Nucleoside, Nucleotide, Nucleinsäuren und Nucleoproteide

  • F. G. Fischer
  • H. Dörfel
Part of the Handbuch der Physiologisch- und Pathologisch-Chemischen Analyse book series (HOPPE-SEYLER, volume 4)

Zusammenfassung

Die Chemie der Nucleinsäuren und der Nucleoproteide ist in den Anfängen und schreitet daher sehr rasch fort. Die neueren analytischen Methoden, die Chromatographie in der Säule und auf dem Papierblatt, die Anwendung von Ionenaustauschern, die Ionophorese, im Verein mit den bequem gewordenen spektralphotometrischen Messungen, sind den Untersuchungen der Bestandteile dieser Stoffklassen besonders förderlich. Synthetische Verfahren haben in den letzten Jahren die künstliche Gewinnung der einfachen Nucleinsäuren-Spaltstücke nahezu vollständig ermöglicht und die Kenntnis ihrer Struktur gesichert. Die präparative Methodik zur Gewinnung der Proteide und der Säuren nutzt die Erfahrungen der Proteinchemie, insbesondere der Enzymisolierung, und erstrebt immer schonendere Darstellungsweisen. Die Anwendung der radioaktiven Isotopen des Phosphors, des Stickstoffs und des Kohlenstoffs gestattet es, die Reaktionsfolgen des Auf- und Abbaues der Nucleinsäuren und ihre Funktionen im Stoffwechsel zu verfolgen.

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