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Regulation of the Human Neurofilament Light and Heavy Genes in Transgenic Mice

  • G. Charron
  • L. Beaudet
  • E. Côté
  • D. Houle
  • J.-P. Julien
Conference paper
Part of the Research and Perspectives in Neurosciences book series (NEUROSCIENCE)

Summary

Using the transgenic mouse approach, we investigated the mechanisms that regulate expression of the human neurofilament light (NF-L) and heavy (NF-H) genes. Both human NF-L and NF-H genes are expressed in a tissue-specific fashion in transgenic mice. For the NF-L gene, various constructs have been tested in transgenic mice to define regulatory regions contributing to neuronal expression. Unexpectedly, this analysis demonstrated the presence of intragenic elements in the NF-L gene that confer cell-type specific regulation on the basal activity of the proximal NF-L promoter. Moreover, the examination of transgenic mice bearing a high-copy number of the NF-L gene revealed the existence of a translational control impeding overexpression of NF-L in CNS neurons. Unlike the NF-L gene, overexpression of the human NF-H gene in transgenic mice was not impeded by the existence of such posttranscriptional controls.

Keywords

Chloramphenicol Acetyl Transferase Spinal Cord Sample Chloramphenicol Acetyl Transferase Activity Heavy Gene Central Nervous Sytem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • G. Charron
  • L. Beaudet
  • E. Côté
  • D. Houle
  • J.-P. Julien

There are no affiliations available

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