The Relationship between Swelling of Hard Tissue Collagen in Acid and Alkali and the Presence of Phosphate Cross-links

  • W. M. McKernan
  • S. D. Dailly
Conference paper

Abstract

While the swelling characteristics of soft tissue collagens have been extensively studied (Bowes and Kenten, 1950 a, b) only recently has attention been directed to similar studies of hard collagenous tissue. This latter work has arisen from the observation that the solubility of dentine collagen is very much less than that of corium collagen and this has been attributed to differences in the type or extent of intermolecular stabilisation in these two materials (Veis and Schlueter, 1963). More recently (Veisand Schlueter, 1964; Schlueter and Vets, 1964) it has been proposed that specific phosphate diester bonds are involved in cross-linking dentine collagen and that these esters are attached to the polypeptide chains through serine.

Keywords

Phosphorus Carbohydrate Adenosine Serine Polypeptide 

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References

  1. Bowes, J. H., and R. H. Kenten: The swelling of collagen in alkaline solutions. I. Swelling in solutions of sodium hydroxide. Biochem. J. 46, 1 (1950 a).Google Scholar
  2. Bowes, J. H., and R. H. Kenten: The swelling of collagen in alkaline solutions. II. Swelling in solutions of univalent bases. Biochem. J. 46, 524 (1950 b).Google Scholar
  3. Chayen, I. H., and D. R. Ashworth: The application of impulse rendering to the animal-fat industry. J. appl. Chem. 3, 529 (1953).CrossRefGoogle Scholar
  4. Michelson, O. B.: The determination of small concentrations of phosphate by ultraviolet spectrophotometry. Analyt. Chem. 1, 60 (1957).CrossRefGoogle Scholar
  5. Schlueter, R. J., and A. Vets: The macromolecular organisation of dentine matrix collagen.II. Periodate degradation and carbohydrate cross-linking. Biochemistry 3, 1657 (1964).PubMedCrossRefGoogle Scholar
  6. Veis, A., and R. J. Schlueter: Presence of phosphate-mediated cross-linkages in hard tissue collagens. Nature (Lond.) 197, 1204 (1963).Google Scholar
  7. Veis, A., and R. J. Schlueter: The macromolecular organisation of dentine matrix collagen. I. Characterisation of dentine collagen. Biochemistry 3, 1650 (1964).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1966

Authors and Affiliations

  • W. M. McKernan
    • 1
  • S. D. Dailly
    • 1
  1. 1.Research and Development Department, Gelatine DivisionBritish Glues and Chemicals Ltd.LondonEngland

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