Milk Proteins and Metabolic Requirements of Trace Elements, Minerals, and Vitamins

  • B. Lönnerdal


For most age groups, milk is considered an excellent source of many nutrients. Even those nutrients which do not occur in high concentrations in milk appear to be well taken up from milk and dairy products. When examining the reasons for the high bioavailability of nutrients from milk, a distinction must be made between the newborn infant with a not yet fully developed gastrointestinal tract and the adult with a much higher capacity for digestion. It is likely that the mechanisms explaining the high bioavailability will be quite different for these two age groups. In this paper, I will therefore attempt to give this developmental perspective to nutrient absorption from milk. Since many nutrients are bound to proteins, this discussion will primarily focus on milk proteins and how they differ from other sources of protein.


Iron Absorption Human Milk Zinc Absorption Milk Protein Bovine Lactoferrin 
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  1. 1.
    Antony AC, Utley CS, Marcell PD, Kolhouse JF (1982) Isolation, characterization, and comparison of the solubilized particulate and soluble folate binding proteins from human milk. J Biol Chem 257: 10081–10089PubMedGoogle Scholar
  2. 2.
    Barton JC, Conrad ME, Parmley RT (1983) Calcium inhibition of inorganic iron absorption in rats. Gastroenterology 84: 90–91PubMedGoogle Scholar
  3. 3.
    Bell JG, Keen CL, Lönnerdal B (1987) Effect of infant cereals on zinc and copper absorption during weaning. Am J Dis Child 141: 1128–1132PubMedGoogle Scholar
  4. 4.
    Burger RL, Allen RH (1974) Characterization of vitamin B12-binding proteins from human milk and saliva by affinity chromatography. J Biol Chem 249: 7220–7227PubMedGoogle Scholar
  5. 5.
    Casey CE, Walravens PA, Hambidge KM (1981) Availability of zinc: loading tests with human milk, cow’s milk, and infant formulas. Pediatrics 68: 394–396PubMedGoogle Scholar
  6. 6.
    Cochet BA, Juny M, Griessen P, Bartholdi P, Schaller P, Donath A (1983) Effects of lactose on intestinal calcium absorption in normal and lactase-deficient subjects. Gastroenterology 84: 935–940PubMedGoogle Scholar
  7. 7.
    Colman N, Hettiarachchy N, Herbert V (1981) Detection of a milk factor that facilitates folate uptake by intestinal cells. Science 211: 1427–1429PubMedCrossRefGoogle Scholar
  8. 8.
    Cox TM, Mazurier J, Spik G, Montreuil J, Peters TJ (1979) Iron-binding proteins and influx of iron across the duodenal brush border. Biochim Biophys Acta 588: 120–128PubMedCrossRefGoogle Scholar
  9. 9.
    Craig WJ, Balbach L, Harris S, Vyhmeister N (1984) Plasma zinc and copper levels of infants fed different milk formulas. J Am Coll Nutr 3: 183–186PubMedGoogle Scholar
  10. 10.
    Davidson LA, Lönnerdal B (1988) Specific binding of lactoferrin to brush border membrane: ontogeny and effect of glycan chain. Am J Physiol 254: G580 — G585PubMedGoogle Scholar
  11. 11.
    Fairweather-Tait SJ, Balmer SE, Scott PH, Minski MJ (1988) Lactoferrin and iron absorption in newborn infants. Pediatr Res 22: 651–654CrossRefGoogle Scholar
  12. 12.
    Ford JE, Salter DN, Scott KJ (1969) The folate-binding protein in human milk. J Dairy Res 36:435–446Google Scholar
  13. 13.
    Fransson G-B, Lönnerdal B (1983) Distribution of trace elements and minerals in human and cow’s milk. Pediatr Res 17: 912–915PubMedCrossRefGoogle Scholar
  14. 14.
    Fransson G-B, Keen CL, Lönnerdal B (1983) Supplementation of milk with iron bound to lactoferrin using weanling mice. I. Effects on hematology and tissue iron. J Pediatr Gastroenterol Nutr 2: 693–700Google Scholar
  15. 15.
