De novo Formation and Rapid Growth of Germinal Centers During Secondary Antibody Responses to Tetanus Toxoid in Mice

  • H. Cottier
  • G. Keiser
  • N. Odartchenko
  • M. Hess
  • R. D. Stoner


In considering the kinetic behavior and possible functions of germinal centers during immune responses, experimental conditions wherein de novoformation and growth of these structures can be followed are of special interest. It has been demonstrated (Cottieret al., 1964; Cottier et al., 1966) that a delimited situation exists during secondary antibody responses to tetanus toxoid in mice under the following conditions:
  1. 1.

    a time interval of several months is used between the first and second injection of antigen;

  2. 2.

    the site of secondary stimulation differs from the site of primary immunization;

  • and

  1. 3.

    the booster injection is given at a site where the draining lymph nodes in non-stimulated animals contain no, or only a few, germinal centers.



Regional Lymph Node Germinal Center Tetanus Toxoid Secondary Response Popliteal Lymph Node 
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Copyright information

© Springer-Verlag Berlin · Heidelberg 1967

Authors and Affiliations

  • H. Cottier
    • 1
  • G. Keiser
    • 2
  • N. Odartchenko
    • 3
  • M. Hess
    • 2
  • R. D. Stoner
    • 2
  1. 1.Institute of PathologyUniversity of BernSwitzerland
  2. 2.Medical Research CenterBrookhaven National LaboratoryUptonUSA
  3. 3.Swiss Institute For Experimental Cancer ResearchLausanneSwitzerland

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