Polymerase Chain Reaction for the Detection of Adenoviruses
The use of the polymerase chain reaction (PCR) for the detection of human adenoviruses in diluted stool samples was investigated. Several primers, including ones specific for the hexon-coding region and for enteric adenovirus (EAd) types 40 and 41, were evaluated. The primers constitute three different PCR systems designed for the detection of all six adenovirus subgenera (A-F), the two EAds Ad40 and Ad41, and Ad40, respectively. The general system and the EAd-specific system also include nested primers for a two-step amplification. The limit of detection of the PCR assay in a two-step amplification was 1 single virus particle when the two sets of general hexon primers or EAd-specific primers were used. The sensitivity of the PCR, together with its simplicity and reduced time-scale compared with other detection methods, emphasize the potential of this technique as an additional method for routine diagnosis of human adenovirus infections.
KeywordsStool Sample Stool Specimen Hemorrhagic Cystitis Adenovirus Type Human Adenovirus
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