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Elektronenmikroskopische Untersuchungs- und Präparationsmethoden pp 527–556Cite as

Präparation organischer Teilchen (Fraktionen, Homogenisate, Bakterien, Phagen und Viren)

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Zusammenfassung

Um Bakterienkulturen nach dem Impfen des Nährbodens (Agar) elektronenmikroskopisch zu untersuchen, gibt es folgende Möglichkeiten:

  1. 1.

    Der Nährstoffboden wird direkt mit verdünnter Kollodiumlösung in Amylazetat übergossen (Wyckoff, 1948b). Der getrocknete Kollodiumfilm wird dann mit den haftenden Bakterien auf Wasser abflottiert und auf Trägernetze gefischt. Für dieses und die folgenden Verfahren werden am zweckmäßigsten Glasobjektträger verwendet, die mit einer Agarschicht überzogen werden.

  2. 2.

    Mit Kollodium oder Formvar befilmte Glasplatten werden gegen die Kolonie gepreßt, wobei die Bakterien z. T. hängen bleiben (Abklatschverf ahren). Nach Liebermeister (1953) kann man nach dem Auflegen eines bewachsenen Agarblockes auf eine befilmte Glasplatte auch eine Fixation mit Bouinscher Lösung, die auch sonst in der Agarfixation üblich ist, durchführen. Das Agarstück wird dann vorsichtig abgeschleudert. Die anschließende Wässerung erfolgt durch Schwimmen des Präparates auf Wasser, wobei die Schichtseite nach unten zeigt. Man kann auch gleich den befilmten Agarblock auf die Glasplatte legen und die Kultur zwischen Agarblock und Film wachsen lassen.

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Literatur zu § 23

  • Almeida, J. D., and A. F. Howatson: Substructure of viruses within cells studied by negative staining. V. Internat. Congr. EM Philadelphia, Vol. II, MM-4 (1962).

    Google Scholar 

  • Almeida, J. D., and A. F. Howatson: A negative staining method for cell-associated virus. J. Cell Biol. 16, 616 (1963).

    Article  Google Scholar 

  • Anderson, T. F.: The use of critical point phenomena in prep. specimens for the electr. micr. J. appl. Phys. 21, 724 (1950).

    Article  ADS  Google Scholar 

  • Anderson, T. F.: Stereoscopic studies of cells and viruses in the electr. micr. Amer. Naturalist 86, 91 (1952).

    Article  Google Scholar 

  • Anderson, T. F.: Preservation of structures in dried specimens. Proc. Int. Congr. EM London, 122 (1954).

    Google Scholar 

  • Anderson, T. F.: Electr. micr. of microorganism. In: Phys. Techn. in Biol. Research III (ed. G. Ostern U. A. W. Pollister). New York 1956.

    Google Scholar 

  • Anderson, T. F.: Negative staining and its use in the study of viruses and their serological reactions. In: The Interpretation of Ultrastructure, ed. R. J. C. Harris, 251, New York 1962.

    Google Scholar 

  • Backus, R. C.: Spraying of particulate suspensions containing infective materials for electr. micr. analysis. Science 115, 246 (1952).

    Article  ADS  Google Scholar 

  • Backus, R. C.: Adhesion partitioning, intrasomatic obs. on normal E. Coli and T2 bacteriophage. J. biophys. biochem. Cytol. 1, 99 (1955).

    Article  Google Scholar 

  • Backus, R. C., and R. C. Williams: The use of spraying methods and of volatile suspending media in the prep. of specimens for electr. micr. J. appl. Phys. 21, 11 (1950).

    Article  ADS  Google Scholar 

  • Baker, R. F., and H. E. Pearson: New embedding method for cell suspensions. J. biophys. biochem. Cytol. 9, 217 (1961).

    Article  Google Scholar 

  • Barrera Oro, J. G., K. O. Smith, and J. L. Melnick: Quantitation of Papova virus particles in human warts. V. Internat. Congr. Em Philadelphia. Vol. II, X-3 (1962).

