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Kontrastierung und histochemische Methoden

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Elektronenmikroskopische Untersuchungs- und Präparationsmethoden

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Zusammenfassung

Die OsO4- und Kaliumpermanganat-Fixation bewirken außer einer Fixation auch gleichzeitig eine Kontrastierung durch Ablagerung von Schwermetallen. Dies demonstrieren vor allem die Fixationsmethoden ohne Metalleinbau — Gefriertrocknung oder Aldehydfixation. Aber auch bei der Aldehydfixation wird Aldehyd bei der Vernetzung miteingebaut und die Dichte der Substanz verändert. Bei einer OsO4-Nachbehandlung von aldehydfixiertem Gewebe wirkt diese vorwiegend als Kontrastierungsmittel, obwohl dabei auch noch einige Fixationsprozesse ablaufen. Eine klare Trennung zwischen Fixation und Kontrastierung läßt sich daher nicht ziehen. Jede Kontrastierung beruht auf chemischen Anlagerungsprozessen, die zu einer weiteren Denaturierung führen.

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© 1967 Springer-Verlag Berlin Heidelberg

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Reimer, L. (1967). Kontrastierung und histochemische Methoden. In: Elektronenmikroskopische Untersuchungs- und Präparationsmethoden. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-86557-2_20

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  • DOI: https://doi.org/10.1007/978-3-642-86557-2_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-86558-9

  • Online ISBN: 978-3-642-86557-2

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