Radioautographic Studies of Reticular and Lymphoid Cells in Germinal Centers of Lymph Nodes

  • N. B. Everett
  • R. W. Caffrey Tyler


Earlier studies from our laboratory have shown that there are two populations of small lymphocytes in the rat with respect to rate and site of formation and circulating life span (Caffrey et al., 1962). One of these has a rapid turnover rate with a circulating life span of about 5 days. The other is produced at a slow and rather constant rate which is roughly proportional to the growth rate of young adult animals. It is the latter population which has a long circulating life span and recirculates from blood to lymph. It is believed that at least some of these may have a life span commensurate with that of the animal since heavily labeled small lymphocytes have been recovered for as long as one year after labeling with Thymidine-3H. Subsequent studies have shown the percentages of the two populations in blood, thoracic duct lymph, and in each of the major lymphoid organs. It was determined that approximately 75% of the small lymphocytes of the mesenteric lymph node were of the long-lived variety and approximately 25% were short-lived (Everett et al., 1964 a). From attempts to assess the respective functions of the two varieties of small lymphocytes, evidence was obtained which suggested that the long-lived lymphocyte has an active role in immune response mechanisms. For example, these cells evidenced enlargement, both nuclear and cytoplasmic, in animals treated with pertussis vaccine (Everett et al., 1964 a). Additionally, as an indirect response to total body irradiation (Everett et al, 1964 b), many long-lived cells enlarged and showed a pronounced increase in the rate of protein synthesis (Everett et al., 1965).


Lymphoid Cell Germinal Center Mesenteric Lymph Node Total Body Irradiation Pertussis Vaccine 
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  1. Caffrey, R. W., W. O. Rieke, and N. B. Everett: Radioautographic studies of small lymphocytes in the thoracic duct of the rat. Acta Haematol (Basel) 28, 145–154 (1962).CrossRefGoogle Scholar
  2. Caffrey, R. W., N. B. Everett and W. O. Rieke: Radioautographic studies of reticular and blast cells in the hemopoietic tissues of the rat. Anat. Ree. (In press).Google Scholar
  3. Cottier, H., N. Odartchenko, G. Keiser, M. Hess, and R. D. Stoner: Incorporation of tritiated nucleosides and amino acids into lymphoid and plasmocytoid cells during secondary response to tetanus toxoid in mice. Ann. N.Y. Acad. Sci. 113, 612–626 (1964).PubMedCrossRefGoogle Scholar
  4. Everett, N. B., R. W. Caffrey, and W. O. Rieke: Recirculation of lymphocytes. Ann. N.Y. Acad. Sci. 113, 887–897 (1964 a).PubMedCrossRefGoogle Scholar
  5. Everett, N. B., R. W. Caffrey, and W. O. Rieke: Radioautographic studies of the effect of irradiation on the long-lived lymphocyte of the rat. Radiation Res. 21, 383–393 (1964 b).PubMedCrossRefGoogle Scholar
  6. Everett, N. B., R. W. Caffrey, and W. O. Rieke, and M. R. Schwarz: Protein synthesis in lymphocytes. In: The use of radioautography in investigating protein synthesis. New York and London: Academic Press 1965, pp. 143–158.Google Scholar
  7. Fliedner, T. M., M. Kesse, E. P. Cronkite, and J. S. Robertson: Cell proliferation in germinal centers of the rat spleen. Ann. N.Y. Acad. Sci. 113, 587–594 (1964).Google Scholar
  8. Ginsburg, H., and L. Sachs: Destruction of mouse and rat embryo cells in tissue culture by lymph node cells from unsensitized rats. J. Cell comp. Physiol. 66, 199–220 (1965).CrossRefGoogle Scholar
  9. Gowans, J. L., and E. J. Knight: The route of re-circulation of lymphocytes in the rat. Proc. Roy. Soc. (Lond.) Ser. B 159, 257–282 (1964).CrossRefGoogle Scholar
  10. Harris, P. F., G. Haigh, and J. H. Kugler: Observations on the accumulation of mononuclear cells and the activities of the reticulum cells in bone marrow of guinea pigs recovering from whole body irradiation. Acta Fiaematol. (Basel) 29, 166–179 (1963).CrossRefGoogle Scholar
  11. Rieke, W. O., R. W. Caffrey, and N.B. Everett: Rates of proliferation and interrelationships of cells in the mesenteric lymph node of the rat. Blood 22, 674–689 (1963).PubMedGoogle Scholar
  12. Swartzendruber, D. C., and M. G. Hanna Jr.: Electron microscopic autoradiography of germinal center cells in the mouse spleen. J. Cell Biol. 25, 109–119 (1965).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1967

Authors and Affiliations

  • N. B. Everett
  • R. W. Caffrey Tyler
    • 1
  1. 1.Department of Biological StructureUniversity of WashingtonSeattleUSA

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