    Google Scholar 

  • Becher, H., u. K. Hoegen: Die Verw. der Hyaluronidase für licht- und elektr. mikr. Präp.methoden biol. Objekte. Z. wiss. Mikr. 62, 41 (1954).

    Google Scholar 

  • Bengtsson, S., and L. Philipson: Countercurrent distribution of poliovirus type 1. Virology 20, 176 (1963).

    Article  Google Scholar 

  • Birbeck, M. S. C., and E. Reid: A new medium for the isolation of liver mitochondria. Biochim. Biophys. Acta 20, 419 (1956).

    Article  Google Scholar 

  • Brandes, J.: Identifizierung von gestreckten pflanzenpathogenen Viren auf morphologischer Grundlage. Mitt. Biol. Bundesanstalt f. Land- u. Forstwirtsch. Berlin-Dahlem 110, 1 (1964).

    Google Scholar 

  • Braun, H., u. H. Ernst: Elektronenopt. Studien zur Isolierung von Kern-Fraktionen. Z. Naturforsch. 15b, 592 (1960).

    Google Scholar 

  • Brenner, S., and R. W. Horne: A negative staining method for high resolution electr. micr. of viruses. Biochim. Biophys. Acta 34, 103 (1959).

    Article  Google Scholar 

  • Bruggen, E. F. J. Van, E. H. Wiebenga, and M. Gruber: Negative-staining electr. micr. of proteins at pH values below their isoelectric points. Its appl. to hemocyanin. Biochim. Biophys. Acta 42, 171 (1960).

    Article  Google Scholar 

  • Bruggen, E. F. J. Van, E. H. Wiebenga, and M. Gruber: Structure and properties of hemocyanins. J. mol. Biol. 4, 1 (1962).

    Article  Google Scholar 

  • Charney, J., R. Machlowitz, A. A. Tytell, J. F. Sagin, and D. S. Spicer: The concentration and purification of poliomyelitis virus by the use of nucleic acid precipitation. Virology 15, 269 (1961).

    Article  Google Scholar 

  • Chauveau, J., Y. Moulé, and C. Rouiller: Isolation of pure and unaltered liver nuclei morphology and biochem. composition. Exp. Cell Res. 11, 317 (1956).

    Article  Google Scholar 

  • Cox, H. R., J. Van Der Scheer, S. Aiston, and E. Bohnel: J. Immunology 56, 149 (1947).

    Google Scholar 

  • Crane, H. R.: Direct centrifugation onto electr. micr. specimen films. Rev. sci. Instr. 15, 253 (1944).

    Article  ADS  Google Scholar 

  • Cuckow, F. W.: Fixation in electr. micr. Nature (Lond.) 175, 131 (1955).

    Article  ADS  Google Scholar 

  • Davison, P. F., and E. H. Mercer: Electr. micr. of cell nuclei isolated in aqueous media. Exp. Cell Res. 11, 237 (1956).

    Article  Google Scholar 

  • Dawson, I. M., and H. Stern: Structure in the bacterial cell wall during cell division. Biochim. Biophys. Acta 13, 31 (1954).

    Article  Google Scholar 

  • Des Jardins, P. R., C. A. Senseney, and G. E. Hess: Further studies in the electr.-micr. of purified tobacco ringspot virus. Phytopath. 43, 687 (1953) [s. a. Science 120, 456 (1954)].

    Google Scholar 

  • Ernster, L., P. Siekevitz, and G. E. Palade: Enzyme-structure relationships in the endoplasmatic reticulum of rat liver. J. Cell Biol. 15, 541 (1962).

    Article  Google Scholar 

  • Fernández-Morkn, H., T. Oda, P. V. Blair, and D. E. Green: A macromolecular repeating unit of mitochondrial structure and function. J. Cell Biol. 22, 63 (1964).

