Non-Mammalian Host Systems for the Expression of Mammalian Apolipoproteins
Recombinant mammalian apolipoproteins have been expressed in a variety of cultured mammalian cell lines (Reardon et al. 1986; Mallory et al. 1987; Blackhart et al. 1990; Yao et al. 1991), transgenic animals (Simonet et al. 1991), and bacteria (Vogel et al. 1985; Lalazar et al. 1988). These approaches have yielded considerable insight into the structure and function of several apolipoproteins but are rather limited for research or pharmaceutical purposes due to laborious technology or low yield often associated with these systems. This is particularly problematic if large scale production of biologically active lipoproteins for therapeutic purposes is to be attempted.
KeywordsCholesterol Toxicity Recombination Bromide Saccharomyces
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