Zine insulin  (1.2 g, 0.2 mmol) is dissolved in 0.025 N HCl and dialyzed at 0 ° for 5 days , with frequent changes of the 0.025 N HCl. Lyophilization of the content of the dialysis bag leaves insulin hydrochloride (1.2 g) which has a zinc content of about 0.04% .
KeywordsHuman Insulin Zinc Content Porcine Insulin Butyl Ether Chloromethyl Ketone
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