    Article  Google Scholar 

  • Fraser, D.: Bursting bacteria by release of gas pressure. Nature (Lond.) 167, 33 (1951).

    Article  ADS  Google Scholar 

  • Frey-Wyssling, A.: Interpretation of the ultratexture in growing plant cell walls. In: The Interpretation of Ultrastructure I. ed. R. J. C. Harris, 307. New York 1962.

    Google Scholar 

  • Geister, R., u. D. Peters: Ein vereinf. direktes Zählverf. für Virus-Suspensionen ab 105 Partikel/ml. Z. Naturforsch. 18b, 266 (1963).

    Google Scholar 

  • Giesbrecht, P.: Vergl. Unters. an den Chromosomen der Dinollagellaten Amphidinium elegans und denen der Bakterien. Zbl. Bakt. I. Orig. 187, 452 (1962).

    Google Scholar 

  • Grund, S.: “Supercoiling” -System des Chromosoms gramnegativer Bakt. Naturwissenschaften 52, 116 (1965).

    Article  Google Scholar 

  • Guintini, J., and P. T. Tehan: Proc. Conf. EM Delft 1950, S. 159.

    Google Scholar 

  • Hall, C. E.: Electr. micr. of crystalline edestin. J. Biol. Chem. 185, 45. Electr. micr. of cryst. catalase 185, 749 (1950).

    Google Scholar 

  • Hall, C. E.: Electr. densitometry of stained virus particles. J. biophys. biochem. Cytol. 1, 1 (1955).

    Article  Google Scholar 

  • Hall, C. E.: Method for the observation of macromol. with the electr. micr. J. biophys. biochem. Cytol. 2, 625 (1956).

    Article  Google Scholar 

  • Hall, C. E., and P. Doty: A comparison between the dimensions of some macromolecules determined by electr. micr. and by phys. chem. methods. J. Amer. chem. Soc. 80, 1269 (1958).

    Article  Google Scholar 

  • Hartman, R. E., T. D. Green, J. B. Bateman, C. A. Senseney, and G. E. Hess: Prep. of uniformly dispersed spec. of particulate matter for electr. micr. J. appl. Phys. 24, 90 (1953).

    Article  ADS  Google Scholar 

  • Hillier, J., G. Knaysi, and R. F. Baker: New prep. techn. for the electr. micr. of bact. J. Bact. 56, 569 (1948).

    Google Scholar 

  • Hook, A. E., D. Beard, A. R. Taylor, D. G. Sharp, and J. W. Beard: Isolation and characterization of the T2 phage of E. Coli. J. biol. Chem. 165, 241 (1946).

    Google Scholar 

  • Horne, R. W.: The structure and symmetry of virus particles. V. Internat. Congr. EM Philadelphia, Vol. II, S-1 (1962).

    Google Scholar 

  • Horne, R. W., and S. Brenner: A negative staining techn. for high resolution of virus. IV. Internat. Kongr. Em Berlin, Bd. II, 625 (1958).

    Google Scholar 

  • Horne, R. W., and J. Nagington: Electr. micr. studies of the development and structure of poliomyelitis virus. J. mol. Biol. 1, 333 (1959).

    Article  Google Scholar 

  • Horne, R. W., and P. Wildy: Virus structure revealed by negative staining. Adv. Virus Res. 10, 101 (1964).

    Article  Google Scholar 

  • Huxley, H. E.: Some obs. on the structure of TMV. Proc. Stockholm Conf. EM, 260 (1956).

    Google Scholar 

  • Huxley, H. E., and G. Zubay: Electr. micr. obs. on the structure of microsomal particles from E. Coli. J. mol. Biol. 2, 10 (1960).

    Google Scholar 

  • Kaighn, M. E., M. A. Moscarello, and C. R. Fuerst: Purification of mucine encephalomyocarditis virus. Virology 23, 183 (1964).

