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Detection of Human T-cell Leukemia Virus Type 1 Provirus: Semiquantitative, Nested, Double Polymerase Chain Reaction

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Diagnosis of Human Viruses by Polymerase Chain Reaction Technology

Part of the book series: Frontiers of Virology ((FRVIROLOGY,volume 1))

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Summary

The polymerase chain reaction (PCR) is a powerful tool for diagnosing human T-cell leukemia virus type 1 (HTLV-I) infection, particularly for samples difficult to assess by immunological methods. We used the nested double PCR method to detect HTLV-I provirus in DNA obtained from an infected cell line and peripheral blood mononuclear cells from donated blood. This method yielded clear results even if only one template DNA was amplified. Furthermore, the amplified products showed one, two, or three bands after electro-phoresis, depending on the amounts of template DNA present. The semiquantitative, nested, double PCR and its applications are described in this chapter.

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Authors and Affiliations

  1. The Japanese Red Cross Central Blood Center, Hiroo 4-1-31, Shibuya-ku, Tokyo, 150, Japan

    Chieko Matsumoto & Kusuya Nishioka

Authors
  1. Chieko Matsumoto
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  2. Kusuya Nishioka
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Editors and Affiliations

  1. Department of Molecular Virology Faculty of Medicine, Hebrew University of Jerusalem, POB 1172, Jerusalem, Israel

    Yechiel Becker

  2. Institut für Medizinische Virologie, der Universität Heidelberg, Im Neuenheimer Feld 324, W-6900, Heidelberg, Germany

    Gholamreza Darai

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© 1992 Springer-Verlag Berlin Heidelberg

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Matsumoto, C., Nishioka, K. (1992). Detection of Human T-cell Leukemia Virus Type 1 Provirus: Semiquantitative, Nested, Double Polymerase Chain Reaction. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_3

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  • DOI: https://doi.org/10.1007/978-3-642-84766-0_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84768-4

  • Online ISBN: 978-3-642-84766-0

  • eBook Packages: Springer Book Archive

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