Detection of Human T-cell Leukemia Virus Type 1 Provirus: Semiquantitative, Nested, Double Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a powerful tool for diagnosing human T-cell leukemia virus type 1 (HTLV-I) infection, particularly for samples difficult to assess by immunological methods. We used the nested double PCR method to detect HTLV-I provirus in DNA obtained from an infected cell line and peripheral blood mononuclear cells from donated blood. This method yielded clear results even if only one template DNA was amplified. Furthermore, the amplified products showed one, two, or three bands after electro-phoresis, depending on the amounts of template DNA present. The semiquantitative, nested, double PCR and its applications are described in this chapter.
KeywordsSingle Polymerase Chain Reaction Amplification Stage Particle Agglutination Tropical Spastic Paraparesis Repeated Polymerase Chain Reaction
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