Advertisement

Bioassay of indolealkylamines

  • Vittorio Erspamer
Part of the Handbook of Experimental Pharmacology/Handbuch der experimentellen Pharmakologie book series (HEP, volume 19)

Abstract

For a satisfactory extraction of 5-HT from tissues it is sufficient that they be carefully cleaned, minced finely with scissors and then treated with 4 parts (w/v) of pure acetone, to give a final concentration of acetone of approximately 80%.

Keywords

Calcium Chloride Potassium Chloride Stimulant Action Helix Pomatia Smooth Muscle Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aixsworth, C.: Substituted β-aminoethylindazoles. J. Amer. chem. Soc. 80, 965–967 (1958).CrossRefGoogle Scholar
  2. Amix, A. H., T. B. B. Crawford and J. H. Gaddum: The distribution of substance P and 5-hydroxvtryptamine in the central nervous system of the dog. J. Physiol. (Lond.) 126, 596–618 (1954).Google Scholar
  3. Barlow, R. B., and I. Kahn: Actions of some analogues of tryptamine on the isolated rat uterus and on the isolated rat fundus strip preparations. Brit. J. Pharmacol. 14, 99–107 (1959 a).PubMedGoogle Scholar
  4. Barlow, R. B., and I. Kahn: Actions of some analogues of 5-hydroxytryptamine on the isolated rat uterus and the rat fundus strip preparations. Brit. J. Pharmacol. 14, 265–272 (1959 b).PubMedGoogle Scholar
  5. Barlow, R. B., and I. Kahn: The use of the guinea-pig ileum preparation for testing the activity of substances which imitate or antagonize the actions of 5-hydroxytryptamine and tryptamine. Brit. J. Pharmacol. 14, 553–558 (1959 c).PubMedGoogle Scholar
  6. Bexditt, E. P.,and R. L. Woxg: On the concentration of 5-hydroxytryptamine in mammalian enterochromaffin cells and its release by reserpine. J. exp. Med. 105, 509–519 (1957).CrossRefGoogle Scholar
  7. Bertaccixi, G., and P. Zamboxi: The relative potency of 5-hydroxytryptamine-like substances. Arch. int. Pharmacodyn. 133, 138–156 (1961).Google Scholar
  8. Boxxycastle, D. D., N. J. Giarmax, and M. K. Paasoxex: Anticonvulsant compounds and 5-hydroxytryptamine in rat brain. Brit. J. Pharmacol. 12, 228–231 (1957).Google Scholar
  9. Bowers jr., M. B.: Stimulation and sensitization of isolated Venus heart by cerebrospinal fluid. Brit, J. Pharmacol. 19, 295–298 (1962).Google Scholar
  10. Bumpus, F. M., and I. H. Page: Serotonin and its methylated derivatives in human urine. J. biol. Chem. 212, 111–116 (1955).PubMedGoogle Scholar
  11. Burdox, K. L., K. Ozkaragoz, H. S. Kaufman, and J. P. Mcgoverx: The effect of estrus on the response of the excised mouse uterus to serotonin, acetylcholine and specific antigen; Schultz-Dale tests with the physiograph. Ann. Allergy 18, 972–979 (1960).Google Scholar
  12. Burn, J. H.: Practical Pharmacology, p. 17. Oxford: Blackwell Sci. Publ. 1953.Google Scholar
  13. Busch, E.: Über die Erregbarkeit des Rattenuterus im Cyclus. Naunyn-Schmiedebergs Arch. exp. Path. Pharmakol. 228, 153–154 (1956).Google Scholar
  14. Cambridge, G. W., and J. A. Holgate: A method for identification of 5-hydroxytryptamine. J. Physiol. (Lond.) 130, 22P (1955).Google Scholar
  15. Cleugh, J., and J. H. Gaddum: The stability of purified preparations of substance P. Experientia (Basel) 19, 72–73 (1963).CrossRefGoogle Scholar
  16. Colhoun, E. H.: Synthesis of 5-hydroxytryptamine in the American cockroach. Experientia (Basel) 19, 9–10 (1963).CrossRefGoogle Scholar
  17. Correale, P.: The biological estimation of 5-hydroxytryptamine (enteramine) in the presence of substance P. J. Neurochem. 2, 201–208 (1958).PubMedCrossRefGoogle Scholar
  18. Dalgliesh, C. E., C. C. Toh, and T. S. Wobk: Fractionation of the smooth muscle stimulants present in extracts of the gastrointestinal tract. Identification of 5-hydroxytryptamine and its distinction from substance P. J. Physiol. (Lond.) 120, 298–310 (1953).Google Scholar
  19. Ebspameb, V.: Pharmakologische Studien über Enteramin. I. Über die Wirkung von Aceton-extrakten der Kaninchenmagenschleimhaut auf den Blutdruck und auf isolierte überlebende Organe. Naunyn-Schmiedebergs Arch. exp. Path. Pharmakol. 196, 343–365 (1940).CrossRefGoogle Scholar
