Cyclic AMP and Microtubular Dynamics in Cultured Mammalian Cells

  • Theodore T. Puck
  • Carol Jones


The discovery, announced simultaneously by the laboratory of Dr. Pastan (1) and by ourselves (2) of profound change in cell habitus produced by dibutyryl cyclic AMP, appears to hold important implications for the field of cellular interactions generally and the nature of malignancy specifically. The reaction under consideration here resembles in many respects the changes produced when a cell is transformed to malignancy by an oncogenic virus or chemical agent. However, in contrast to the irreversible nature of the latter set of changes, that produced by cyclic AMP is completely reversible. It thus appears to furnish an ideal tool for study of the molecular nature of the underlying effects.


Wheat Germ Agglutinin Reverse Transformation Fibroblastlike Cell Chinese Hamster Cell Associational Recognition 
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  1. 1.
    Johnson, G. S., Friedman, R. M., and Pastan, I. “Restoration of Several Morphological Characteristics of Normal Fibroblasts in Sarcoma Cells Treated with Adenosine 3′,5′-Cyclic Monophosphate and Its Derivatives,” Proc. Nat. Acad. Sci. (U.S.A.) 68:425–429 (1971).CrossRefGoogle Scholar
  2. 2.
    Hsie, A. W., and Puck, T. T. “Morphological Transformation of Chinese Hamster Cells by Dibutyryl Adenosine Cyclic 3′,5′-Monophosphate and Testosterone,” Proc. Nat. Acad. Sci. (U.S.A.) 68:358–361 (1971).CrossRefGoogle Scholar
  3. 3.
    Hsie, A. W., Jones, C., and Puck, T. T. “Further Changes in Differentiation State Accompanying the Conversion of Chinese Hamster Cells to Fibroblastic Form by Dibutyryl Adenosine Cyclic 3′,5′-Monophosphate and Hormones,” Proc. Nat. Acad. Sci. (U.S.A.) 68:1648–1652 (1971).CrossRefGoogle Scholar
  4. 4.
    Puck, T. T., Waldren, C. A., and Hsie, A. W. “Membrane Dynamics in the Action of Dibutyryl Adenosine 3′,5′-Cyclic Monophosphate and Testosterone on Mammalian Cells,” Proc. Nat. Acad. Sci. (U.S.A.) 69:1943–1947 (1972).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1974

Authors and Affiliations

  • Theodore T. Puck
  • Carol Jones

There are no affiliations available

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