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Semiquantitative Analysis of Epstein-Barr Virus DNA by Polymerase Chain Reaction in Clinical Samples of Lymphoproliferative Disorders

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Diagnosis of Human Viruses by Polymerase Chain Reaction Technology

Part of the book series: Frontiers of Virology ((FRVIROLOGY,volume 1))

Summary

Epstein-Barr virus (EBV) DNA was detected and semiquantitatively analyzed in clinical samples from patients with Hodgkin’s disease (HD), angioim-munoblastic lymphadenopathy (AILD), hairy cell leukemia (HCL), T-lymphoblastic lymphoma (TLL), familial gastric lymphoma, lymphocytic/ mixed thymoma, and reactive lymph node hyperplasia (HR). High numbers of viral DNA copies were detected in HD and AILD, whereas no or very few viral DNA copies were found in HCL and thymoma. One case of TLL and two cases of HR contained relatively high numbers of EBV DNA copies. In the case of TLL, the viral genomes probably originated from accessory B cells. The high amount of EBV DNA identified in the HD and AILD cases is not associated with the Bam W/Bam Z rearrangement known to disrupt viral latency. Technical details of amplification, oligonucleotide hybridization, and semiquantitative EBV DNA analysis are described. A sensitive detection system of the Bam W/Bam Z rearrangement is also presented.

This study was supported in part by grants no. 31-26389.89 and no. 31-30865.91 from the Swiss Natlonal Foundation. D. J. L. I is a recipient of the PF Sobotka postgraduate research fellowship of the University of Western Australia.

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Authors and Affiliations

  1. Department of Medicine and Institutes, University Hospital CHUV, Lausanne, Switzerland

    Hans Knecht, David J. L. Joske, Edith Bachmann, Fedor Bachmann & Daniel Hayoz

  2. Department of Medicine and Institutes of Microbiology, University Hospital CHUV, Lausanne, Switzerland

    Roland Sahli

  3. Department of Medicine and Institutes of Pathology, University Hospital CHUV, Lausanne, Switzerland

    Phil Shaw

  4. Department of Pathology, University Hospital, Zürich, Switzerland

    Bernhard F. Odermatt

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  1. Hans Knecht
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  2. Roland Sahli
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  3. David J. L. Joske
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  4. Edith Bachmann
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  5. Fedor Bachmann
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  6. Daniel Hayoz
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  7. Bernhard F. Odermatt
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  8. Phil Shaw
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Editors and Affiliations

  1. Department of Molecular Virology Faculty of Medicine, Hebrew University of Jerusalem, POB 1172, Jerusalem, Israel

    Yechiel Becker

  2. Institut für Medizinische Virologie, der Universität Heidelberg, Im Neuenheimer Feld 324, W-6900, Heidelberg, Germany

    Gholamreza Darai

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© 1992 Springer-Verlag Berlin Heidelberg

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Knecht, H. et al. (1992). Semiquantitative Analysis of Epstein-Barr Virus DNA by Polymerase Chain Reaction in Clinical Samples of Lymphoproliferative Disorders. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_13

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  • DOI: https://doi.org/10.1007/978-3-642-84766-0_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84768-4

  • Online ISBN: 978-3-642-84766-0

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