  20. Ebspameb, V.: Active substances in the posterior salivary glands of octopoda. I. Enteramine-like substance. Acta pharmacol. (Kbh.) 4, 213–223 (1948).Google Scholar
  21. Ebspameb, V.: Il sistema cellulare enterocromaffine e l’enteramina (5-idrossitriptamina). Rend. sci. Farmitalia 1, 1–193 (1954).Google Scholar
  22. Ebspameb, V.: Observations of the fate of indolalkylamines in the organism. J. Physiol. (Lond.) 127, 118–133 (1955).Google Scholar
  23. Ebspameb, V.: and G. Falconieri Erspamer: Pharmacological actions of eledoisin on extravascular smooth muscle. Brit. J. Pharmacol. 19, 337–354 (1962).Google Scholar
  24. Ebspameb, V., and F. Ghiretti: The action of enteramine on the heart of molluscs. J. Physiol. (Lond.) 115, 470–481 (1951).Google Scholar
  25. Fänge, R.: Use of the isolated heart of a freshwater mussel (Anodonta cygnea L.) for biological estimation of 5-hydroxytryptamine. Experientia (Basel) 11, 156 (1955).CrossRefGoogle Scholar
  26. Feldberg, W., and C. C. Toh: Distribution of 5-hydroxytryptamine (serotonin, enteramine) in the wall of the digestive tract. J. Physiol. (Lond.) 119, 352–362 (1953).Google Scholar
  27. Fellman, J. H., T. S. Fujita, and C. J. Belbeb: 2,3-Dihydro-5-hydroxytryptamine. Biochem. Pharmacol. 11, 557–561 (1962).PubMedCrossRefGoogle Scholar
  28. Fenster, E., and J. C. Towne: Solubilization of serotonin by rat brain extracts in lipid solvents. Fed. Proc. 22, 624 (1963).Google Scholar
  29. Gaddum, J. H.: The technique of superfusion. Brit. J. Pharmacol. 8, 321–326 (1953).PubMedGoogle Scholar
  30. Gaddum, J. H., and K. A. Hameed: Drugs which antagonize 5-hydroxytryptamine. Brit. J. Pharmacol. 9, 240–248 (1954).PubMedGoogle Scholar
  31. Gaddum, J. H., and M. K. Paasonen: The use of some molluscan hearts for the estimation of 5-hydroxytryptamine. Brit. J. Pharmacol. 10, 474–483 (1955).PubMedGoogle Scholar
  32. Garven, J. D.: The estimation of 5-hydroxytryptamine in the presence of adrenaline. Brit. J. Pharmacol. 11, 66–70 (1956).PubMedGoogle Scholar
  33. Greenberg, M. J.: The response of the Venus heart to catechol amines and high concentrations of 5-hydroxytryptamine. Brit. J. Pharmacol. 15, 365–374 (1960a).PubMedGoogle Scholar
  34. Greenberg, M. J.: Structure-activity relationship of tryptamine analogues on the heart of Venus mercenaria. Brit. J. Pharmacol. 15, 375–388 (1960b).PubMedGoogle Scholar
  35. Grette, K.: Determination of 5-hydroxytryptamine with the isolated, perfused pig ear. Acta pharmacol. (Kbh.) 13, 177–183 (1957).CrossRefGoogle Scholar
  36. Gryglewski, R., and J. Supniewski: Influence of 5-hydroxytryptamine and other biologically active substances on the movements of the isolated stomach of Helix pomatia Bull. Acad. pol. Sci., Ser. Sci. Biol. 11, 53–56 (1963).Google Scholar
  37. Haefeli, W., and A. Hürlimann: Substance P, a highly active naturally occurring polypeptide. Experientia (Basel) 18, 297–303 (1962).CrossRefGoogle Scholar
  38. Kerkut, G. A., and G. A. Cottrell: Acetylcholine and 5-hydroxytryptamine in the snail brain. Comp. Biochem. Physiol. 8, 53–63 (1963).CrossRefGoogle Scholar
  39. Leitsch, J. L., D. Maryn and J. B. Moore: Note on sensitivity of the isolated perfused rat uterus to serotonin. J. Amer. pharm. Ass. (Sci. Ed.) 47, 535 (1958).CrossRefGoogle Scholar
  40. Marczynski, T.: Preliminary investigations of the pharmacological properties of 5-methoxy-N-methyltryptamine. The freshwater crustacean Anodonta cygnea L. as a test for serotonin and related compounds. Diss. pharm. (Krakow) 11, 297–313 (1959).Google Scholar
  41. Mcgovern, J. P., K. Ozkaragoz, A.E. Hensel, and K.L. Burdon: Qualitative and quantitative studies with 5-hydroxytryptamine (serotonin) in the Schultz-Dale apparatus. J. Allergy 32, 321–326 (1961).PubMedCrossRefGoogle Scholar
  42. Mcintyre, A.D., D.E. Williams, and F. L. Humoller: Bioassay of 5-hydroxytryptamine on heart of Venus mercenaria. Fed. Proc. 19, 283 (1960).Google Scholar