    Article  Google Scholar 

  • Kanngiesser, W., u. B. Deubner Elektr. mikr. Aufn. von Kartoffel-X-Virus in gefriergetrocknetem Tabakrohsaft. Naturwissenschaften 40, 442 (1953).

    Article  ADS  Google Scholar 

  • Kellenberger, E.: La techn. de prép. microcentrifugation. Experientia (Basel) 5, 253 (1949).

    Article  Google Scholar 

  • Kellenberger, E., and W. Arber Electr. micr. studies of phage multiplication I: A method for the quant. analysis of particle suspensions. Virology 3, 245 (1957).

    Article  Google Scholar 

  • Kellenberger, E., et A. Ryter: Contribution a l’ etude du noyan bacterien. Schweiz. Z. allgem. Path. Bakt. 18, 1122 (1955).

    Google Scholar 

  • Kellenberger, E., et A. Ryter, and J. Séchaud: Electr. micr. study of Dna-containing plasms. J. biophys. biochem. Cytol. 4, 671 (1958).

    Article  Google Scholar 

  • Kleinschmidt, A.: Über die quantitative Spreitung von Zellen. Proc. Stockholm Conf. Em, 128 (1956).

    Google Scholar 

  • Kleinschmidt, A., Lang, C. Plescher, W. Hellmann, J. Haass, R. K. Zahn u. A. Hagedorn: Über die intrazelluläre Formation von Bakterien-DNS. Z. Naturforsch. 16b, 730 (1961).

    Google Scholar 

  • Kleinschmidt, A., u. R. K. Zahn: Über DNS-Molekeln in Protein-Mischfilmen. Z. Naturforsch. 14b, 770 (1959).

    Google Scholar 

  • Klug, A., and J. E. Berger: An opt. method for the analysis of periodicities in electr. micrographs and some obs. on the mechanism of negative staining. J. mol. Biol. 10, 565 (1964).

    Article  Google Scholar 

  • Knaysi, G., J. Hillier, and C. Fabricant: The cytology of an avian stain of mycobacterium tuberculosis studied with the electr. and light micr. J. Bact. 60, 423 (1950).

    Google Scholar 

  • Kuff, E. L., G. H. Hogeboom, and A. J. Dalton: Centrifugal, biochem. and electr. micr. analysis of cytoplasmatic particulates in liver homogenates. J. biophys. biochem. Cytol. 2, 33 (1956).

    Article  Google Scholar 

  • Kühn, K., W. Grassmann, u. U. Hofmann: Die clektr. mikr. “Anfärbung” des Kollagens und die Ausbildung einer hochunterteilten Querstreifung. Z. Naturforsch. 13b, 154 (1958).

    Google Scholar 

  • Kuhnke, E.: Elektr. mikr. Befunde zur Wirkung von Hyaluronidase auf Kollagenfibrillen. Naturwissenschaften 45, 166 (1958).

    Article  ADS  Google Scholar 

  • Laurell, A. H. F.: A method of sectioning bacteria in situ for electr. micr. and cytochem. investigations. Nature (Lond.) 163, 282 (1949).

    Article  ADS  Google Scholar 

  • Lerner, A. M.: Concentration and purification of viruses with special reference to reoviruses. Bact. Rev. 28, 391 (1964).

    Google Scholar 

  • Levinthal, C., and H. Fisher: The struct. development of a bact. virus. Biochim. Biophys. Acta 9, 419 (1952).

    Article  Google Scholar 

  • Liebermeister, K.: Ein Verf. zur Verwendung der Agarfixation in der Elektr. mikr. Z. Naturforsch. 8b, 755 (1953).

    Google Scholar 

  • Lippert, W.: Bemerkungen zum Kontrast beim “negative-staining” der Elektr. mikr. Naturwissenschaften 51, 408 (1964).

    Article  ADS  Google Scholar 

  • Malamed, S.: Use of microcentrifuge for prep. of isolated mitochondria and cell suspensions for electr. micr. J. Cell Biol. 18, 696 (1963).

