The route of recirculation of lymphocytes in the rat. Proc. Roy. Soc. (Lond.) ser. B. 159, 257–282 (1964).CrossRefGoogle Scholar
  6. Hanna, M. G.: Germinal center changes and plasma cell reaction during the primary immune response. Int. Arch. Allerg. 26, 230–251 (1965).PubMedCrossRefGoogle Scholar
  7. Hanna, M. G., Jr., C. C Congdon, and C. J. Wust: Effect of antigen dose on lymphatic tissue germinal center changes. Proc. Soc. exp. Biol. (N.Y.) 121, 286–290 (1966).Google Scholar
  8. Humphrey, J. H., D. M. V. Parrott and J. East: Studies on globulin and antibody production in mice thymectomized at birth. Immunology 7, 419–439 (1964).PubMedGoogle Scholar
  9. Langevoort, H. L.: The histopathology of the antibody response. I. Histogenesis of the plasma cell reaction in rabbit spleen. Lab. Invest. 12, 106–118 (1963).PubMedGoogle Scholar
  10. Mcgregor, D. D., and J. L. Gowans: The antibody response of rats depleted of lymphocytes by dironic drainage from the thoracic duct. J. exp. Med. 117, 303–320 (1962).CrossRefGoogle Scholar
  11. Movat, H. Z., and N. V. P. Fernando: The fine structure of the lymphoid tissue during antibody formation. Exper. mol. Path. 4, 155–188 (1965).CrossRefGoogle Scholar
  12. Nossal, G. J. V., G. L. Ada and C. M. Austin: Antigens in Immunity. IV. Cellular localization of 125C- and 131I-labelled flagella in lymph nodes. Aust. J. exp. Biol. Med. Sci. 42, 311–330 (1964).PubMedCrossRefGoogle Scholar
  13. Nossal, G. J. V., G. L. Ada and, C. M. Austin, J. Pye, and J. Mitchell: Antigens in Immunity. XII. Antigen trapping in the spleen. Int. Arch. Allerg. 29, 368–383 (1966).PubMedCrossRefGoogle Scholar
  14. Oort, J., and J. K. Turk: A histological and autoradiographic study of lymph nodes during the development of contact sensitivity in the guinea pig. Brit. J. exp. Path. 46, 147–154 (1965).PubMedGoogle Scholar
  15. Parrott, D. M. V., M. A. B. de Sousa and J. East: Thymus-dependent areas in the lymphoid organs of neonatally thymectomized mice. J. exp. Med. 123, 191–203 (1966).PubMedCrossRefGoogle Scholar
  16. Parrott, D. M. V., M. A. B. De Sousa and J. East: The integrity of the germinal centers: an investigation of the differential localization of labeled cells in lymphoid organs. This Symposium.Google Scholar
  17. Parrott, D. M. V., M. A. B. De Sousa and J. East and M. A. B. De Sousa: Changes in the thymus-dependent areas of lymph nodes after immunological stimulation. Nature (Lond.) 1966 (in press).Google Scholar
  18. Scothorne, R. J., and I. A. Mcgregor: Cellular changes in lymph nodes and spleen following skin homografting in the rabbit. J. Anat. (Lond.) 89, 283–292 (1955).Google Scholar
  19. Turk, J. K., and C. J. Heather: A histological study of lymph nodes during the development of delayed hypersensitivity to soluble antigens. Int. Arch. Allerg. 27, 199–212 (1965).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1967

Authors and Affiliations

  • M. A. B. de Sousa
    • 1
    • 2
  • D. M. V. Parrott
    • 1
  1. 1.Imperial Cancer Research FundLondonEngland
  2. 2.Centro de BiologiaCalouste Gulbenkian FoundationOeirasPortugal

Personalised recommendations