Regulation of the Human Neurofilament Light and Heavy Genes in Transgenic Mice
Using the transgenic mouse approach, we investigated the mechanisms that regulate expression of the human neurofilament light (NF-L) and heavy (NF-H) genes. Both human NF-L and NF-H genes are expressed in a tissue-specific fashion in transgenic mice. For the NF-L gene, various constructs have been tested in transgenic mice to define regulatory regions contributing to neuronal expression. Unexpectedly, this analysis demonstrated the presence of intragenic elements in the NF-L gene that confer cell-type specific regulation on the basal activity of the proximal NF-L promoter. Moreover, the examination of transgenic mice bearing a high-copy number of the NF-L gene revealed the existence of a translational control impeding overexpression of NF-L in CNS neurons. Unlike the NF-L gene, overexpression of the human NF-H gene in transgenic mice was not impeded by the existence of such posttranscriptional controls.
KeywordsChloramphenicol Acetyl Transferase Spinal Cord Sample Chloramphenicol Acetyl Transferase Activity Heavy Gene Central Nervous Sytem
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