    Fransson G-B, Thoren-Tolling K, Jones B, Hambraeus L, Lönnerdal B (1983) Absorption of lactoferrin-iron in suckling pigs. Nutr Res 3: 373–384CrossRefGoogle Scholar
  16. 16.
    Ghishan FK (1984) Transport of electrolytes, water and glucose in zinc deficiency. J Pediatr Gastroenterol Nutr 3: 608–612PubMedCrossRefGoogle Scholar
  17. 17.
    Gillooly M, Bothwell TH, Torrance JD, MacPhail AP, Derman DP et al. (1983) The effects of organic acids, phytates, and polyphenols on the absorption of iron from vegetables. Br J Nutr 49: 331— 342Google Scholar
  18. 18.
    Gillooly M, Torrance JD, Bothwell TH, MacPhail AP, Derman D et al. (1984) The relative effect of ascorbic acid on iron absorption from soy-based and milk-based infant formulas. Am J Clin Nutr 40: 522–527PubMedGoogle Scholar
  19. 19.
    Hallberg L, Rossander L, Skanberg A-B (1987) Phytates and the inhibitory effect of bran on iron absorption in man. Am J Clin Nutr 45: 988–996PubMedGoogle Scholar
  20. 20.
    Hambidge KM, Walravens PA, Casey CE, Brown RM, Bender C (1979) Plasma zinc concentrations of breast-fed infants. J Pediatr 94: 607–608PubMedCrossRefGoogle Scholar
  21. 21.
    Hazell T, Johnson IT (1987) In vitro estimation of iron availability from a range of plant foods: influence of phytate, ascorbate and citrate. Br J Nutr 57: 223–233PubMedCrossRefGoogle Scholar
  22. 22.
    Hegenauer J, Saltman P, Ludwig D, Ripley L, Ley A (1979) Iron-supplemented cow milk. Identification and spectral properties of iron bound to casein micelles. J Agric Food Chem 27: 1294–1301Google Scholar
  23. 23.
    Kiely J, Flynn A, Singh H, Fox PF (1988) Improved zinc bioavailability from colloidal calcium phosphate-free cow’s milk. In: Hurley LS, Keen CL, Lönnerdal B, Rucker RB (eds) Trace Elements in Man and Animals — 6, Plenum Press, New York, in pressGoogle Scholar
  24. 24.
    Lebenthal E, Lee PC, Heitlinger LA (1983) Impact of development of the gastrointestinal tract on infant feeding. J Pediatr 102: 1–9PubMedCrossRefGoogle Scholar
  25. 25.
    Lee YS, Noguchi T, Naito H (1980) Phosphopeptides and soluble calcium in the small intestine of rats given a casein diet. Br J Nutr 43: 457–467PubMedCrossRefGoogle Scholar
  26. 26.
    Lee YS, Noguchi T, Naito H (1983) Absorption of calcium in rats given diets containing casein or amino acid mixture: the role of casein phosphopeptides. Br J Nutr 49: 67–76PubMedCrossRefGoogle Scholar
  27. 27.
    Llanes TE, Lönnerdal B (1987) Digestibility of human, bovine and Rhesus monkey milk. Fed Proc 46: 895Google Scholar
  28. 28.
    Lönnerdal B (1984) Iron in breast milk. In: Stekel A (ed) Iron Nutrition in Infancy and Childhood. Nestle Nutrition Workshop Series 4: 95–118. Vevey/Raven Press, New YorkGoogle Scholar
  29. 29.
    Lönnerdal B (1985) Dietary factors affecting trace element bioavailability from human milk, cow’s milk and infant formulas. Progress in Food and Nutrition Science 9:35–62. Pergamon Press, OxfordGoogle Scholar
  30. 30.
    Lönnerdal B, Glazier C (1988) An approach to assessing trace element bioavailability from milk in vitro: extrinsic labeling and proteolytic degradation. Biol Trace Element Res (in press)Google Scholar
  31. 31.
    Lönnerdal B, Stanislowski AG, Hurley LS (1980) Isolation of a low molecular weight zinc binding ligand from human milk. J Inorg Biochem 12: 71–78PubMedCrossRefGoogle Scholar
  32. 32.
    Lönnerdal B, Cederblad A, Davidsson L, Sandström B (1984) The effect of individual components of soy formula and cow’s milk formula on zinc bioavailability. Am J Clin Nutr 40: 1064–1070PubMedGoogle Scholar
  33. 33.
