Abstract
The protein fraction of milk consists of a few major lactoprotein species synthesized in the lactating mammary gland. In bovine milk caseins represent 80% of the total lactoproteins. Linkage studies [1, 2] and in situ hybridization [3, 5] revealed linkage betweenα s1-Cn-α s2-Cn-ß-Cn-ϰ-Cn genes, forming a gene cluster on a single chromosome, controlled by steroid and peptide hormones. Large amounts of casein-specific mRNAs in the lactating mammary gland make possible the successful application of recombinant DNA techniques to establish cDNA libraries [9]. Isolation of genomic sequences, coding for caseins, sequencing of cDNA and genomic DNA clones, and RFLP studies offer much information about the fine structure and possible mechanisms of regulation of that gene complex. In the following experiments we studied expression of the α s1-casein-coding cDNA clone using two in vitro expression systems.
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References
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© 1989 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt
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Niepold, F., Dovč, P., Rottmann, O.J. (1989). Expression of an α s1,-Casein cDNA-Clone in a Cell-free and Procaryote Model System. In: Barth, C.A., Schlimme, E. (eds) Milk Proteins. Steinkopff. https://doi.org/10.1007/978-3-642-85373-9_22
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DOI: https://doi.org/10.1007/978-3-642-85373-9_22
Publisher Name: Steinkopff
Print ISBN: 978-3-642-85375-3
Online ISBN: 978-3-642-85373-9
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