    Article  Google Scholar 

  • Markham, R., S. Frey, and G. J. Hills: Methods for the enhancement of image detail and accentuation of structure in electr. micr. Virology 20, 88 (1963).

    Article  Google Scholar 

  • Martin, A., and S. G. Tomlin: A techn. for the cultivation and prep. of tissue cultures for electr. micr. Biochim. Biophys. Acta 5, 154 (1950).

    Article  Google Scholar 

  • Mattern, C. F. T., and H. G. Dubuy: Purification and crystallization of coxsackie virus. Science 123, 1037 (1956).

    Article  ADS  Google Scholar 

  • Meyerhoff, K. H., and G. Müller: Anomalous contrast in electr. micr. of negatively stained specimens. Symp. Quant. Electr. micr. Washington 1964, in Lab. Invest. 14, 1080 (1965).

    Google Scholar 

  • Mitchell, R. F.: The examination of some plasma proteins by electr. micr. Biochim. Biophys. Acta 9, 430 (1952).

    Article  Google Scholar 

  • Moll, G.: Elektr. mikr. Unters. zur Virusfiltration mit einem löslichen Al-Alginat-Ultrafilter. Naturwissenschaften 49, 406 (1962).

    Article  ADS  Google Scholar 

  • Moll, G.: Über die lösl. Ultrafilter aus Al- und Lanthan-Alginat und die elektr. mikr. Demonstr. ihrer Filtrationseigenschaften. Z. Hygiene 149, 297 (1963).

    Article  Google Scholar 

  • Moll, G.: Die elektr. mikr. Darst. des Injektionsvorganges von T2-Bakteriophagen und von Phagen-DNS unter Zuhilfenahme von Al-Alginat-Ultrafilter. Naturwissenschaften 52, 21 (1965).

    Article  Google Scholar 

  • Morgan, C., C. Howe, H. M. Rose, and D. H. Moore: Structure and development of viruses obs. in the electr. micr. J. biophys. biochem. Cytol. 2, 351 (1956).

    Article  Google Scholar 

  • Mühlethaler, K.: Gegenwärtiger Stand der elektr. mikr. Erforschung der Pflanzenzelle. Naturwissenschaften 44, 204 (1957).

    Article  ADS  Google Scholar 

  • Müller, G., and K. Meyerhoff: Anomalous contrast in electr. micrographs of negative stained specimens. Nature (Lond.) 201, 590 (1964).

    Article  Google Scholar 

  • Nagington, J., A. A. Newton, and R. W. Horne: The structure of orf virus. Virology 23, 461 (1964).

    Article  Google Scholar 

  • Nilsson, O.: Eine Meth. zur Elektr. mikr. von Zellen aus Gewebekulturen. Z. wiss. Mikr. 62, 441 (1955).

    Google Scholar 

  • Noda, H., and R. W. G. Wyckoff: The electr. micr. of developing bacteriophage. Biochim. Biophys. Acta 8, 381 (1952).

    Article  Google Scholar 

  • Parsons, D. F.: Negative staining of Gross leukemia virus in thinly spread cells and after partial purification. V. Internat. Congr. EM Philadelphia, Vol. II, X-1 (1962).

    Google Scholar 

  • Parsons, D. F.: Negative staining of thinly spread cells and associated virus. J. Cell. Biol. 16, 620 (1963).

    Article  Google Scholar 

  • Petermann, M. L.: The physical and chemical properties of ribosomes. Amsterdam 1964.

    Google Scholar 

  • Peters, D.: Strukturaufkl. am Elementarkörper des Vaccine-Virus durch Abbau mit Trypsin. Proc. Europ. Reg. Conf. EM Delft, Vol. II, 694 (1960).

    Google Scholar 

  • Peters, D., and G. Müller: The fine structure of the DNA-containing core of vaccinia virus. Virology 21, 266 (1963).

