Mitogenic Activity of Polynucleotides on Thymus-Influenced Lymphocytes
An adjuvant effect on antibody synthesis of isologous or heterologous nucleic acids has been known for some time (Jaroslow and Taliaferro, 1956; Merritt and Johnson, 1965). With the recent rapid acquisition of more precise knowledge as to how the immune system functions, the groundwork has been laid to define the cell types and the intracellular biochemical events affected by this adjuvant. In initiating investigation on this aspect, we have studied (Johnson et al., 1971) the characteristics of a nontoxic (Han et al., 1973), double-stranded homoribopolymer, polyadenylic acid complexed to polyuridylic acid (poly A:U). Similar efforts have emerged from Dr. Werner Braun’s laboratory (Braun et al., 1971), with a concentration on experiments linking the poly A:U effect to activation of cyclic AMP in spleen cells (Ishizuka et al., 1971). In our laboratory, we have postponed measurements of cyclic AMP, electing first to define the precise cell type affected by this adjuvant.
KeywordsSpleen Cell Antibody Formation Mitogenic Activity Mitogenic Effect Tritiated Thymidine
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- Han, I. H., Johnson, A. G., Cook, J., and Han, S. S. “Comparative Biological Activity of Endotoxin and Synthetic Polyribonucleotides,” J. Infec. Dis. (in press).Google Scholar
- Marchalonis, J. J., Cone, R. E., and Rolley, R. T. “Amplification of Thymus-Influenced Lymphocytes By Poly A:U: Inhibition of Antigen Binding By Antiserum to Immunoglobulin Light Chain,” J. Immunol. 110:556–561 (1973).Google Scholar
- McNeill, T. A. “The Effect of Synthetic Double-Stranded Polyribonucleotides on Haemopoietic Colony Forming Cells in Vitro,” Immunol. 21:741–750 (1971).Google Scholar
- Mozes, E., Shearer, G. M., Sela, M., and Braun, W. “Conversion with Polynucleotides of a Genetically Controlled Low Immune Response to a High Response in Mice Immunized with a Synthetic Polypeptide Antigen.” Biological Effects of Polynucleotides, R. F. Beers and W. Braun, eds. New York: Springer-Verlag, pp. 197-214 (1971).Google Scholar