New tools for the study of chromosome segregation and aneuploidy at the molecular level

  • J.-P. Charlieu
  • B. Marçais
  • A.-M. Laurent
  • G. Roizès
Conference paper
Part of the NATO ASI Series book series (volume 72)

Abstract

The molecular mechanisms which allow the correct distribution of chromosomes during cell division are not yet well known. The centromere, because of its possible involvement in the attachment of sister chromatids and its participation in the formation of the kinetochore, may play an important role in these mechanisms. Trisomy 21 (Down syndrome, DS) provides a good model for studying aneuploidy resulting from the dysfunction of the chromosome distribution process. A possible means of analyzing the mechanisms leading to nondisjunction (NDJ) could be to determine the origin of the supernumerary chromosome 21 and to attempt to find some structural or physical characteristics of the potentially responsible centromere. This could be performed by using molecular tools which allow each of the two parental chromosomes 21 to be distinguished. Possible markers suitable for this purpose are DNA fragments that exhibit length polymorphisms.

Keywords

Hydrolysis Recombination Agarose Electrophoresis Polyacrylamide 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • J.-P. Charlieu
    • 1
  • B. Marçais
    • 1
  • A.-M. Laurent
    • 1
  • G. Roizès
    • 1
  1. 1.Institut de BiologieCNRS UPR 9008 & INSERM U 249Montpellier cedexFrance

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