    Lönnerdal B, Keen CL, Bell JG, Hurley LS (1985) Zinc uptake and retention from chelates and milk fractions. In: Mills CF, Bremner I, Chesters JK (eds) Trace elements in Man and Animals — TEMA 5. Commonwealth Agricultural Bureaux, Farnham Royal, UKGoogle Scholar
  34. 34.
    Lönnerdal B, Bell JG, Hendrickx AG, Burns RA, Keen CL (1988) Effect of phytate removal on zinc absorption from soy formula. Am J Clin Nutr (in press)Google Scholar
  35. 35.
    Mason JB, Selhub J (1988) Folate-binding protein and the absorption of folic acid in the small intestine of the suckling rat. Am J Clin Nutr 48: 620–625PubMedGoogle Scholar
  36. 36.
    McMillan JA, Landaw SA, Oski FA (1976) Iron sufficiency in breast-fed infants and the availability of iron from human milk. Pediatrics 58: 686–691PubMedGoogle Scholar
  37. 37.
    McMillan JA, Oski FA, Lourie G, Tomarelli RM, Landaw SA (1977) Iron absorption from human milk, simulated human milk, and proprietary formulas. Pediatrics 60: 896–900PubMedGoogle Scholar
  38. 38.
    Mellander 0 (1950) The physiologic importance of the casein phosphopeptide calcium salt. II. Peroral calcium dosage of infants. Acta Soc Med Upsal 55: 247–255Google Scholar
  39. 39.
    Morck TA, Lynch SR, Skikne BS, Cook JD (1981) Iron availability from infant food supplements. Am J Clin Nutr 34: 2630–2634PubMedGoogle Scholar
  40. 40.
    Mykkänen HM, Wasserman RH (1980) Enhanced absorption of calcium by casein phosphopeptides in rachitic and normal chicks. J Nutr 110: 2141–2148PubMedGoogle Scholar
  41. 41.
    Nicar MJ, Pak CYC (1985) Calcium bioavailability from calcium carbonate and calcium citrate. J Clin Endocrinol Metab 61: 391–393PubMedCrossRefGoogle Scholar
  42. 42.
    Recker RR, Bammi A, Barger-Lux J, Heaney RP (1988) Calcium absorbability from milk products, an imitation milk, and calcium carbonate. Am J Clin Nutr 47: 93–95PubMedGoogle Scholar
  43. 43.
    Rolston DDK, Moriarty KJ, Farthing MJG, Kelly MJ, Clark ML, Dawson AM (1986) Acetate and citrate stimulate water and sodium absorption in the human jejunum. Digestion 34: 101–104PubMedCrossRefGoogle Scholar
  44. 44.
    Rowe J, Rowe D, Horak D et al. (1984) Hypophosphatemia and hypercalciuria in small premature infants fed human milk. J Pediatr 104: 112–117PubMedCrossRefGoogle Scholar
  45. 45.
    Saarinen UM, Siimes MA, Dallman PR (1977) Iron absorption in infants: High bioavailability of breast milk iron as indicated by the extrinsic tag method of iron absorption and by the concentration of serum ferritin. J Pediatr 91: 36–39Google Scholar
  46. 46.
    Said HM, Home DW, Wagner C (1985) Effect of human milk folate binding protein on folate intestinal transport. Arch Biochem Biophys 251: 114–120CrossRefGoogle Scholar
  47. 47.
    Salter DN, Blakeborough P (1988) Influence of goat’s-milk folate-binding protein on transport of 5-methyltetrahydrofolate in neonatal-goat small intestinal brush-border-membrane vesicles. Br J Nutr 59: 497–507PubMedCrossRefGoogle Scholar
  48. 48.
    Salter DN, Mowlem A (1983) Neonatal role of milk folate-binding protein: studies on the course of digestion of goat’s milk folate binder in the 6-d-old kid. Br J Nutr 50: 589–596PubMedCrossRefGoogle Scholar
  49. 49.
    Sandberg DP, Begley JA, Hall CA (1981) The content, binding, and forms of vitamin B12 in milk. Am J Clin Nutr 34: 1717–1724PubMedGoogle Scholar
  50. 50.
