Semiquantitative Analysis of Epstein-Barr Virus DNA by Polymerase Chain Reaction in Clinical Samples of Lymphoproliferative Disorders
Epstein-Barr virus (EBV) DNA was detected and semiquantitatively analyzed in clinical samples from patients with Hodgkin’s disease (HD), angioim-munoblastic lymphadenopathy (AILD), hairy cell leukemia (HCL), T-lymphoblastic lymphoma (TLL), familial gastric lymphoma, lymphocytic/ mixed thymoma, and reactive lymph node hyperplasia (HR). High numbers of viral DNA copies were detected in HD and AILD, whereas no or very few viral DNA copies were found in HCL and thymoma. One case of TLL and two cases of HR contained relatively high numbers of EBV DNA copies. In the case of TLL, the viral genomes probably originated from accessory B cells. The high amount of EBV DNA identified in the HD and AILD cases is not associated with the Bam W/Bam Z rearrangement known to disrupt viral latency. Technical details of amplification, oligonucleotide hybridization, and semiquantitative EBV DNA analysis are described. A sensitive detection system of the Bam W/Bam Z rearrangement is also presented.
KeywordsAlbumin Lymphoma EDTA Leukemia Agarose
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