Lymphatic Tissue Germinal Center Localization of 125I-labeled Heterologous and Isologous Macroglobulins
Recent reports using both particulate and soluble antigens have demonstrated lymphatic tissue germinal centers as structures involved in localization of antigen (Ada et al. 1965, Nossal et al. 1964, and White, 1963). White (1963), who used the fluorescent labeled antibody technique to study the localization of human serum albumin during a secondary response in rabbit spleen, reported a persistence of antigen in spleen lymphatic germinal centers. Nossal and associates (1964) demonstrated early localization of antigen in primary follicles of rat popliteal lymph nodes locally injected with 125I-laheled Salmonella flagella. This was followed by the formation of germinal centers with a decreasing amount of label found in the hyperplastic centers several weeks after injection. Thus, it was suggested that as antigen retention in lymphoid follicles precedes the formation of germinal centers in these nodes, there is a causal relationship between these two phenomena. This supposition supports the idea that each germinal center is a response to one particular antigen and this well may be true initially in the formation of the center. However, in spleen and other lymph nodes of conventional young adult animals there are pre-existing germinal centers which have been described to systematically respond to specific antigen (Congdon, 1964; Congdon and Makinodan, 1961; Hanna, 1964, and Hanna et al., 1966) and have been considered important in terms of immunologic “memory” (Hanna et al., 1966; Thorbecke and Hurlimann, 1965; White, 1965); hence, the retention of antigens in these structures is extremely relevant.
KeywordsSucrose Carbide Albumin Cage Titration
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