    Article  Google Scholar 

  • Peters, D., u. T. Nasemann: Unters. am Virus der Variola-Vaccine. Z. Naturforsch. 8b, 547 (1953).

    Google Scholar 

  • Peters, D., G. Nielssen, u. M. E. Bayer: Variola, die Zuverl. der elektr. mikr. Schnell-diagnostik. Dtsch. Med. Wschr. 87, 2240 (1962).

    Article  Google Scholar 

  • Peters, D., u. R. Wigand: Enzymatisch-elektronenopt. Analyse der Nucleinsäurevert., dargest. an E. Coli als Modell. Z. Naturforsch. 8b, 180 (1953).

    Google Scholar 

  • Pinteric, L., and J. Taylor: The lowered drop method for the prep. of specimens of partially purified virus lysates for quant. electr. micr. Virology 18, 359 (1962).

    Article  Google Scholar 

  • Pohlman, R., u. C. Wolpers: Über das Verh. histologischer Suspensionen im Ultraschallfeld. Kolloid-Z. 109, 106 (1944).

    Article  Google Scholar 

  • Porter, K. R., A. Claude, and E. F. Fullam: A study of tissue culture cells by electr. micr. J. exp. Med. 81, 233 (1945).

    Article  Google Scholar 

  • Raettig, H.: Erf. bei der elektr. opt. Darst. von Bakteriophagen mit dem Filmbewuchsverf. Z. wiss. Mikr. 61, 280 (1953).

    Google Scholar 

  • Reimer, L.: Aufladung kleiner Teilchen im Elektr. mikr. Z. Naturforsch. 20a, 151 (1965).

    ADS  Google Scholar 

  • Rhim, J. S., K. O. Smith, and J. L. Melnick: Complete and coreless forms of reovirus. Ratio of number of virus particles to infective units in the one-step growth cycle. Virology 15, 428 (1961).

    Article  Google Scholar 

  • Rice, R. V., P. Kaesberg, and M. A. Stahmann: The breaking of TMV using a new freeze drying method. Biochim. Biophys. Acta 11, 337 (1953).

    Article  Google Scholar 

  • Rice, R. V., P. Kaesberg, and M. A. Stahmann: Further studies concerning the breaking of TMV. Biochim. Biophys. Acta 20, 488 (1956).

    Article  Google Scholar 

  • Robertis, E. De, C. M. Franchi, and M. Podolsky: Aerosol techn. for the study of macromolecules with the electr. micr. Biochim. Biophys. Acta 11, 507 (1953).

    Article  Google Scholar 

  • Ryter, A., E. Kellenberger, A. Birch-Andersen, and O. Maaloe: Etude au micr. electr. de plasmas contenant de DNA. Z. Naturforsch. 13b, 597 (1958).

    Google Scholar 

  • Salton, M. R. J., and R. W. Horne: Studies of the bacteria cell walls I u. II. Biochim. Biophys. Acta 7, 19, 177 (1951).

    Article  Google Scholar 

  • Schreil, W. H.: Studies on the fixation of artificial and bacterial DNA plasms for the electr. micr. of thin sections. J. Cell Biol. 22, 1 (1964).

    Article  Google Scholar 

  • Sharp, D. G.: Enumeration of virus particles by electr. micr. Proc. Soc. exp. Biol. Med. 70, 54 (1949).

    Google Scholar 

  • Sharp, D. G.: Sedimentation counting of particles via electr. micr. IV. Internat. Kongr. EM Berlin, Bd. II, 542 (1958).

    Google Scholar 

  • Sharp, D. G.: Extraction and counting of vaccinia virus particles from calf lymph vaccine. J. Immunology 84, 507 (1960).

    Google Scholar 

  • Sharp, D. G.: Quant. use of the electr. micr. in virus research. Symp. Quant. Electr. micr. Washington 1964, in Lab. Invest. 14, 831 (1965).

    Google Scholar 

  • Shimuzu, T.: Electr. micr. of tissue cells. A. Schole Med. Univ. Kioto 28, 77 (1950).