  43. Mcisaac., W. M., and I. H. Page: New metabolites of serotonin in carcinoid urine. Science 128, 537 (1958).PubMedCrossRefGoogle Scholar
  44. Osborne, M., R. Detar, and L. Gabel: Comparison between the autonomic pharmacology of 5-hydroxytryptamine and 5-acetyltryptamine (5 AT). Fed. Proc. 21, 324 (1962).Google Scholar
  45. Page, I. H., and A. A. Green: Perfusion of rabbit’s ear for study of vasoconstrictor substances. Meth. med. Res. 1, 123–129 (1948).PubMedGoogle Scholar
  46. Poloni, A.: Il muscolo dorsale di sanguisuga quale test biologico per l’evidenziamento del-l’attività serotoninica nei liquidi organici. Cervello 31, 472–476 (1955).Google Scholar
  47. Put, T. R., and L. A. H. Hogenhuis: Brain serotonin and thyroid function. Acta physiol. pharmacol. neerl. 10, 343–352 (1962).PubMedGoogle Scholar
  48. Ravenna, F.: Sintesi e attività biologica del 5-ammino-triptofano. Experientia (Basel) 14, 273–274 (1958).CrossRefGoogle Scholar
  49. Renson, J., H. Weissbach, and S. Udenfriend: Studies on the biological activities of the aldehydes derived from norepinephrine, serotonin, tryptamine and histamine. J. Pharmacol. exp. Ther. 143, 326–331 (1964).PubMedGoogle Scholar
  50. Robson, J. M., Trounce, and K. A. H. Didcock: Factors affecting the response of the uterus to serotonin. J. Endocr. 10, 129–132 (1954).PubMedCrossRefGoogle Scholar
  51. Schain, R. J.: Effects of 5-hydroxytryptamine on the dorsal muscle of the leech (Hirudo medicinalis). Brit. J. Pharmacol. 16, 257–261 (1961).PubMedGoogle Scholar
  52. Schötensack, W., P. Bischler u. G. Richarz: Vergleich pharmakologischer Wirkungen von Indol-und Benzofuran-alkylaminen. Naunyn-Schmiedebergs Arch. exp. Path. Pharma-kol. 245, 120–121 (1963).CrossRefGoogle Scholar
  53. Schwartz, I. L.: Assay of serotonin with antimetabolites. Amer. J. Physiol. 183, 659–660 (1955).Google Scholar
  54. Supek, Z., and S. Milkovic: Quantitative biological determination of 5-hydroxytryptamine. Experientia (Basel) 12, 71–72 (1956).CrossRefGoogle Scholar
  55. Supek, Z., and M. Randic: An improved method for biological determination of 5-hydroxytryptamine in acetone brain extracts in the presence of interfering substances. Biochem. Pharmacol. 8, 79 (1961).CrossRefGoogle Scholar
  56. Towne, J. C.,and PH. K. Kalayil: Lipid solvent-amine interaction. Fed. Proc. 23, 492 (1964).Google Scholar
  57. Uuspää, V. J., and V. I. Uuspää: Vane’s rat fundus strip method in determination of 5-hydroxytryptamine. Ann. Acad. Sci. fenn. A IV, 60, 1–17 (1962).Google Scholar
  58. Vane, J. R.: A sensitive method for the assay of 5-hydroxytryptamine. Brit. J. Pharmacol. 12, 344–349 (1957).PubMedGoogle Scholar
  59. Vane, J. R.: The relative activities of some tryptamine analogues on the isolated rat stomach strip preparation. Brit. J. Pharmacol. 14, 87–98 (1959).PubMedGoogle Scholar
  60. Welsh, J. H.: Neurohormones of invertebrates. I. Cardio-regulators of Cyprina and Buccinum. J. Mar. Biol. Ass. U. Kingd. 35, 193–201 (1956).CrossRefGoogle Scholar
  61. Vane, J. R.: Serotonin as a possible neurohumoral agent: evidence obtained in lower animals. Ann. N. Y. Acad. Sci. 66, 618–630 (1957).CrossRefGoogle Scholar
  62. Vane, J. R., and R. Taub: The action of choline and related compounds on the heart of Venus mercenaria. Biol. Bull. 95, 346–353 (1948).CrossRefGoogle Scholar
  63. Woolley, D. W.: Highly potent antimetabolites of serotonin with little serotonin-like action. Biochem. Pharmacol. 1, 51–59 (1959).CrossRefGoogle Scholar
  64. Woolley, D. W., and N. K. Campbell: Serotonin-like and antiserotonin properties of psilocybin and psilocin. Science 136, 777–779 (1962).PubMedCrossRefGoogle Scholar
  65. Zetler, G., u. L. Schlosser: Über das Vorkommen von 5-Hydroxytryptamin (Enteramin oder Serotonin) im Gehirn von Säugetieren. Naunyn-Schmiedebergs Arch. exp. Path. Pharmakol. 222, 345–351 (1954).Google Scholar

Copyright information

© Springer-Verlag, Berlin · Heidelberg 1966

Authors and Affiliations

  • Vittorio Erspamer

There are no affiliations available

Personalised recommendations