    Sandström B, Arvidsson B, Cederblad A, Björn-Rasmussen E (1980) Zinc absorption from composite meals. I. The significance of wheat extraction rate, zinc, calcium, and protein content in meals based on bread. Am J Clin Nutr 33: 739–745Google Scholar
  51. 51.
    Sandström B, Cederblad A, Lönnerdal B (1983) Zinc absorption from human milk, cow’s milk and infant formulas. Am J Dis Child 137: 726–729PubMedGoogle Scholar
  52. 52.
    Sandström B, Keen CL, Lönnerdal B (1983) An experimental model for studies of zinc bio-availability from milk and infant formulas using extrinsic labelling. Am J Clin Nutr 38: 420–428PubMedGoogle Scholar
  53. 53.
    Sandström B, Davidsson L, Cederblad A, Lönnerdal B (1985) Oral iron, dietary ligands and zinc absorption. J Nutr 115: 411–414PubMedGoogle Scholar
  54. 54.
    Sato R, Noguchi T, Naito H (1986) Casein phosphopeptide ( CPP) enhances calcium absorption from the ligated segment of rat small intestine. J Nutr Sci Vitaminol 32: 67–76Google Scholar
  55. 55.
    Sczekan SR, Joshi JG (1987) Isolation and characterization of ferritin from soyabeans ( Glycine max ). J Biol Chem 262: 13780–13788Google Scholar
  56. 56.
    Seal CJ, Heaton FW (1983) Chemical factors affecting the intestinal absorption of zinc in vitro and in vivo. Br J Nutr 50: 317–324PubMedCrossRefGoogle Scholar
  57. 57.
    Senterre J, Salle B (1982) Calcium and phosphorus economy of the preterm infant and its interaction with vitamin D and its metabolites. Acta Paediatr Scand Suppl 296: 85–92PubMedCrossRefGoogle Scholar
  58. 58.
    Smith TM, Kolars JC, Savino DA, Levitt MD (1985) Absorption of calcium from milk and yogurt. Am J Clin Nutr 42: 1197–1200PubMedGoogle Scholar
  59. 59.
    Solomons NW, Jacob RA (1981) Studies on the bioavailability of zinc in humans: effect of heure and nonheme iron on the absorption of zinc. Am J Clin Nutr 34: 475–482PubMedGoogle Scholar
  60. 60.
    Spik G, Strecker G, Fournet B, Bouquelet S, Montreuil J (1982) Primary structure of the glycans from human lactotransferrin. Eur J Biochem 121: 413–419PubMedCrossRefGoogle Scholar
  61. 61.
    Trugo NMF, Newport MJ (1985) Vitamin B12 absorption in the neonatal piglet. 2. Resistance of the vitamin B12-binding protein in sows’ milk to proteolysis in vivo. Br J Nutr 54: 257–267PubMedCrossRefGoogle Scholar
  62. 62.
    Trugo NMF, Ford JE, Sansom BF (1985) Vitamin B12 absorption in the neonatal piglet. 1. Studies in vivo on the influence of the vitamin B12-binding protein from sow’s milk on the absorption of vitamin B12 and related compounds. Br J Nutr 54: 245–255PubMedCrossRefGoogle Scholar
  63. 63.
    Trugo NMF, Ford JE, Salter DN (1985) Vitamin B12 absorption in the neonatal piglet. 3. Influence of vitamin B12-binding protein from sows’ milk on uptake of vitamin B12 by microvillous membrane vesicles prepared from small intestine of the piglet. Br J Nutr 54: 269–283PubMedCrossRefGoogle Scholar
  64. 64.
    Ziegler EE, Figueroa-Colón R, Serfass RE, Nelson SE (1987) Effect of low dietary zinc on zinc metabolism in infancy: stable isotope studies. Am J Clin Nutr 45: 849Google Scholar
  65. 65.
    Ziegler EE, Fomon SJ (1983) Lactose enhances mineral absorption in infancy. J Pediatr Gastroenterol Nutr 2: 288–294PubMedGoogle Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1989

Authors and Affiliations

  • B. Lönnerdal
    • 1
  1. 1.Department of NutritionUniversity of CaliforniaDavisUSA

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