    Google Scholar 

  • Smith, K. O., and J. L. Melnick: Recognition and quantitation of herpes-virus particles in human vesicular lesions. Science 137, 543 (1962).

    Article  ADS  Google Scholar 

  • Steere, R. L.: Electr. micr. of structural detail in frozen biol. specimens. J. biophys. biochem. Cytol. 3, 45 (1957).

    Article  Google Scholar 

  • Sugar, I.: A micro-manipulation method for the prep. of calibrated microdroplets over the range of 10–6-10–10 ml in electr. micr. Proc. Stockholm Conf. EM, 127 (1956).

    Google Scholar 

  • Thornley, M. J., and R. W. Horne: Electr. micr. obs. on the structure of fimbriae, with particular ref. to Klebsiella Strains by the use of negative staining techn. J. gen. Microbiol. 28, 51 (1962).

    Article  Google Scholar 

  • Valentine, R. C., and J. R. G. Bradfield: A new procedure for bacterial viability counts and its biophys. appl. Nature (Lond.) 171, 878 (1953).

    Article  ADS  Google Scholar 

  • Valentine, R. C., and R. W. Horne: An assessment of negative staining techn. for revealing ultrastructure. In: Interpretation of Ultrastructure, ed. R. J. C. Harris, 263, New York 1962.

    Google Scholar 

  • Watson, D. H.: Electr. micr. particle counts of phosphotungstate-sprayed virus. Biochim. Biophys. Acta 61, 321 (1962).

    Google Scholar 

  • Weibull, C.: The isolation of protoplasts from bac. megaterium by controlled treatment with lysozyme. J. Bact. 66, 688 (1953).

    Google Scholar 

  • Weibull, C., and J. Hedvall: Some obs. of fractions of disintegrated bacterial cells obtained by diff. centrifugations. Biochim. Biophys. Acta 10, 35 (1953).

    Article  Google Scholar 

  • Weil, M. L., J. Warren, S. S. Breese, S. B. Russ, and H. Jeffries: Separation of encephalomyocarditis virus from tissue components by means of protamine precipitation and encymic digestion. J. Bact. 63, 99 (1952).

    Google Scholar 

  • Williams, R. C.: Method of freeze-drying for electr. micr. Exp. Cell Res. 4, 188 (1953).

    Article  Google Scholar 

  • Williams, R. C., and R. C. Backus: Macromolecular weights determined by direct particle counting I. J. Amer. Chem. Soc. 71, 4052 (1949).

    Article  Google Scholar 

  • Williams, R. C., R. C. Backus, and R. L. Steere Macromol. weights determined by direct particle counting II. J. Amer. Chem. Soc. 73, 2062 (1951).

    Article  Google Scholar 

  • Wyckoff, R. W. G.: Frozen-dried prep. for electr. micr. Science 104, 37 (1946).

    Article  ADS  Google Scholar 

  • Wyckoff, R. W. G.: The electr. micr. of macromol. crystals. Acta cryst. 1, 292 (1948 a).

    Article  Google Scholar 

  • Wyckoff, R. W. G.: The clectr.micr. of developing bacteriophage. Biochim. Biophys. Acta 10, 35 (1948b).

    Google Scholar 

  • Yamamoto, T., and S. Nagasaki: An appl. of analytical study to electr. micr. of yeast cells. Proc. Intern. Congr. EM London, 440 (1954).

    Google Scholar 

  • Zapf, K., u. J. Ludvik: Einf. in die elektr. mikr. Präpariertechn. in der Mikrobiol. Jena 1961.

    Google Scholar 

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    Dr. Ludwig Reimer (apl. Professor)

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Reimer, L. (1967). Präparation organischer Teilchen (Fraktionen, Homogenisate, Bakterien, Phagen und Viren). In: Elektronenmikroskopische Untersuchungs- und Präparationsmethoden. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-86557-2_23

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