Abstract
An adjuvant effect on antibody synthesis of isologous or heterologous nucleic acids has been known for some time (Jaroslow and Taliaferro, 1956; Merritt and Johnson, 1965). With the recent rapid acquisition of more precise knowledge as to how the immune system functions, the groundwork has been laid to define the cell types and the intracellular biochemical events affected by this adjuvant. In initiating investigation on this aspect, we have studied (Johnson et al., 1971) the characteristics of a nontoxic (Han et al., 1973), double-stranded homoribopolymer, polyadenylic acid complexed to polyuridylic acid (poly A:U). Similar efforts have emerged from Dr. Werner Braun’s laboratory (Braun et al., 1971), with a concentration on experiments linking the poly A:U effect to activation of cyclic AMP in spleen cells (Ishizuka et al., 1971). In our laboratory, we have postponed measurements of cyclic AMP, electing first to define the precise cell type affected by this adjuvant.
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References
Braun, W., Ishizuka, M., Yajima, Y., Webb, D., and Winchurch, R. “Spectrum and Mode of Action of Poly A:U in the Stimulation of Immune Responses.” Biological Effects of Polynucleotides, R. F. Beers and W. Braun, eds. New York: Springer-Verlag, pp. 139–156 (1971).
Campbell, P. A., and Kind, P. “Bone Marrow Derived Cells As Target Cells for Polynucleotide Adjuvants,” J. Immunol. 107:1419–1423 (1971).
Cohen, J. J., and Claman, H. N. “Thymus-Marrow Immunocompetence. V. Hydrocortisone-Resistant Cells and Processes in the Hemolytic antibody response of Mice,” J. Exp. Med. 133:1026–1034 (1971).
Cone, R. E., and Johnson, A. G. “Regulation of the Immune System by Synthetic Polynucleotides. III. Action on Antigen-Reactive cells of Thymic Orgin,” J. Exp. Med. 133:665–676 (1971).
Cone, R. E., and Johnson, A. G. “Regulation of the Immune System by Synthetic Polynucleotides. IV. Amplification of Proliferation of Thymus-Influenced Lymphocytes,” Cell. Immunol. 3:283–293 (1972).
Friedman, H. G., Johnson, A. G., and Pan, P. “Stimulatory Effect of Polynucleotides on Short-Term Leucocyte Cultures,” Proc. Soc. Exp. Biol. Med. 132:916–918 (1969).
Han, I. H., Johnson, A. G., Cook, J., and Han, S. S. “Comparative Biological Activity of Endotoxin and Synthetic Polyribonucleotides,” J. Infec. Dis. (in press).
Ishizuka, M., Braun, W., and Matsumoto, T. “Cyclic AMP and Immune Responses. I. Influence of Poly A:U and cAMP on Antibody Formation in Vitro”, J. Immunol. 107:1027–1035 (1971).
Jaroslow, B. N., and Taliaferro, W. H. “Restoration of Hemolysin-Forming Capacity in X-Irradiated Rabbits by Tissue and Yeast Preparations,” J. Infec. Dis. 98:75–81 (1956).
Jaroslow, B. N., and Ortiz-Ortiz, L. “Influence of Poly A-Poly U on Early Events in the Immune Response in Vitro,” Cell. Immunol. 3:123–132 (1972).
Johnson, A. G. “Stimulation of the Immune System by Homopolyribonucleotides.” Biological Effects of Polynucleotides, R. F. Beers and W. Braun, eds. New York: Springer-Verlag, pp. 157–177 (1971).
Johnson, H. G., and Johnson, A. G. “Regulation of the Immune System by Synthetic Polynucleotides. II. Action on Peritoneal Exudate Cells,” J. Exp. Med. 133:649–664 (1971).
Marchalonis, J. J., Cone, R. E., and Rolley, R. T. “Amplification of Thymus-Influenced Lymphocytes By Poly A:U: Inhibition of Antigen Binding By Antiserum to Immunoglobulin Light Chain,” J. Immunol. 110:556–561 (1973).
McNeill, T. A. “The Effect of Synthetic Double-Stranded Polyribonucleotides on Haemopoietic Colony Forming Cells in Vitro,” Immunol. 21:741–750 (1971).
Merritt, K., and Johnson, A. G. “Studies on the Adjuvant Action of Bacterial Endotoxins on Antibody Formation. VI. Enhancement of Antibody Formation by Nucleic Acids,” J. Immunol. 94:416–422 (1965).
Mozes, E., Shearer, G. M., Sela, M., and Braun, W. “Conversion with Polynucleotides of a Genetically Controlled Low Immune Response to a High Response in Mice Immunized with a Synthetic Polypeptide Antigen.” Biological Effects of Polynucleotides, R. F. Beers and W. Braun, eds. New York: Springer-Verlag, pp. 197-214 (1971).
Schmidtke, J. R., and Johnson, A. G. “Regulation of the Immune System by Synthetic Polynucleotides. I. Characteristics of Adjuvant Action on Antibody Synthesis,” J. Immunol. 106:1191–1200 (1971).
Stout, R. D., and Johnson, A. G. “Regulation of the Immune System By Synthetic Polynucleotides. V. Effect on Cell-Associated Immunoglobulin Receptors and Immunological Memory,” J. Exp. Med. 135:45–67 (1972).
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Johnson, A.G., Han, I.H. (1974). Mitogenic Activity of Polynucleotides on Thymus-Influenced Lymphocytes. In: Braun, W., Lichtenstein, L.M., Parker, C.W. (eds) Cyclic AMP, Cell Growth, and the Immune Response. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-86026-3_7
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DOI: https://doi.org/10.1007/978-3-642-86026-3_7
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