Lymphatic Tissue Germinal Center Localization of 125I-labeled Heterologous and Isologous Macroglobulins

  • M. G. HannaJr.
  • T. Makinodan
  • W. D. Fisher

Abstract

Recent reports using both particulate and soluble antigens have demonstrated lymphatic tissue germinal centers as structures involved in localization of antigen (Ada et al. 1965, Nossal et al. 1964, and White, 1963). White (1963), who used the fluorescent labeled antibody technique to study the localization of human serum albumin during a secondary response in rabbit spleen, reported a persistence of antigen in spleen lymphatic germinal centers. Nossal and associates (1964) demonstrated early localization of antigen in primary follicles of rat popliteal lymph nodes locally injected with 125I-laheled Salmonella flagella. This was followed by the formation of germinal centers with a decreasing amount of label found in the hyperplastic centers several weeks after injection. Thus, it was suggested that as antigen retention in lymphoid follicles precedes the formation of germinal centers in these nodes, there is a causal relationship between these two phenomena. This supposition supports the idea that each germinal center is a response to one particular antigen and this well may be true initially in the formation of the center. However, in spleen and other lymph nodes of conventional young adult animals there are pre-existing germinal centers which have been described to systematically respond to specific antigen (Congdon, 1964; Congdon and Makinodan, 1961; Hanna, 1964, and Hanna et al., 1966) and have been considered important in terms of immunologic “memory” (Hanna et al., 1966; Thorbecke and Hurlimann, 1965; White, 1965); hence, the retention of antigens in these structures is extremely relevant.

Keywords

Sucrose Carbide Albumin Cage Titration 

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References

  1. Ada, G. L., G. J. V. Nossal, and J. Pye: Antigens in immunity. XL The uptake of antigen in animals previously rendered immunologically tolerant. Austr. J. exp. Biol. med. Sci. 43, 337–344 (1965).CrossRefGoogle Scholar
  2. Anderson, N. G., H. P. Barringer, E. F. Babelay, and W. D. Fisher: The B-IV zonal ultracentrifuge. Life Sci. 3, 667–671 (1964).PubMedCrossRefGoogle Scholar
  3. Cohen, M. W., and G. J. Thorbecke: Specificity of reaction to antigenic stimulation in lymph nodes of immature rabbits. II Suppression of local morphologic reactions to alum precipitated bovine serum albumin by intraperitoneal injections of soluble bovine serum in neonatal rabbits. J. Immunol. 93, 629–636 (1964).Google Scholar
  4. Congdon, C. C.: The early histologic effects of antigen stimulation. Arch. Path. 78, 83–96 (1964).PubMedGoogle Scholar
  5. Congdon, C. C, and T. Makinodan: Splenic white pulp alteration after antigenic injection: relation to time of serum antibody production. Amer. J. Path. 39, 697–709 (1961).PubMedGoogle Scholar
  6. Fisher, W. D., and R. E. Canning: Isolation characterization of rat macroglobulin. J. nat. Cancer Inst. Monograph 22, 1966 (in press).Google Scholar
  7. Garvey, J. S., and D. H. Campbell: The relation of circulating antibody concentration to localization of labeled (S35) antipn. J. Immunol. 72, 131–138 (1954).PubMedGoogle Scholar
  8. Garvey, J. S., and D. H. Campbell: Autoradiographic investigation of tissues after primary and multiple antigenic stimulation of rabbits. Nature (Lond.) 209, 1201–1202 (1966).CrossRefGoogle Scholar
  9. Hanna, M. G., jr.: An autoradiographic study of the germinal center in spleen white pulp during early intervals of the immune response. Lab. Invest. 13, 95–104 (1964).PubMedGoogle Scholar
  10. Hanna, M. G., jr.: Germinal center changes and plasma cell reaction during primary immune response. Intern. Arch. Allergy, appl. Immunol. 26, 230–251 (1965).CrossRefGoogle Scholar
  11. Hanna, M. G., jr. C. C Congdon, and C. J. Wust: Effect of antigen dose on lymphatic tissue germinal centers. Proc. Soc. exp. Biol. (N.Y.) 121, 286–290 (1966)Google Scholar
  12. Hanna, M. G., jr., D. C. Swartzendruber, and C. C Congdon: Morphologic changes in spleen lymphatic tissue during antibody production. Exp. mol. Path. Suppl. 3, 75–87 (1966).Google Scholar
  13. Haurowitz, F., and C. F. Crampton: The fate in rabbits of intravenously injected I131-Iodo albumin. J. Immunol. 68, 73–85 (1952).PubMedGoogle Scholar
  14. Ingraham, J. S.: Artificial radioactive antigens. J. infect. Dis. 89, 109–116 (1951).PubMedCrossRefGoogle Scholar
  15. Layne, E.: In Methods in enzymology III. New York: Academic Press 1957, pp. 448–450.Google Scholar
  16. Mcmaster, P. D., H. Kruse, E. Sturn, and J. L. Edwards: The persistence of bovine-globulin injected as an antigen into rabbits. J. exp. Med. 100, 341–361 (1964).CrossRefGoogle Scholar
  17. Nossal, G. J. v., G. L. Ada, and C. M. Austin: Antigens in immunity. IV. Cellular localization of I131and 131I-labeled flagella in lymph nodes. Austr. J. exp. Biol. Med. Sci. 42, 311–330 (1964).CrossRefGoogle Scholar
  18. Thorbecke, G. J., and J. Hurlimann: Les nodules lymphoides secondaires. Nouv. Rev. Franç Hematol. 5, 383–392 (1965).Google Scholar
  19. White, R. G.: Functional recognition of immunologically competent cells by means of the fluorescent antibody technique. In: The immunologically competent cell. Ciba Foundation study group n0 16. G. E. W. Wolstenholme and J. Knight eds). Boston: Little and Brown 1963, pp. 6–19.Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1967

Authors and Affiliations

  • M. G. HannaJr.
  • T. Makinodan
  • W. D. Fisher
    • 1
  1. 1.Biology Division and NIH-AEC, Co-Carcinogenesis ProjectOak Ridge National LaboratoryOak RidgeUSA

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