Abstract
Rabbits and guinea pigs of the following ages were used for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) reactions: newborn, 3, 6, 9, 12, 16, 20, 25, 30 and 40 days, 3 and 6 months. The animals were anesthetized by ether prior to sacrificing them. The unfixed brains were removed very quickly, cut into 3 or 4 blocks depending upon the size of the brain, and these blocks were frozen with carbon dioxide on freezing stages. On a Dittes-Duspiva cryostat 16 µ thick sections were cut serially. Every fifth section was saved at those levels, where on the basis of previous observations the interstitial neurons in compact fiber bundles were expected to be present. At other levels every 20th section was saved. Most of the sections were used for AChE reaction and some representative sections from each level were used for BChE reaction.
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© 1968 Springer-Verlag Berlin · Heidelberg
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Das, G.D., Kreutzberg, G.W. (1968). Material and Methods. In: Evaluation of Interstitial Nerve Cells in the Central Nervous System. Advances in Anatomy, Embryology and Cell Biology / Ergebnisse der Anatomie und Entwicklungsgechichte / Revues d’anatomie et de morphologie expérimentale, vol 41/1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-86654-8_2
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DOI: https://doi.org/10.1007/978-3-642-86654-8_2
Publisher Name: Springer, Berlin, Heidelberg
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